Effects of Ethinylestradiol and the Fungicide Prochloraz on Metamorphosis and Thyroid Gland Morphology in Rana temporaria~!2009-09-28~!2010-02-25~!2010-05-17~!

Brande‐Lavridsen, Nanna; Christensen‐Dalsgaard, Jakob; Korsgaard, Bodil

doi:10.2174/1874336601003020007

Cited by 25 publications

(17 citation statements)

References 95 publications

(156 reference statements)

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“…and Hyperolius spp.) demonstrated by development acceleration or retardation (Brande‐Lavridsen, Christensen‐Dalsgaard, & Korsgaard, ; Degitz et al, ; Opitz et al, , among others). Some papers have also investigated the biological consequences of endocrine disruption in non‐model species to understand the origin of evolutionary novelties (Bloom et al, ; Jennings & Hanken, ; Laslo, Denver, & Hanken, ; Rose & Cahill, ).…”

Section: Introductionmentioning

confidence: 99%

Differences in responsiveness and sensitivity to exogenous disruptors of the thyroid gland in three anuran species

2019

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Anuran larval development comprises tissues/organs/systems that are: exclusively of larvae, able to be remodelled, and those of postmetamorphic stages. Also, the anuran larval development is characterized by inter‐related parameters: time, size and shape forming part of growth and differentiation. The anuran metamorphosis starts when growth and differentiation achieve a threshold that differs among species since it is regulated by a number of external (environmental) and internal (hormonal) processes. Here we explore the consequences of exogenous disruptors on the thyroid gland (e.g., methimazole and thyroxine as T4) of three species by immersing premetamorphic tadpoles in predetermined concentrations of the disruptors for short periods (10 or 16 days). The species were Pleurodema borellii, Leptodactylus chaquensis, and Dermatonotus muelleri, which all breed in small temporary ponds during the summer, but differ in their ecomorphology. The experiments were conducted to evaluate the effects of these substances on larval development (based in Gosner larval stages), morphometric variation in body parameters (snout‐vent and total length by larval stages), and thyroid gland histopathology at the end of the assays. In P. borelli and L. chaquensis, methimazole produces significant increment of size measurements (nonparametric Kruskal–Wallis, p < .05) during stages of digit differentiation and induced thyroid gland hypertrophy. In the three species, T4 exposure accelerated limb development and caused atrophy of thyroid gland. Prolonged T4 exposure in L. chaquensis and D. muelleri triggered metamorphic transformation in the gut and skull cartilages. Discussion about interspecific differences in responsiveness and sensitivity elucidates the importance of hormonal signals to morphological evolution.

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Section: Introductionmentioning

confidence: 99%

Differences in responsiveness and sensitivity to exogenous disruptors of the thyroid gland in three anuran species

2019

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“…In P. nattereri, tadpoles exposed to 0.01 mg.L À1 at 36 C presented more advanced stages than those from the control group at 28 C after eight days. It is already known that changes of temperature (Morand et al, 1997) and the presence of chemicals (Brande-lavridsen et al, 2010;Peltzer et al, 2013b) in the environment can alter the Fig. 4.…”

Section: Discussionmentioning

confidence: 99%

Combined effects of temperature and clomazone (Gamit®) on oxidative stress responses and B-esterase activity of Physalaemus nattereri (Leiuperidae) and Rhinella schneideri (Bufonidae) tadpoles

et al. 2017

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Temperature is an important factor influencing the toxicity of chemicals in aquatic environments. Neotropical tadpoles experience large temperature fluctuations in their habitats and many species are distributed in areas impacted by agriculture. This study evaluated the effects caused by the exposure to clomazone (Gamit) at different temperatures (28, 32 and 36 °C) on biochemical stress responses and esterase activities in Physalaemus nattereri and Rhinella schneideri tadpoles. Results evidenced that temperature modulates the effects of clomazone on biochemical response of tadpoles. Antioxidant enzymes, including catalase, superoxide dismutase (SOD), and glucose-6-phosphate dehydrogenase had their activities increased by clomazone in P. nattereri treated at higher temperatures. The biotransformation enzyme glutathione-S-transferase (GST) was also induced by clomazone at 32 and 36 °C. In R. schneideri, clomazone failed to alter antioxidant enzymes at 28 °C, but SOD and GST were increased by clomazone at higher temperatures after three days. All enzymes had their activities returned to the control levels after eight days in R. schneideri. Lipid peroxidation was induced in both species exposed to clomazone at 32 and 36 °C, but not at 28 °C. Acetylcholinesterase was not sensitive to clomazone and temperature, while most treatments impaired carboxylesterase activity. Integrated biomarker response (IBR) was notably induced by temperature in both species, and a synergic effect of temperature and clomazone was mostly observed after three days of exposure. These findings imply that tadpoles from tropical areas may present differential responses in their physiological mechanism linked to antioxidant defense to deal with temperature fluctuations and agrochemicals presence in their habitats.

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“…No alterations in metamorphosis were observed in tadpoles of R. schneideri for either period. Amphibian larvae have the ability to accelerate metamorphosis in response to deteriorating conditions in the environment, such as the presence of chemicals, as well as alterations of temperature (Brande-lavridsen et al, 2010;Morand et al, 1997;Murata and Yamauchi, 2005;Peltzer et al, 2013). Exposure to elevated temperatures usually reduces the time needed to complete metamorphosis for many amphibian species (Morand et al, 1997).…”

Section: Discussionmentioning

confidence: 99%

Influence of temperature on the antioxidant responses and lipid peroxidation of two species of tadpoles (Rhinella schneideri and Physalaemus nattereri) exposed to the herbicide sulfentrazone (Boral 500SC®)

Freitas

Teresa

Almeida

2017

Comparative Biochemistry and Physiology Part C: Toxicology &

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Amphibians can experience large temperature fluctuations in their habitats, especially during the larval stage, when tadpoles are restricted to small and ephemeral ponds. Changes in water temperature can alter development, metabolism and behaviour of cold-blooded animals but also the toxicokinetics of chemicals in the environment. In Brazil, pesticides application is intensified during the rainy season, which is the period of reproduction for many amphibian species. We evaluated here the influence of temperature (28, 32, and 36°C) on the toxicity of the herbicide sulfentrazone (Boral®SC) in tadpoles of Physalaemus nattereri and Rhinella schneideri, by analysis of oxidative stress biomarkers. Exposure of tadpoles to sulfentrazone altered the antioxidant enzymes activities and induced lipid peroxidation with temperature-associated responses. Catalase, superoxide dismutase and glucose-6-phosphate dehydrogenase (G6PDH) were impaired by combined effect of temperature and sulfentrazone in both species. G6PDH was increased in most groups exposed to 36°C. Biotransformation enzyme glutathione-S-transferase had more evident alterations in P. nattereri at higher temperatures and changes in tGSH contents presented different patterns between the species. Lipid peroxidation was particularly induced in tadpoles of P. nattereri. Integrated biomarker response (IBR) index indicated a synergic effect of temperature and sulfentrazone for tadpoles of P. nattereri, while the IBR was mainly influenced by temperature in R. schneideri. Our study showed that temperature modulates biochemical responses in tadpoles exposed to sulfentrazone with a species-specific pattern. These findings imply that the effects of abiotic factors should be taken into account to evaluate the real risks of exposure of amphibians to commonly used pesticides.

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Effects of Ethinylestradiol and the Fungicide Prochloraz on Metamorphosis and Thyroid Gland Morphology in Rana temporaria~!2009-09-28~!2010-02-25~!2010-05-17~!

Cited by 25 publications

References 95 publications

Differences in responsiveness and sensitivity to exogenous disruptors of the thyroid gland in three anuran species

Differences in responsiveness and sensitivity to exogenous disruptors of the thyroid gland in three anuran species

Combined effects of temperature and clomazone (Gamit®) on oxidative stress responses and B-esterase activity of Physalaemus nattereri (Leiuperidae) and Rhinella schneideri (Bufonidae) tadpoles

Influence of temperature on the antioxidant responses and lipid peroxidation of two species of tadpoles (Rhinella schneideri and Physalaemus nattereri) exposed to the herbicide sulfentrazone (Boral 500SC®)

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