Effects of external factors on the amino acid pool of <i>Penicillium griseofulvum</i>

Bent, KJ; Morton, A. G.

doi:10.1042/bj0920270

Cited by 16 publications

(2 citation statements)

References 19 publications

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“…This suggests that only mycelium formed in the presence of excess tryptophan is conditioned, at a later stage of development, to a high level of alkaloid synthesis. Although the effect might be attributed to a difference in nutrient uptake between older hyphae and growing tips, studies with other fungi (5,6,36,37) have indicated little distinction between different parts of the mycelium in assimilation and metabolism of amino acids. Tryptophan appears to be taken up readily by growing or resting mycelium of Claviceps strain HLX 123.…”

Section: Discussionmentioning

confidence: 95%

Effect of tryptophan on alkaloid biosynthesis in cultures of a Claviceps species

Lc¹

1970

Can. J. Microbiol.

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VINING, L. C. 1970. Effect of tryptophan on alkaloid biosynthesis in cultures of a Claviceps species.Can. J. Microbial. 16: 473480. Addition of tryptophan to cultures of Claviceps strain HLX 123 caused a large increase in alkaloid production. For maximum effect a supplement of at least 250 mg/l was required within 1 day after inoculation. When ~-tryptophan-B-l4C was used the labeled amino acid accumulated in the mycelium until alkaloid synthesis began, near the end of the growth phase. Intracellular tryptophan was then rapidly incorporated into alkaloids which were excreted into the culture fluid. L-Tryptophan-e-14C added to cultures during the period of rapid alkaloid synthesis was efficiently incorporated into alkaloids, but caused only a small increase in yield. The results suggest that tryptophan stimulates alkaloid production mainly through an increase in activity of the alkaloid-synthesizing enzyme system. The role of tryptophan in the regulatory process is discussed.

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Section: Discussionmentioning

confidence: 95%

Effect of tryptophan on alkaloid biosynthesis in cultures of a Claviceps species

Lc¹

1970

Can. J. Microbiol.

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“…The nature and variation in amount of these compounds and especially of amino acids has attracted the attention of many workers. A large amount of information has been obtained about the nature, and amount of changes in the amino acid pool in mycelium of filamentous fungi at different stages of their life-cycle (Ritter, 1955 and Bent (1964) have made a detailed study on the changes of free and combined amino acids during the successive phases of spore germination, exponential growth and nitrogen starvation with several filamentous fungi. Nevertheless, very little information exists about the free sugars and of the free amino acids pools and their changes in the mycelium during the autolytic phase of growth.…”

Section: Introductionmentioning

confidence: 99%

Some Chemical Changes in the Mycelium of Aspergillus flavus during Autolysis

Loginova

Shishkina

Trotsenko

1966

Journal of General Microbiology

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SUMMARYSome chemical changes which took place in the mycelium of Aspergillus Jlavus during autolysis were studied. A . Jlavus underwent a ' neutral autolysis' in which a decrease of 73.9% in mycelial dry weight occurred. Glucose, xylose, maltose and mannitol were found in autolysing mycelium of A.Jlavus. The loss of free glucose amounted to 10 mg./flask during the whole period of autolysis. Xylose was decreased to half its initial content by the third day of autolysis and then disappeared by the ninth day, whereas maltose decreased at the beginning and then remained constant from the eighty-first to the hundred and eleventh day of incubation. Eighty-nine per cent of the initial amount of mannitol was lost during the first 9 days of autolysis, whereas fat seemed to be uninvolved in the process. Eighteen different amino acids were found in the autolysing mycelium of A . Jlavus. The general picture was of decreasing concentrations during autolysis, and amino acids decreased their content by about 95% with respect t o the concentration present at the beginning. This decrease partially accounted for the loss of mycelial-nitrogen observed during autolysis.

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Uptake of ¹⁴C‐glucose by Botryodiplodia theobromae Pat and changes in its carbohydrate content during growth

Olofinboba

1973

J of Basic Microbiology

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There is a rapid uptake of 14C-glucose and unlabelled glucose during the period of pigmentation of the mycelium of Botryodiplodia theobromae PAT. Incorporation of 14C-glucose into structural components of the mycelium started within 24 hours of incubation and progressively increased until the 14th day of growth.The mycelium contains glucose, isomaltose, and sucrose. There is a decrease in these carbohydrates during autolysis. Autolysis in B. theobromae occurs in an acid medium of p H 5.2.The growth of Botryodiplodia theobromae PAT, the causal organism of diseases of many economic tropical crops (ALASOADURA 1970) in culture can be divided into two distinct phases : Hyaline mycelium is produced during the first 3 -4 days of growth and this phase is followed by rapid pi gmentationof most of the mycelium. Microscopic examination of the mycelium during pigmentation shows a mixture of hyaline, thin hyphae, and pigmented stout hyphae. Observation of cross-section of wood of Antiaris africana stained blue by the fungus also reveals the two types of hyphae, with the food-storing parenchyma cells predominantly infected by the hyaline hyphae. OLOFINBOBA and LAWTON (1968) showed that the parenchyma cells of the wood of A . africana contain various carbohydrates which are rapidly utilized by the fungus. They suggested that the hyaline hyphae are nutritional in function. It was, however, not clear if rate of carbohydrate utilization was influenced by the pigmentation of some of the hyphae.Few reports (LAHOZ 1966) have appeared in recent years on the chemistry of autolysis of certain filamentous fungi. Most of the investigation was on the behaviour of nitrogenous materials present in the mycelium and the culture medium of such fungi (BENT 1964, BENT and MORTON 1964). LAHOZ et al. (1966) made the initial attempt to study the changes in carbohydrates during autolysis in Aspergillus flavus. Not so much has been reported about the changes in carbohydrates during growth and autolytic phases of fungi development.The present study is to investigate the influence of pigmentation on the utilization of glucose by B. theobromae. It is also designed to study the qualitative and quantitative changes in the carbohydrates of the mycelium of B. theobromae during growth and autolytic phases. Material and methodsUptake of 1%-glucose: The isolate used in this study was from blue-stained xylem of Antiaris africana. The culture medium consisted of the following composition (g/l distilled water): &(NO,), 0.5, KNO, 0.125, MgSO, x 7 H,O 0.125, KH,PO, 0.125. 5 ml of the 34*

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Effects of external factors on the amino acid pool of Penicillium griseofulvum

Cited by 16 publications

References 19 publications

Effect of tryptophan on alkaloid biosynthesis in cultures of a Claviceps species

Effect of tryptophan on alkaloid biosynthesis in cultures of a Claviceps species

Some Chemical Changes in the Mycelium of Aspergillus flavus during Autolysis

Uptake of ¹⁴C‐glucose by Botryodiplodia theobromae Pat and changes in its carbohydrate content during growth

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Effects of external factors on the amino acid pool of Penicillium griseofulvum

Cited by 16 publications

References 19 publications

Effect of tryptophan on alkaloid biosynthesis in cultures of a Claviceps species

Effect of tryptophan on alkaloid biosynthesis in cultures of a Claviceps species

Some Chemical Changes in the Mycelium of Aspergillus flavus during Autolysis

Uptake of 14C‐glucose by Botryodiplodia theobromae Pat and changes in its carbohydrate content during growth

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Uptake of ¹⁴C‐glucose by Botryodiplodia theobromae Pat and changes in its carbohydrate content during growth