Effects of Ethyl Pyruvate and Other α-Keto Carboxylic Acid Derivatives in a Rat Model of Multivisceral Ischemia and Reperfusion

Cruz, Ruy J.; Harada, Tomoyuki; Sasatomi, Eizaburo; Fink, Mitchell P.

doi:10.1016/j.jss.2009.07.008

Cited by 28 publications

(27 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…16) Our data presented in Fig. 3 are consistent with the physicochemical property hypothesis related to differing lipophilicity.…”

Section: Discussionsupporting

confidence: 88%

“…16) Rats subjected to multivisceral ischemia as a reperfusion model have shown that benzoylformic acid (the anion of a carboxylic acid) decreased damage similarly to EtP. Our data confirm these observations.…”

Section: Discussionsupporting

confidence: 86%

“…The well-described differences in the pharmacological effects of EtP and NaP [10][11][12][13][14][15][16] may reflect the greater lipophilicity of EtP. 16) Our data presented in Fig.…”

Section: Discussionmentioning

confidence: 70%

“…Despite its greater stability, EtP also appeared to be more effective than sodium pyruvate (NaP) under various stress conditions. [10][11][12][13][14][15][16] An in vivo model of LPS-induced hepatocellular injury indicated both EtP and NaP to have protective properties, although EtP was more efficacious.…”

mentioning

confidence: 99%

See 3 more Smart Citations

Higher Hypochlorous Acid Scavenging Activity of Ethyl Pyruvate Compared to Its Sodium Salt

Olek

Ziółkowski

Kaczor

et al. 2011

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

“…16) Our data presented in Fig. 3 are consistent with the physicochemical property hypothesis related to differing lipophilicity.…”

Section: Discussionsupporting

confidence: 88%

Section: Discussionsupporting

confidence: 86%

“…The well-described differences in the pharmacological effects of EtP and NaP [10][11][12][13][14][15][16] may reflect the greater lipophilicity of EtP. 16) Our data presented in Fig.…”

Section: Discussionmentioning

confidence: 70%

mentioning

confidence: 99%

See 2 more Smart Citations

Higher Hypochlorous Acid Scavenging Activity of Ethyl Pyruvate Compared to Its Sodium Salt

Olek

Ziółkowski

Kaczor

et al. 2011

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

“…The in situ method was accompanied by a determination of permeability in isolated ileal segments following a method described elsewhere (Cruz et al, 2011). Briefly, the harvested small intestine was carefully cleaned of luminal debris with ice-cold saline and inverted over a plastic pipette.…”

Section: Methodsmentioning

confidence: 99%

Remediation of Hemorrhagic Shock-Induced Intestinal Barrier Dysfunction by Treatment with Diphenyldihaloketones EF24 and CLEFMA

Yadav

Hussain

Sahoo

et al. 2014

The Journal of Pharmacology and Experimental Therapeutics

View full text Add to dashboard Cite

Gut is very sensitive to hypoperfusion and hypoxia, and deranged gastrointestinal barrier is implicated in systemic failure of various organs. We recently demonstrated that diphenyldihaloketone EF24 [3,5-bis(2-fluorobenzylidene)piperidin-4-one] improves survival in a rat model of hemorrhagic shock. In this study, we tested EF24 and its other analog CLEFMA (4-[3,5-bis(2-chlorobenzylidene)-4-oxo-piperidine-1-yl]-4-oxo-2-butenoic acid) for their effect on intestinal barrier dysfunction in hypovolemic shock. Hypovolemia was induced in rats by withdrawing 50% of blood. EF24 or CLEFMA (0.4 mg/kg i.p.) treatment was provided, without volume resuscitation, after 1 hour of hemorrhage. Ileum was collected 5 hours after the treatment to investigate the expression of tight junction proteins (zonula occludens, claudin, and occludin) and epithelial injury markers [myeloperoxidase, ileal lipid-binding protein (ILBP), CD163, and plasma citrulline]. The ileal permeability for dextran-fluoroisothiocyanate and Evan's blue dye was determined. EF24 and CLEFMA reduced the hypovolemiainduced plasma citrulline levels and the ileal expression of myeloperoxidase, ILBP, and CD163. The drugs also restored the basal expression levels of zonula occludens, claudin, and occludin, which were substantially deranged by hypovolemia. In ischemic ileum, the expression of phospho(tyrosine)-zonula occludens-1 was reduced, which was reinstated by EF24 and CLEFMA. In contrast, the drug treatments maintained the hypovolemia-induced expression of phospho(threonine)-occludin, but reduced that of phospho(tyrosine)-occludin. Both EF24 and CLEFMA treatments reduced the intestinal permeability enhanced by hypovolemia. EF24 and CLEFMA attenuate hypovolemic gut pathology and protect barrier function by restoring the status of tight junction proteins. These effects were observed in unresuscitated shock, implying the benefit of EF24 and CLEFMA in prehospital care of shock.

show abstract

Prophylactic effect of ethyl pyruvate on renal ischemia/reperfusion injury mediated through oxidative stress

et al. 2018

View full text Add to dashboard Cite

Effects of Ethyl Pyruvate and Other α-Keto Carboxylic Acid Derivatives in a Rat Model of Multivisceral Ischemia and Reperfusion

Cited by 28 publications

References 31 publications

Higher Hypochlorous Acid Scavenging Activity of Ethyl Pyruvate Compared to Its Sodium Salt

Higher Hypochlorous Acid Scavenging Activity of Ethyl Pyruvate Compared to Its Sodium Salt

Remediation of Hemorrhagic Shock-Induced Intestinal Barrier Dysfunction by Treatment with Diphenyldihaloketones EF24 and CLEFMA

Prophylactic effect of ethyl pyruvate on renal ischemia/reperfusion injury mediated through oxidative stress

Contact Info

Product

Resources

About