Effects of Ethrel on Organogenesis of Protocorm-like bodies in Dendrobium kingianum In vitro

Abstract

This study attempted to understand the effects of BAP and ethrel on organogenesis of protocorm-like bodies (PLBs) in Dendrobium kingianum under white fluorescent lamps in vitro. BAP is the most effective cytokinin for multiplication in plant tissue culture. Ethrel regulates many aspects of plant morphogenesis. The highest number of PLBs (13.1) as against in control (8.1) and the highest number of developing shoots (2.1) was recorded in the medium containing 0.1 mg/l BAP combined with 1 mg/l ethrel. Increase in fresh weight showed higher values in same combination. Low concentration of BAP alone increased in the number of PLBs but showed inhibitory effects on shoot formation. On the other hand low concentration of ethrel alone increased both number of PLBs and shoots after 4 weeks in vitro.Dendrobium is popular in the international floriculture industry due to its floriferous flower sprays, wide spectrum of colors, sizes and shapes; year-round availability and long flowering life ( Kuehnle 2007, Khosravi et al. 2009). BAP is the most effective cytokinin for multiplication followed by Kn and 2-ip (Hu and Wang 1983, Schuch andErig 2005). Ethylene is unique among plant hormones; it is a simple hydrocarbon that affects growth, differentiation, and senescence in plants in concentrations as low as 0.01 µl/l (Reid 1995). Ethylene regulates many aspects of plant morphogenesis. Growth and development of cells cultured in vitro are largely dependent on the presence of phytohormones. Hence, modification of phytohormone composition and interaction in the nutrient medium has been the primary strategy to manipulate morphogenesis in vitro. Research on specific effects of ethylene on cell division is rather limited. From the

Adventitious organogenesis and phytochemical composition of Madonna lily (Lilium candidum L.) in vitro modeled by different light quality

“…A long-lasting application of blue light enhances the synthesis of ALA and eliminates the unfavorable effect of red light in this respect (Fan et al 2013). The literature data confirm the inhibitory effect of red light on the production of chlorophyll that we also observed in our study (Li et al 2010;Fan et al 2013;Habiba et al 2014;Bello-Bello et al 2016;Coelho et al 2021), and carotenoids (Liu et al 2011;Fan et al 2013;Pawłowska et al 2018b;Coelho et al 2021) in in vitro plant cultures. RBUV light also inhibited the synthesis of photosynthetic pigments, both chlorophylls and carotenoids, in L. candidum cultures.…”

LEDs Combined with CHO Sources and CCC Priming PLB Regeneration of Phalaenopsis

“…Conversely, BW-trehalose produced the second largest number of shoots (Table 2). LEDs have been successfully applied in vitro in various plant species [20,[29][30][31][32][33]. The ideal light stipulation for each plant species is unique.…”

“…A number of in vitro studies have reported vigorous plant growth under LEDs. LED lights have been previously used for PLB organogenesis in Cymbidium finlaysonianum [29], Dendrobiumkingianum [30,31], hybrid Cymbidium [32,33], and plantlet regeneration in gerbera [34]. A series of studies have already been conducted to improve the tissue culture of Phalaenopsis using a number of factors.…”

Light and Plant Growth Regulators on In Vitro Proliferation

“…Photosynthetic capacity was highest in Betula pendula Roth [ 154 ] and in chrysanthemum ( Dendranthema grandiflorum ) [ 42 ] when the plantlets were exposed to BL as compared to RL. In Dendrobium kingianum , the average number of PLBs and the chlorophyll content were highest under B-LEDs, in contrast to the explants cultured under R-LEDs where the highest shoot formation and fresh weight were observed [ 99 ]. Likewise, a study of Oncidium PLBs by Mengxi et al [ 90 ] showed that chlorophyll a and b and carotenoid levels and the greatest growth were detected under B-LEDs.…”