Effects of Estriol on the Proliferation and Differentiation of Human Osteoblastic MG‐63 Cells

Abstract

Estriol has been showed to prevent bone loss in osteoporotic rats and postmenopausal women, but the mechanisms remain unclear. In the present study, we evaluated the effect of estriol on osteoblastic MG-63 cells in vitro, and compared its action with 17beta-estradiol (E2). Cell proliferation was determined by measuring total cell numbers and [3H]thymidine incorporation. Cell function was studied by measuring alkaline phosphatase (ALP) activity and secreted osteocalcin. Our data showed that estriol stimulated MG-63 cells proliferation in a dose-dependent manner, but had no influence on ALP activity in MG-63 cells and osteocalcin production. Compared with estriol treatment, E2 showed a stronger proliferation. Estrogen receptor (ER) alpha and beta expression in MG-63 cells can be detected by Western immunoblot analysis, and the proliferative response to E2 and estriol can be all abrogated by ER antagonist ICI 182,780. In conclusion, estriol stimulates osteoblastic MG-63 cells proliferation, but has no effects on differentiation. The proliferative response to estriol is mediated by the ER. These results suggest that estriol has an effect on osteoblastic proliferation, and this may contribute to its actions on prevention of bone loss.

Comparative effect of seeds of Rhynchosia volubilis and soybean on MG-63 human osteoblastic cell proliferation and estrogenicity

“…1). In the control cells (C), ERa and ERh proteins were both expressed, which is consistent with previous reports (Vidal et al, 1999;Luo and Liao, 2003). When the cells were treated with E 2 or isoflavone standards, ERa expression was markedly increased.…”

Oligodendrocyte Progenitor Proliferation and Maturation Is Differentially Regulated by Male and Female Sex Steroid Hormones

“…The effect of estrogens on proliferation has been well studied in other cellular systems, such as breast cancer cells [Mousavi and Adlercreutz, 1992;Chalbos et al, 1982;Hurd et al, 1997] and osteoblasts [Luo and Liao, 2003]. However, other reports have shown that estrogen treatment of breast cancer cells results in growth arrest [Seeger et al, 2003].…”

Protective effect of porcine placenta in a menopausal ovariectomized mouse

“…ALP activity is dependent upon both ERK pathway and ER activation in MG-63 cells (Prouillet et al 2004). Estriol stimulated MG-63 cells proliferation via ER and prevented bone loss (Luo & Liao 2003). Equol, a phytoestrogen, promoted the bone formation increasing ALP activity and the MG-63 cells proliferation through ER activation (Wang et al 2014).…”