Effects of epigallocatechin-3-gallate on the healing of extraction sockets with a periapical lesion: A pilot study in dogs

Abstract

The aim of this study was to characterize the healing process of extraction sockets with a periapical lesion following immediate graft with collagenated bovine bone mineral (CBBM) soaked with epigallocatechin-3-gallate (EGCG). Following induction of periapical lesions in premolars in five dogs, treatment of extraction sockets was divided into three groups: control (no treatment) and two test groups, CBBM with or without EGCG. 3D reconstruction and superimposition of the digital images were used to measure the dimensional changes in alveolar ridge. Histologic specimen was evaluated in all groups. The horizontal ridge widths at 4-mm level were wider in both test groups (3.3 ± 1.7 mm in CBBM; 3.0 ± 1.7 mm in CBBM+EGCG) than in the control group (1.7 ± 2.4 mm). Fibrosis and limited new bone formation were observed in the apical regions of test groups; however, the extent of fibrosis was less in the CBBM+EGCG group. Within the limitations of this study, it was conjectured that adjunctive use of EGCG with CBBM can be a candidate biomaterial in grafting of extraction socket with periapical lesion. Bone regeneration at the coronal region of the CBBM grafted socket might not be influenced by the presence of a periapical lesion.

Green Tea Extracts Epigallocatechin-3-gallate for Different Treatments

“…Hong et al studied the healing process of extraction socket with the transplantation of collagenated bovine bone mineral (CBBM) soaked with EGCG, under the condition of injury of the dental periapical of dogs. A research indicated that after the treatment of EGCG, reduced inflammatory response and reduced alveolar bone absorption were observed [ 79 ]. Similarly as the dog as the experimental object, Shin et al found that the experimental group with EGCG is more beneficial to dehiscence defect healing with increased bone remodeling [ 80 ].…”

Polyphenols in Dental Applications

“…Epigallocatechin-3-gallate (EGCG) is a polyphenol which has antibacterial and antioxidant activities [40,41] as will be described in Section 2.3. In addition to those properties, EGCG was incorporated in dental adhesive-resins for its inhibitory effects of matrix-metalloproteinases (MMPs) and cysteine cathepsins.…”

Application of green tea extracts epigallocatechin‐3‐gallate in dental materials: Recent progress and perspectives

“…Collagen bovine bone mineral (CBBM) is a natural polymer material that has been widely used in bone regeneration. Ji‐Youn Hong et al found that the immediate transplantation of CBBM soaked in EGCG into the extraction sockets with a periapical lesion could reduce the extent of fibrosis and better promote bone regeneration than pure CBBM, which means that the combination of EGCG and CBBM can be used as a candidate biomaterial for the healing of extraction sockets (Hong et al, 2015).…”