Effects of experimental osteoporosis on alveolar bone loss in rats.

Moriya, Yoshitomo; Ito, Koichi; Murai, Seidai

doi:10.2334/josnusd.40.171

Cited by 55 publications

(56 citation statements)

References 20 publications

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“…24 A possible relationship between estrogen deficiency and alveolar bone loss without the induction of experimental of periodontitis was not verified in this study. Similarly, some studies on animals have observed that osteoporosis itself may not be capable of causing alveolar bone loss; 9,10,25 in contrast, other studies have shown that estrogen deficiency may have a direct effect on alveolar bone loss in the furcation region. 9 In the present study, estrogen deficiency for 90 days resulted in greater ligature-induced bone loss in rats.…”

Section: Discussionmentioning

confidence: 99%

Influence of different durations of estrogen deficiency on alveolar bone loss in rats

et al. 2011

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The purpose of this study was to morphometrically evaluate the influence of different durations of ovariectomy-induced estrogen deficiency on alveolar bone loss associated with ligature-induced bone loss in rats. Sixty female Wistar rats were randomly assigned to ovariectomy (OVX test group) or sham operation (SHAM control group). The OVX and SHAM groups were each distributed into three subgroups of ten rats each according to the duration of estrogen deficiency (30, 60 and 90 postoperative days). In all groups, for the last 30 days of the experimental period, cotton ligatures were placed around the cervix of the right upper second molar; the contralateral tooth was left unligated to serve as a control. The maxillary bones were removed, and the alveolar bone loss was analyzed by measuring the distance between the cementoenamel junction and the alveolar bone crest at the buccal site of the right upper second molar. A comparison between the ligated and unligated groups verified the presence of ligature-induced alveolar bone loss (p < 0.05). No significant differences were observed among the unligated groups (p > 0.05). A significant increase in bone loss was observed when ligation occurred 90 days after ovariectomy compared with the sham group (p < 0.05). These results demonstrate that long-term estrogen deficiency affects ligatureinduced alveolar bone loss.

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Section: Discussionmentioning

confidence: 99%

Influence of different durations of estrogen deficiency on alveolar bone loss in rats

et al. 2011

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“…An alternative would be to preserve the inferior molar in the SHAM group and promote a masticatory effect decrease by extracting this antagonist tooth only in ovariectomized groups, which could lead to different results. The results for trabecular volume of animals that were fed a low calcium diet were unexpected once the literature reports great bone loss using this methodology (8,11). It was observed that the LCD group mean was lower than the OVZ group; with no statistical significance, however.…”

Section: Discussionmentioning

confidence: 72%

“…The difficulty in obtaining osteopenic alterations in gnathic bones of rodents is greatly discussed. Many studies did not find differences between maxillary and mandibular bones of control animals sham-operated and ovariectomized (8,11). This is possibly due to the mastication type of these animals.…”

Section: Discussionmentioning

confidence: 94%

Effect of low-calcium diet and grind diet on bone turnover of ovariectomized female rats

Costa¹,

Leite²,

Prado³

et al. 2011

Med Oral

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Objective: The variety of methodologies used to investigate ovariectomized female rats shows different results, which makes a clinical application of these results difficult. The purpose of this work was to evaluate the effect of masticatory effort reduction and of low-calcium diet on maxillary bone turnover of ovariectomized female rats. Study Design: Eighty-four female rats were divided into four groups of 21 animals each as follows: SHAMsham-operated; OVZ -ovariectomized and fed a standard commercial diet; LCD -fed a low calcium diet, and GCD -fed a grind commercial diet. The inferior first molars were extracted bilaterally 15 days after the ovariectomy, and the animals were euthanized 3, 5 and 8 weeks after ovaries removal. The maxillae were embedded in methylmetacrilate. The results were submitted to analysis of variance. Results: The daily mineral apposition rate lowered with time and was not different between SHAM and OVZ groups. The trabecular bone volume of SHAM and OVZ animals was similar and decreased with time. The GCD animals presented the lowest means and the LCD the highest in comparison to the OVZ group. Conclusion: It was concluded that ovariectomy and a low calcium diet did not cause significant maxillary bone loss in the first molar region, and even in the absence of the antagonist tooth, they did not cause maxillary bone turnover. The grind commercial diet is a good alternative for the study of maxillary bone loss in ovariectomized female rats.

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“…3 Radiographic studies with rats have used mammography films, 5 dental radiography films, [10][11][12] intraoral 4 or oclusal, 12,13 and low kilovoltage X-ray sources. [14][15][16] These studies do not seem to have reached satisfactory results -as they were not employed in more recent studies -and other methods to obtain images, such as X-ray absorptiometry, bone densitometry [17][18][19][20][21] and micro X-ray CT [22][23][24][25] have been investigated. However, these methods require special installations for their use with animals and have a high operational cost.…”

Section: Discussionmentioning

confidence: 99%

Optimal parameters for lateral oblique radiographs of rat mandibles

Mahl

Fontanella²

2008

Dentomaxillofacial Radiology

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Objective: To establish optimal exposure parameters for lateral oblique radiographs of rat mandibles using an intraoral X-ray machine. Methods: A positioning apparatus, previously tested for its reproducibility, was used to obtain radiographs of four heads of formaldehyde-preserved Wistar rats. Radiographs were exposed at 50 kV and 8 mA using four size 2 films (Insight, Ektaspeed Plus, Ultraspeed and D-Speed; Eastman Kodak Co., Rochester, NY), two focal distances (30 cm and 60 cm) and seven exposure times (0.6 s, 0.7 s, 0.8 s, 1.0 s, 1.5 s, 2.0 s and 2.5 s). The radiographs were evaluated by two examiners and scored for image quality: 1, very poor; 2, poor; 3, fair; 4, good; and 5, excellent. The evaluations were repeated at 30 days. Results: Intraobserver reproducibility was substantial (kappa 5 0.798 and 0.667). Also, the two examiners showed substantial agreement (kappa 5 0.726). There was a significant difference (a 5 1%) between the scores for the films under study. At a 30 cm distance, Espeed films had mean scores of 4 and 5 at 0.8 s and 1 s exposure times, and D-speed films, at 1.5 s, 2 s and 2.5 s exposure times. At a 60 cm distance, the best results were found for Espeed films at 2.0 s and 2.5 s exposure times. Conclusion: The films under study did not show any significant difference in the quality of rat mandible images as long as exposure times were adequate to their sensitivity and focal distance.

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Effects of experimental osteoporosis on alveolar bone loss in rats.

Cited by 55 publications

References 20 publications

Influence of different durations of estrogen deficiency on alveolar bone loss in rats

Influence of different durations of estrogen deficiency on alveolar bone loss in rats

Effect of low-calcium diet and grind diet on bone turnover of ovariectomized female rats

Optimal parameters for lateral oblique radiographs of rat mandibles

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