Effects of extracellular calcium on cholesteatoma migration and adhesion in vitro☆☆☆★

Chroni c otitis media is hystopathologycaly defined as the presence of irreversible inflammatory tissue changes in the middle ear. Ossicular lesions represent the most prevalent change. Aim: to correlate the degree of ossicular chain changes seen during surgery with the inflammatory histological degree and the thickness of the cholesteatoma perimatrix. Study design: Cross-sectional study. Methods: Seventyone descriptions of surgeries done in patients submitted to tympanomastoydectomy were reviewed. Cholesteatoma were collected and fixed in 10% formaldehyde. Two slides were made for each cholesteatoma, one stained with HE and another with picrossirius. Images were obtained from light microscopy and digitally processed and "blindly" analyzed using Image Pro-Plus Software. For statistical analysis we used Spearman's coefficient. Differences were considered statistically significant if P≤0.05. Results: the ossicular chain was involved in 65 cases. The incus was the most frequently affected bone, followed by the stapes and the malleus. When the Spearman's coefficient was employed considering ossicular chain change degree with patient's age by the time of surgery, perimatrix thickness and histological degree of inflammation, correlations were not established. Conclusion: Our findings indicate that ossicular chain changes are practically universal when a cholesteatoma is present. We didn't find correlations related with bone erosion and cholesteatoma's histological findings.

Section: Introductionmentioning

confidence: 99%

Correlação da cadeia ossicular no transoperatório com achados histológicos de colesteatomas

Dornelles

Rosito²,

Meurer³

et al. 2007

“…If calcium is removed from cholesteatoma growth and migration stimulating culture media, there is a tenfold reduction in the degree of cholesteatoma migration. 15 Calcium is essential for cell to cell adhesion, for instance, between keratinocytes and substrate cells. These authors studied the in vitro inhibitory effects of all-trans retinoic acid on cholesteatoma migration in human cholesteatoma samples cultured in cell-growth simulating media.…”

Section: Discussionmentioning

confidence: 99%

Efeito do ácido trans-retinóico na inibição de colesteatoma em cobaias

Antunes

Fukuda

Penido

et al. 2008

Middle ear cholesteatoma affected more than 5 million people until the 80`s. Many animal models were used, unsuccessfully, to study an alternative therapy to cholesteatoma. Aim: observe the effect of the trans-retinoic acid in the inhibition of middle ear cholesteatomas induced by propylene glycol. Study Design: Clinical and Experimental. Methods: 25 guinea pigs were submitted to the application of a 100% propylene glycol solution in their bulla bilaterally and a solution of trans-retinoic acid was applied locally in the external right ear, while in the left ear saline solution was applied (control ear). The guinea pigs were slaughtered and their temporal bones were prepared for macroscopic and histological analysis. Results: The macroscopic findings had evidenced the presence of cholesteatoma in 25% of the right ears and 85% of the left ears (P=0.0003 *). The histological study had evidenced the presence of cholesteatoma in 30% of right ears and 75% of the left ears (P=0.0104*). Conclusion: The local use of the trans-retinoic acid is effective in inhibiting the induced formation of cholesteatomas in guinea pigs.

“…It is not properly demonstrated yet whether mineralized bone can be absorbed by collagenase. Other agents were incorporated, such as tumor necrosis factor (TNF), interleukins (IL-1 a ) and prostaglandins (PGE 2 ) [66][67][68] , to the hypothesis of bone absorption by biochemical action, exclusively played by collagenolytic enzymes.…”

Section: Power Of Bone Erosionmentioning

confidence: 99%

Algumas considerações sobre colesteatomas adquiridos pediátricos e adultos

Dornelles

Costa

Meurer

et al. 2005

Authors debate about cholesteatomas, from the first time this word was employed, by Muller, in 1838, until the recent updates. They dissert about its definition, etiology and pathology and present basic concepts about its biology. They also make a wide review about pediatric cholesteathoma, its epidemiology and biology, and compare it with adult cholesteatoma. Finally, they describe some articles about ossicle chain erosion and its correlation with cholesteatoma perimatrix, collagen and collagenase.