The Interactive Effect of Dietary Water-Soluble Vitamin Levels on the Depression of Gonadal Development in Growing Male Rats Kept under Disturbed Daily Rhythm

Hanai, Miho; ESASHI, Takatoshi

doi:10.3177/jnsv.58.230

Cited by 6 publications

(5 citation statements)

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“…This protein participates in the vitamin B 12 transport and absorption in testes, suggesting that the steroidogenic activity seems to depend on the transport of vitamin B 12 in Leydig cells (66). Except for a study in which rats fed with diet containing high levels of vitamin B 12 showed high serum testosterone levels (68), studies showing preventive effect of vitamin B 12 against androgenic disruption are inexistent in the literature. Our results showed that vitamin B 12 is able to maintain the steroidogenic activity as well as the serum testosterone at normal levels in rats following treatment with an androgen disrupter (Figure 8).…”

Section: Discussionmentioning

confidence: 99%

Vitamin B12 Prevents Cimetidine-Induced Androgenic Failure and Damage to Sperm Quality in Rats

et al. 2019

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Cimetidine, used as an anti-ulcer and adjuvant treatment in cancer therapy, causes disorders in the male reproductive tract, including steroidogenesis. However, its effect on sperm quality and male fertility has been poorly addressed. Since vitamin B 12 has demonstrated to recover spermatogonia number and sperm concentration in cimetidine-treated rats, we evaluated the impact of cimetidine on sperm quality and fertility potential and whether vitamin B 12 is able to prevent the harmful effect of this drug on steroidogenesis and sperm parameters. Adult male rats were treated for 52 consecutive days as follows: cimetidine group (100 mg/kg of cimetidine), cimetidine/vitamin B 12 group (100 mg/kg of cimetidine + 3 μg vitamin B 12 ), vitamin B 12 group (3 μg vitamin B 12 ) and control group (saline). Serum testosterone levels and immunofluorescence associated to western blot for detection of 17β-HSD6 were performed. Sperm morphology and motility, mitochondrial activity, acrosome integrity, DNA integrity by Comet assay, lipid peroxidation as well as fertility potential were analyzed in all groups. Apoptotic spermatids were also evaluated by caspase-3 immunohistochemistry. In the cimetidine-treated animals, reduced serum testosterone levels, weak 17β-HSD6 levels and impaired spermiogenesis were observed. Low sperm motility and mitochondrial activity were associated with high percentage of sperm tail abnormalities, and the percentage of spermatozoa with damaged acrosome and DNA fragmentation increased. MDA levels were normal in all groups, indicating that the cimetidine-induced changes are associated to androgenic failure. In conclusion, despite the fertility potential of rats was unaffected by the treatment, the sperm quality was significantly impaired. Therefore, considering a possible sperm-mediated transgenerational inheritance, the long term offspring health needs to be investigated. The administration of vitamin B 12 to male rats prevents the androgenic failure and counteracts the damage inflicted by cimetidine upon sperm quality, indicating that this vitamin may be used as a therapeutic agent to maintain the androgenic status and the sperm quality in patients exposed to androgen disrupters.

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Section: Discussionmentioning

confidence: 99%

Vitamin B12 Prevents Cimetidine-Induced Androgenic Failure and Damage to Sperm Quality in Rats

et al. 2019

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“…We have been investigating the effects of various nutrients on sexual organ development in rats kept under constant darkness (5)(6)(7)(8)(9)(10)(11)(12). The present study used a model in which the daily rhythm was free-running rather than a 24-h rhythm.…”

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confidence: 99%

Comparison of the Effects of Dietary Protein on the Sexual Organ Development of Male Mice and Rats Kept under Constant Darkness

Hanai

Esashi

2013

J Nutr Sci Vitaminol

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The photoperiod has a regulatory effect on the sexual development of mammals (1, 2). Whitsett et al. reported that the development of the sexual organs of male deer mice was accelerated by a long-day regimen and inhibited by a short-day or constant darkness regimen (3).Sunlight is one of the external factors that synchronize the circadian rhythm (an approx. 24-h rhythm) to the 24-h rhythm when the light-dark cycle is 24 h. If a mammal is kept under constant darkness, its biological functions will operate according its own biological clock; that is, a free-running rhythm will develop (4).We have been investigating the effects of various nutrients on sexual organ development in rats kept under constant darkness (5-12). The present study used a model in which the daily rhythm was free-running rather than a 24-h rhythm. We have a long-range plan to develop the model for use in further basic research and in experiments using other types of rhythm, to obtain data that will identify the most suitable nutritional requirements for human beings, who frequently do not live in accord with a 24-h cycle.Some of our results by a factor analysis method showed that the sexual organ development of rats kept under constant darkness was inhibited by a low-protein diet, and the inhibition of sexual organ development was diminished by a sulfur amino acid-additive diet (5, 7). All of these studies were performed on rats, not mice.Recently, research about the daily rhythm and circadian rhythm has revealed that the clock genes control the biological rhythm (13). Furthermore, it was reported that the clock genes participate in the onset of lifestylerelated disease (14). There are also reports that the composition of diet and the meal times influence clock gene expression in the mouse liver (15,16). These studies all involved experimenting with mice.Since we had conducted nutritional experiments using rats given the AIN-based diet (AIN-76, AIN-93G) (17), we wanted to determine whether the nutritional data obtained during the constant darkness experiments using rats could also be applied to mice.Based on our previous experimental results, the diet we tested in the present study was low in protein, with a 9% casein level to which sulfur amino acid, cystine, was added or not added. These diets were given to rats and mice kept under constant darkness. The amount cystine was adjusted to the 9% casein level to avoid amino acid imbalance. MethodsAnimals. Twenty-four CD-1 (ICR) male mice and 24 Fischer strain (F344) male rats (purchased from Charles River Japan, Inc., Yokohama, Japan, at 3 wk of age) were preliminarily maintained for 4 d on the AIN- Note Comparison of the Effects of Dietary Protein on the Sexual Organ Development of Male Mice and Rats Kept under Constant DarknessMiho Hanai* and Takatoshi Esashi † Department of Nutrition and Life Science, Kanagawa Institute of Technology, Atsugi, Kanagawa 243-0292, Japan (Received May 7, 2013) Summary The purpose of this study was to determine whether the effects of dietary protein on sexual ...

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“…revealed that pyridoxine-deficient male rats have lower levels of testosterone [ 29 ]. Another in vivo system study conducted by Hanai and Esashi in 2012 revealed that the reduction in gonadal development in male rats, kept in darkness, improved when receiving adequate amounts of thiamine (vitamin B 1 ) and pyridoxine, and high amounts of pantothenic acid (vitamin B 5 ) [ 30 ]. Therefore, together, given that gonadal hormones, mainly testosterone, are required to achieve spermatogenesis [ 31 , 32 ], then the above indication suggests a vital contribution of pyridoxine in conserving normal gonadal function, and thus normal quantity of sperm.…”

Section: Discussionmentioning

confidence: 99%

Men with oligozoospermia had lower level of seminal plasma pyridoxine compared to normozoospermic men

Banihani¹,

Aljabali²

2022

Heliyon

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The Interactive Effect of Dietary Water-Soluble Vitamin Levels on the Depression of Gonadal Development in Growing Male Rats Kept under Disturbed Daily Rhythm

Cited by 6 publications

References 36 publications

Vitamin B12 Prevents Cimetidine-Induced Androgenic Failure and Damage to Sperm Quality in Rats

Vitamin B12 Prevents Cimetidine-Induced Androgenic Failure and Damage to Sperm Quality in Rats

Comparison of the Effects of Dietary Protein on the Sexual Organ Development of Male Mice and Rats Kept under Constant Darkness

Men with oligozoospermia had lower level of seminal plasma pyridoxine compared to normozoospermic men

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