Effects of enzymolysis on the functional ingredient contents and antioxidant activities of aqueous enzymatic extracts from Rice Bran and correlation analysis

Wang, Yuxin; Yu, Guoping; Sun, Aimin; Cai, Xing-Hang; Yue, Chonghui

doi:10.1007/s10068-015-0208-7

Cited by 5 publications

(2 citation statements)

References 30 publications

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“…The ABTS + • scavenging activity was assayed following the method of Wang et al [ 15 ] with minor modifications. ABTS + • was produced by mixing 7 mmol/L ABTS with 2.45 mmol/L potassium persulfate solution and reacting at room temperature under dark conditions for 12–16 h. Before use, solution obtained above was diluted with deionized water, and its absorbance at 734 nm was 0.70 ± 0.02.…”

Section: Methodsmentioning

confidence: 99%

Purification, Identification and Molecular Docking of Novel Antioxidant Peptides from Walnut (Juglans regia L.) Protein Hydrolysates

Fan

Mao

2022

Molecules

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Walnut protein isolate (WPI) was hydrolyzed using Alcalase for 0, 30, 60, 90, 120 and 150 min to investigate the effect of different hydrolysis times on the structure and antioxidant properties of walnut proteins. The identified peptides HADMVFY, NHCQYYL, NLFHKRP and PSYQPTP were used to investigate the structure-activity relationship by using LC-MS/MS and molecular docking. The kinetic equations DH = 3.72ln [1 + (6.68 E0/S0 + 0.08) t] were developed and validated to explore the mechanism of WIP hydrolysis by Alcalase. Structural characteristics showed that the UV fluorescence intensity and endogenous fluorescence intensity of the hydrolysates were significantly higher than those of the control. FTIR results suggested that the secondary structure gradually shifted from an ordered to a disordered structure. Enzymatic hydrolysis containing much smaller molecule peptides than WPI was observed by molecular weight distribution. In vitro, an antioxidant test indicated that Alcalase protease hydrolysis at 120 min showed more potent antioxidant activity than hydrolysates at other hydrolysis times. In addition, four new antioxidant peptides were identified by LC-MS/MS. Molecular docking indicated that these peptides could interact with ABTS through interactions such as hydrogen bonding and hydrophobic interactions. Thus, WPI hydrolysates could be used as potential antioxidants in the food and pharmaceutical industries.

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Section: Methodsmentioning

confidence: 99%

Purification, Identification and Molecular Docking of Novel Antioxidant Peptides from Walnut (Juglans regia L.) Protein Hydrolysates

Fan

Mao

2022

Molecules

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“…The DPPH and ABTS scavenging activity assays were determined according to the modified methods of Wang et al [ 14 ]. Here, 2.5 mL of a 38 μL DPPH methanolic solution and 0.5 mL of the respective extracts (the extraction was obtained by homogenizing 10 g millet bran and 100 mL ethanol) were mixed to determine the DPPH scavenging activity.…”

Section: Methodsmentioning

confidence: 99%

Effect of Mechanical Grinding on the Physicochemical, Structural, and Functional Properties of Foxtail Millet (Setaria italica (L.) P. Beauv) Bran Powder

Liu

Zhu

Zhang

2022

Foods

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This study investigated the functional, physicochemical, and structural characteristics of foxtail millet bran powder with different particle sizes. The morphological analysis revealed that the surface roughness declined in conjunction with the particle sizes of the millet bran powder. The Fourier-transform infrared (FTIR) spectra showed that none of the samples generated any additional chemical functional groups. A decrease in the particle sizes of the millet bran powder increased their dissemination and surface areas, as well as the bulk density, tap density, water-holding capacity (WHC), angle of repose (θ) and angle of slide (α), and peak temperature, while the oil holding capacity (OHC) and crystallinity index (CI) value declined. Moreover, fine millet bran powder (54.7 μm) exhibited a higher protein, fat, soluble dietary fiber (SDF), total phenolic content, and antioxidant capacity than its coarse counterpart.

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Enzymolysis-based ultrasound extraction and antioxidant activities of polyprenol lipids from Ginkgo biloba leaves

Zhang

Wang

Tao

et al. 2016

Process Biochemistry

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Effects of enzymolysis on the functional ingredient contents and antioxidant activities of aqueous enzymatic extracts from Rice Bran and correlation analysis

Cited by 5 publications

References 30 publications

Purification, Identification and Molecular Docking of Novel Antioxidant Peptides from Walnut (Juglans regia L.) Protein Hydrolysates

Purification, Identification and Molecular Docking of Novel Antioxidant Peptides from Walnut (Juglans regia L.) Protein Hydrolysates

Effect of Mechanical Grinding on the Physicochemical, Structural, and Functional Properties of Foxtail Millet (Setaria italica (L.) P. Beauv) Bran Powder

Enzymolysis-based ultrasound extraction and antioxidant activities of polyprenol lipids from Ginkgo biloba leaves

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