Effects of extraction time on total phenolic and flavonoid contents and antioxidant activities of a polyherbal drink

Hamad, Abdullah; Hartanti, Dwi

doi:10.1088/1755-1315/1200/1/012047

Cited by 3 publications

(1 citation statement)

References 40 publications

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“…Extraction time showed a significant impact at a linear level on the IC 50 of the extract's DPPH scavenging activity (p < 0.05). Earlier studies also signified the effect of extraction time on the increased antioxidant activity of extracts [39,40].…”

Section: Effects Of Operational Parameters On Tpc Extraction and Ic50...mentioning

confidence: 93%

Optimization and Characterization of Phenolic Extraction Conditions and Antioxidant Activity Evaluation of Adenanthera pavonina L. Bark

Begum,

Hossain,

Adnan

et al. 2023

Plants

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The presence of high levels of secondary metabolites in medicinal plants can significantly influence the progress of drug development. Here, we aimed to maximize phenolic extraction from Adenanthera pavonina L. stem bark using various solvents such as ethyl acetate, methanol, petroleum ether, and chloroform. A response surface method (RSM) with a central composite design (CCD) statistical technique was applied to optimize the extraction process, employing three important extracting parameters such as extraction time (h), temperature (°C), and solvent composition (% v/v of methanol/water) to obtain the highest phenolic content. Total phenolic content (TPC) and antioxidant activity (IC50 of extract’s DPPH radical scavenging activity) were used as response variables to find the influence of these extracting parameters. Among the various solvents used, methanol extract showed the highest contents of phenolics and the maximum level of antioxidant activity with a lower IC50 value. The notable TPC and IC50 value of the extract’s DPPH radical scavenging capacity were found to be 181.69 ± 0.20 mg GAE/g dry tissue and 60.13 ± 0.11 mg/mL, respectively, under the optimal conditions with a solvent composition of 71.61% (v/v) of methanol/water, extraction temperature of 42.52 °C, and extraction time of 24 h. The optimized extract of A. pavonina stem bark was further subjected to HPLC analysis, where six phenolic compounds, including coumarin, p-coumaric acid, chlorogenic acid, sinapic acid, gallic acid, and caffeic acid, were identified along with their respective quantities. Overall, the findings of this study uncover a low-cost analytical model for maximizing phenolic extraction from A. pavonina bark with enhanced antioxidant activity.

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Section: Effects Of Operational Parameters On Tpc Extraction and Ic50...mentioning

confidence: 93%

Optimization and Characterization of Phenolic Extraction Conditions and Antioxidant Activity Evaluation of Adenanthera pavonina L. Bark

Begum,

Hossain,

Adnan

et al. 2023

Plants

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Extraction Method and Crude Drug-to-Solvent Ratio Effects on the Antioxidant Properties and Physicochemical Profile during Storage of a Polyherbal Formulation Extract

Hartanti,

Insani,

Wahono

et al. 2024

BIO Web Conf.

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Java tea, seed-under-leaf, and turmeric are well-known for their antioxidant activity. The mixture with 40% Java tea leaves, 55% seed-under-leaf aerial parts, and 5% turmeric rhizomes produced a polyherbal formulation with a satisfactory antioxidant profile. This study aimed to develop the optimal extraction condition to produce water extract with good antioxidant properties that are stable during storage. The herbal mixture was extracted using Decoction A, Decoction B, and infusion methods at crude drug-tosolvent ratios of 1:10, 1:20, and 1:100. The antioxidant activity was evaluated by the standard 2,2-diphenyl1-picrylhydrazyl (DPPH) scavenging activity and ferric reducing antioxidant power (FRAP) assays. The total phenolic content (TPC) and total flavonoid content (TFC) were determined according to the standard methods. The extract from Decoction A at a 1:10 ratio was stored for 18 days at 7±3°C, and their physicochemical properties, i.e., color, pH, DPPH scavenging activity, TPC, and TFC, were evaluated accordingly. The extraction method and crude drug-to-solvent ratio significantly affected the extracts’ DPPH scavenging activity, FRAP, TPC, and TFC. Decoction A at a 1:10 ratio yielded extracts with the highest DPPH activity, TPC, and TFC and total flavonoid content, while Decoction B at a 1:20 ratio resulted in the highest FRAP. Extracts from Decoction A at a 1:10 ratio underwent chemical changes on day 6 of refrigerated storage. In conclusion, extraction by Decoction A at a crude drug-to-solvent ratio of 1:10 produced an antioxidant-rich extract that remained stable for six days during storage.

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Experimental Intensification and Modeling of Solid–Liquid Extraction of Polyphenols From Rice Husk Waste Using Organic Solvents

Abraham-Diego,

Reynel-Ávila,

Bonilla-Petriciolet

et al. 2024

International Journal of Chemical Engineering

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In the present study, the experimental intensification and modeling of the extraction process of antioxidants from rice husks using different organic solvents (ethanol, methanol, and acetone, and their mixtures with water) were analyzed and discussed. The best experimental extraction conditions to recover polyphenols from rice husks were identified, and the extraction kinetic data were modeled using the mechanistic and pseudo–second‐order models. The experimental results showed that the solvent‐based extraction of polyphenols from rice husks was endothermic where the ethanol and methanol offered the best separation performance. The polyphenol extraction process using ethanol and acetone was mainly influenced by the percentage of water contained in the solvent mixture used as separation medium, whereas the extraction temperature significantly affected the polyphenol extraction with methanol. The rice husk‐based extracts obtained with methanol and ethanol showed the highest total content of polyphenols (66.72 and 60.68 of gallic acid equivalent/g of biomass) and antioxidant capacity (71.93% and 72.02% 2,2‐diphenyl‐1‐picrylhydrazyl [DPPH] inhibition). The mechanistic model offered the best performance to describe the experimental polyphenol extraction, and its results were used to discuss and analyze the mass transfer parameters associated with the intraparticle diffusion of this extraction process. These results contribute to the valorization of rice husk residues as a potential feedstock to be incorporated in the biorefinery context for obtaining value‐added products.

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Effects of extraction time on total phenolic and flavonoid contents and antioxidant activities of a polyherbal drink

Cited by 3 publications

References 40 publications

Optimization and Characterization of Phenolic Extraction Conditions and Antioxidant Activity Evaluation of Adenanthera pavonina L. Bark

Optimization and Characterization of Phenolic Extraction Conditions and Antioxidant Activity Evaluation of Adenanthera pavonina L. Bark

Extraction Method and Crude Drug-to-Solvent Ratio Effects on the Antioxidant Properties and Physicochemical Profile during Storage of a Polyherbal Formulation Extract

Experimental Intensification and Modeling of Solid–Liquid Extraction of Polyphenols From Rice Husk Waste Using Organic Solvents

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