Effects of Fasting and Refeeding and Intravenous Glucose Infusion on In Vitro Lipogenesis in Bovine Adipose Tissue

Prior, Ronald L.; Jacobson, Juliet

doi:10.1093/jn/109.7.1279

Cited by 27 publications

(11 citation statements)

References 17 publications

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“…However, it is not clear whether these results can be extrapolated to lactating ruminants, particularly dairy cows, as glucose requirements Intravenous infusions of glucose stimulate key lipogenic enzymes in adipose tissue of dairy cows in a dose-dependent manner Mirja Carra ,* Bahaa Al-Trad ,* Gregory B. Penner , † Thomas Wittek , ‡ Gotthold Gäbel , § Manfred Fürll ,# 1 and glucose distribution may grossly differ between the lactating and the nonlactating state (Aschenbach et al, 2010). Furthermore, studies designed to investigate the effect of surplus glucose on lipogenesis in bovine adipose tissue (Prior and Jacobson, 1979;Prior and Scott, 1980;McLeod et al, 2007) have not included evaluating the dose effects of surplus glucose on the activities and gene expression levels of adipose lipogenic enzymes. Therefore, the first aim of the present study was to investigate the dose effect of an increasing intravenous glucose infusion on mRNA abundance and enzyme activity of the main subcutaneous adipose tissue (SAT) lipogenic enzymes involved in de novo FA synthesis in dairy cows.…”

Section: Introductionmentioning

confidence: 99%

Intravenous infusions of glucose stimulate key lipogenic enzymes in adipose tissue of dairy cows in a dose-dependent manner

Carra

Al‐Trad

Penner

et al. 2013

Journal of Dairy Science

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The present study was investigated whether increasing amounts of glucose supply have a stimulatory effect on the mRNA abundance and activity of key lipogenic enzymes in adipose tissue of midlactation dairy cows. Twelve Holstein-Friesian dairy cows in midlactation were cannulated in the jugular vein and infused with either a 40% glucose solution (n=6) or saline (n=6). For glucose infusion cows, the infusion dose increased by 1.25%/d relative to the initial net energy for lactation (NEL) requirement until a maximum dose equating to a surplus of 30% NEL was reached on d 24. This maximum dose was maintained until d 28 and stopped thereafter (between d 29-32). Cows in the saline infusion group received an equivalent volume of 0.9% saline solution. Samples of subcutaneous adipose tissue were taken on d 0, 8, 16, 24, and 32 when surplus glucose reached 0, 10, 20, and 30% of the NEL requirement, respectively. The mRNA abundance of fatty acid synthase, cytoplasmic acetyl-coenzyme A synthetase, cytoplasmic glycerol 3-phosphate dehydrogenase-1, and glucose 6-phosphate dehydrogenase showed linear treatment × dose interactions with increasing mRNA abundance with increasing glucose dose. The increased mRNA abundance was paralleled by a linear treatment × dose interaction for fatty acid synthase and acetyl-coenzyme A synthetase enzymatic activities. The mRNA abundance of ATP-citrate lyase showed a tendency for linear treatment × dose interaction with increasing mRNA abundance with increasing glucose dose. The mRNA abundance of all tested enzymes, as well as the activities of fatty acid synthase and acetyl-coenzyme A synthetase, correlated with plasma glucose and serum insulin levels. In a multiple regression model, the predictive value of insulin was dominant over that of glucose. In conclusion, gradual increases in glucose supply upregulate key lipogenic enzymes in adipose tissue of midlactating dairy cows with linear dose dependency. Insulin appears to be critically involved in this regulation.

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Section: Introductionmentioning

confidence: 99%

Intravenous infusions of glucose stimulate key lipogenic enzymes in adipose tissue of dairy cows in a dose-dependent manner

Carra

Al‐Trad

Penner

et al. 2013

Journal of Dairy Science

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“…The re-establishment of glycemia may well be attributed to the decreased use of blood glucose by the heart (Daniel, Love and Pratt, 1978). the digestive tract (Hanson and Parsons, 1978 ;Prior and Jacobson, 1979) and the adipose tissue (Olefsky, 1976).…”

Section: Introductionmentioning

confidence: 99%

Influence of diet and prolonged fasting on blood lipids, ketone bodies, glucose and insulin in adult sheep

Bouchat¹,

Doizé²,

Paquay³

et al. 1981

Reprod. Nutr. Dévelop.

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Summary. In order to study changes in blood composition during feeding and prolonged fasting, six adult Suffolk ewes received three different diets during three periods of four weeks. Two intake levels (one and three times maintenance) and two roughage/concentrate ratios (100 and 14 p. 100 roughage) were compared according to a twofold latin square. Afterwards the animals were fasted for an 11-day period. The influence of previous dietary treatment on changes in blood composition during fasting clearly depended on the body conformation of the animal.When animals showed similar body conformation, no differences in the blood changes were observed during fasting. But when lean and fat animals were compared, extensive differences were found in the blood composition after the food was removed.Introduction.

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“…Activity of the enzyme is decreased in ovine adipose tissue by fasting, but decreases are not ob served until after 48 hours of fasting while decreases in fatty acid synthesis from acetate are observed within 24 hours (Ingle et al, 1973). This observation by Ingle et al (1973) (Prior and Jacobson, 1979a). Prior and Jacobson (1979b) found that pyruvate could be uti lized at greater rates than acetate in the absence of glucose in vitro by bovine adipose tissue; Prior and Jacobson (1979c) have reported greater rates of utilization of pyruvate than glucose for fatty acid synthesis by bovine adipose tissue.…”

Section: A Generation Of Cytosolic Acetyl-coamentioning

confidence: 92%

“…Concentrations of specific isozymes of lactate dehydrogenase 54 (EC 1.1.1.27) suggest that lactate would be converted to pyruvate (Prior and Jacobson, 1979a). Furthermore, both pyru vate and lactate are decarboxylated before being converted to fatty acids (Prior and Jacobson, 1979b).…”

Section: "^Observed Value Is Significantly Different From Required (Pmentioning

confidence: 99%

Lipogenesis in adipose tissue from cattle

Whitehurst

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Effects of Fasting and Refeeding and Intravenous Glucose Infusion on In Vitro Lipogenesis in Bovine Adipose Tissue

Cited by 27 publications

References 17 publications

Intravenous infusions of glucose stimulate key lipogenic enzymes in adipose tissue of dairy cows in a dose-dependent manner

Intravenous infusions of glucose stimulate key lipogenic enzymes in adipose tissue of dairy cows in a dose-dependent manner

Influence of diet and prolonged fasting on blood lipids, ketone bodies, glucose and insulin in adult sheep

Lipogenesis in adipose tissue from cattle

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