Effects of exogenous gamma-aminobutyric acid on α-amylase activity in the aleurone of barley seeds

Sheng, Yanqing; Xiao, Huiyuan; Guo, Chiao; Wu, Hanshuo; Wang, Xiaojing

doi:10.1016/j.plaphy.2018.02.030

Cited by 33 publications

(22 citation statements)

References 69 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generally, exogenous GABA promotes germination [20,21] and enhances GABA absorption in orthodox seeds [20]. Unexpectedly, our analysis of recalcitrant chestnut seeds uncovered a transient decrease in endogenous GABA concentrations at 48 h following the GABA and VGB treatments (Figure 2A), which was inconsistent with the data generated during our recent investigation of GABA-and VGB-treated poplar stem fragments [37].…”

Section: Discussioncontrasting

confidence: 78%

“…However, the decrease in GABA after 48 h may be ascribed to the requirement of much more Glu for protein synthesis or rapid degradation of GABA back to the TCA cycle during primary root growth [8,51]. However, the application of 10 mM GABA inhibited chestnut seed germination and early primary root growth (Figure 1), which was inconsistent with the results of earlier investigations on barley [20] and H. ammodendron [21] seeds. Moreover, blocking GABA degradation with 100 µM VGB also had an inhibitory effect (Figure 1).…”

Section: Discussioncontrasting

confidence: 69%

“…Recent genetic and physiological studies have implied that GABA is involved in responses to abiotic stresses [46,47], as well as developmental processes, including pollen tube growth [48], primary/adventitious root growth [37,49], and seed germination [20,21]. The recent identification of a GABA receptor, aluminum-activated malate transporter (ALMT) [50], indicates that GABA is a signaling molecule and not just a metabolite [51].…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of GABA and Vigabatrin on the Germination of Chinese Chestnut Recalcitrant Seeds and Its Implications for Seed Dormancy and Storage

Chen

et al. 2020

Plants

View full text Add to dashboard Cite

Recalcitrant chestnut seeds are rich in γ-aminobutyric acid (GABA), which negatively regulates adventitious root development by altering carbon/nitrogen metabolism. However, little is known regarding the role of this metabolite in chestnut seeds. In this study, we investigated the effects of GABA changes on the germination of chestnut seeds treated with exogenous GABA and vigabatrin (VGB, which inhibits GABA degradation). Both treatments significantly inhibited seed germination and primary root growth and resulted in the considerable accumulation of H2O2, but the endogenous GABA content decreased before germination at 48 h. Soluble sugar levels increased before germination, but subsequently decreased, whereas starch contents were relatively unchanged. Changes to organic acids were observed at 120 h after sowing, including a decrease and increase in citrate and malate levels, respectively. Similarly, soluble protein contents increased at 120 h, but the abundance of most free amino acids decreased at 48 h. Moreover, the total amino acid levels increased only in response to VGB at 0 h. Accordingly, GABA and VGB altered the balance of carbon and nitrogen metabolism, thereby inhibiting chestnut seed germination. These results suggested that changes to GABA levels in chestnut seeds might prevent seed germination. The study data may also help clarify the dormancy and storage of chestnut seeds, as well as other recalcitrant seeds.

show abstract

Section: Discussioncontrasting

confidence: 78%

Section: Discussioncontrasting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Effects of GABA and Vigabatrin on the Germination of Chinese Chestnut Recalcitrant Seeds and Its Implications for Seed Dormancy and Storage

Chen

et al. 2020

Plants

View full text Add to dashboard Cite

show abstract

“…A receptor for GABA in plants was identified only in 2015 in an aluminium‐activated malate transporter (ALMT) protein (Ramesh et al, ), although evidence for signalling roles for GABA in plants had been already described before (Barbosa, Singh, Cherry, & Locy, ; Baum et al, ; Lancien & Roberts, ). A multitude of biological processes seem to be linked to GABA signalling, such as regulation of 14–3–3 genes and control of developmental processes such as germination and pollen tube growth (Lancien & Roberts, ; Palanivelu, Brass, Edlund, & Preuss, ; Sheng, Xiao, Guo, Wu, & Wang, ). This body of evidence delineates GABA as an important molecule in connecting primary metabolism to signalling in plant cells (Bown & Shelp, ; Seifikalhor et al, ).…”

Section: Introductionmentioning

confidence: 99%

γ‐Aminobutyric acid and related amino acids in plant immune responses: Emerging mechanisms of action

Tarkowski

Signorelli

Höfte

2020

Plant Cell & Environment

101

View full text Add to dashboard Cite

The entanglement between primary metabolism regulation and stress responses is a puzzling and fascinating theme in plant sciences. Among the major metabolites found in plants, γ-aminobutyric acid (GABA) fulfils important roles in connecting C and N metabolic fluxes through the GABA shunt. Activation of GABA metabolism is known since long to occur in plant tissues following biotic stresses, where GABA appears to have substantially different modes of action towards different categories of pathogens and pests. While it can harm insects thanks to its inhibitory effect on the neuronal transmission, its capacity to modulate the hypersensitive response in attacked host cells was proven to be crucial for host defences in several pathosystems. In this review, we discuss how plants can employ GABA's versatility to effectively deal with all the major biotic stressors, and how GABA can shape plant immune responses against pathogens by modulating reactive oxygen species balance in invaded plant tissues. Finally, we discuss the connections between GABA and other stress-related amino acids such as BABA (β-aminobutyric acid), glutamate and proline. K E Y W O R D SBABA, biotic stress, GABA, glutamate, hypersensitive response, primary metabolism, proline, ROS

show abstract

“…GABA and Linoleic Acid. GABA increased α-amylase gene expression by treating barley aleurone with exogenous GABA, especially α-amylase activity began to rise after about 24 h and reached a peak at 48 h [78]. The GABA content (mg/100 g) in 629 accessions of barley grains is 8:00 ± 3:92 mg/100 g, the highest up to 30:67 8:00 ± 3:92 mg/100 g [75].…”

Section: Tocolsmentioning

confidence: 99%

Molecular Mechanism of Functional Ingredients in Barley to Combat Human Chronic Diseases

Zeng

et al. 2020

Oxidative Medicine and Cellular Longevity

View full text Add to dashboard Cite

Barley plays an important role in health and civilization of human migration from Africa to Asia, later to Eurasia. We demonstrated the systematic mechanism of functional ingredients in barley to combat chronic diseases, based on PubMed, CNKI, and ISI Web of Science databases from 2004 to 2020. Barley and its extracts are rich in 30 ingredients to combat more than 20 chronic diseases, which include the 14 similar and 9 different chronic diseases between grains and grass, due to the major molecular mechanism of six functional ingredients of barley grass (GABA, flavonoids, SOD, K-Ca, vitamins, and tryptophan) and grains (β-glucans, polyphenols, arabinoxylan, phytosterols, tocols, and resistant starch). The antioxidant activity of barley grass and grain has the same and different functional components. These results support findings that barley grain and its grass are the best functional food, promoting ancient Babylonian and Egyptian civilizations, and further show the depending functional ingredients for diet from Pliocene hominids in Africa and Neanderthals in Europe to modern humans in the world. This review paper not only reveals the formation and action mechanism of barley diet overcoming human chronic diseases, but also provides scientific basis for the development of health products and drugs for the prevention and treatment of human chronic diseases.

show abstract

Effects of exogenous gamma-aminobutyric acid on α-amylase activity in the aleurone of barley seeds

Cited by 33 publications

References 69 publications

Effects of GABA and Vigabatrin on the Germination of Chinese Chestnut Recalcitrant Seeds and Its Implications for Seed Dormancy and Storage

Effects of GABA and Vigabatrin on the Germination of Chinese Chestnut Recalcitrant Seeds and Its Implications for Seed Dormancy and Storage

γ‐Aminobutyric acid and related amino acids in plant immune responses: Emerging mechanisms of action

Molecular Mechanism of Functional Ingredients in Barley to Combat Human Chronic Diseases

Contact Info

Product

Resources

About