Effects of Excess Pantothenic Acid Administration on the Other Water-Soluble Vitamin Metabolisms in Rats

Abstract

SummaryTo acquire the data concerning the tolerable upper intake level which prevents health problems from an excessive intake of pantothenic acid, an animal experiment was done. Rats of the Wistar strain (male, 3wk old) were fed on a diet which contains 0%, 0.0016% (control group), 1%, or 3% calcium pantothenate for 29d. The amount of weight increase, the food intake, and the organ weights were measured, as well as the pantothenic acid contents in urine, the liver and blood. Moreover, to learn the influence of excessive pan tothenic acid on other water-soluble vitamin metabolism, thiamin, riboflavin, a vitamin B6 catabolite, the niacin catabolites, and ascorbic acid in urine were measured. As for the 3% addition group, enlargement of the testis, diarrhea, and hair damage were observed, and the amount of weight increase and the food intake were less than those of the control group. However, abnormality was not seen in the 1% addition group. The amount of pantothenic acid in urine, the liver, and blood showed a high correlation with intake level of pantothenic acid. It was only for 4-pyridoxic acid, a vitamin B6 catabolite, in urine that a remarkable dif ference was observed against the control group. Moreover, the (2-Py+4-Py)/MNA excretion ratio for these metabolites of the nicotinamide also indicated a low value in the 3% pan tothenic acid group. As for the calcium pantothenate, it was found that the 3% level in the diet was the lowest-observed-adverse-effect-level (LOAEL) and the 1% level was the no observed-adverse-effect-level (NOAEL).

Effects of Pantothenic Acid on Testicular Function in Male Rats

“…The reason why the testicular weight is different by body weight is unclear. Supporting the present findings, a previous paper reported similar results for the changes in testicular weight in the PaA-deficient rat (Shibata K. et al) [30].…”

“…Corn oil was purchased from Ajinomoto (Tokyo, Japan). Gelatinized cornstarch, the mineral mixture (AIN-93M) [30] and the vitamin mixture (AIN-93VX containing 25% choline bitartrate) [30] were obtained from Oriental Yeast Co., Ltd. (Tokyo, Japan).…”

Effects of Excess Biotin Administration on the Growth and Urinary Excretion of Water-Soluble Vitamins in Young Rats

“…27,28) In the present study, an extremely high dose of biotin representing more than a 0.80% in the diet caused death, and more than 0.08% biotin-added diet retarded the growth of young rats. These results suggest that an excess biotin intake might cause some adverse effects on humans, and that setting UL for biotin would be important to prevent such dietary biotin-induced adverse effects.…”

The Interactive Effect of Dietary Water-Soluble Vitamin Levels on the Depression of Gonadal Development in Growing Male Rats Kept under Disturbed Daily Rhythm

“…It has also been reported that a mild PA defi ciency diet (1/100 of normal level) decreases the serum triglyceride level and free fatty acid level after a 12-h fast (18). On the other hand, in an experiment with excess PA, a 3% PA diet (approximately 3,000 times the normal PA content) induced reductions of food intake and body weight, diarrhea and enlarged lungs, but a 1% PA diet (approximately 600 times the normal content) did not induce these phenomena (19). In the present study, the dietary PA level was six times the normal content, a high but not excessive level, and a high-PA diet increased gonadal weight of rats in the D-groups but decreased gonadal weight of rats in the N-groups.…”