Effects of fatty acid transport protein 1 on proliferation and differentiation of porcine intramuscular preadipocytes

Liu

Molecular Nutrition Food Res

et al. 2021

Introduction As an exogenous food contaminant, dietary oxidized lipid impairs growth and development, and triggers chronic diseases in humans or animals. This study explores the effects of soybean oil with different oxidative degree on the placental injury of gestational rats. Methods and Results Thirty‐two female adult rats are randomly assigned to four groups. The control group is fed the purified diet with fresh soybean oil (FSO), and the treatment groups are fed purified diets with lipid content replaced by oxidized soybean oil (OSO) at 200, 400, and 800 mEqO2 kg−1 from conception until delivery. On day 20 of gestation, OSO decreased placental and embryonic weights as the oxidative degree increased linearly and quadratically. The expression of Bax showed a linear increase, and Bcl‐2 decreased as the oxidative degree increased. The expression of Fosl1 and Esx1 is linearly and quadratically decreased in OSO‐treated groups than FSO group. OSO decreased the level of IL‐10 but increased expression of IL‐1β in placenta and plasma. OSO remarkably upregulates levels of Fatp1 and Glut1 and decreases expression of Snat2 and Glut3. Conclusion OSO aggravates placental injury by modulating nutrient transporters and apoptosis‐related genes, impedes placental growth and development, and ultimately leads to the decrease of fetal weight.

Section: Discussionmentioning

confidence: 99%

Maternal Exposure to Oxidized Soybean Oil Impairs Placental Development by Modulating Nutrient Transporters in a Rat Model

Liu

Molecular Nutrition Food Res

et al. 2021

“…It was reported that preadipocyte differentiation is a key factor affecting IMF deposition [ 36 ]. However, to date, only a few studies have identified genes that play important roles in porcine intramuscular preadipocyte differentiation, such as FABP1 [ 37 ], FABP4 , GPR39 [ 7 ], and CD36 [ 38 ]. The mechanisms by which intramuscular preadipocyte differentiation and fat deposition are regulated have also remained unclear in chicken.…”

Section: Discussionmentioning

confidence: 99%

Analysis of long noncoding RNA and mRNA using RNA sequencing during the differentiation of intramuscular preadipocytes in chicken

Zhang

Han

et al. 2017

PLoS ONE

Long noncoding RNAs (lncRNAs) regulate metabolic tissue development and function, including adipogenesis. However, little is known about the function and profile of lncRNAs in intramuscular preadipocyte differentiation in chicken. Here, we identified lncRNAs in chicken intramuscular preadipocytes at different differentiation stages using RNA sequencing. A total of 1,311,382,604 clean reads and 25,435 lncRNAs were obtained from 12 samples. In total, 7,433 differentially expressed genes (4,698 lncRNAs and 2,735 mRNAs) were identified by pairwise comparison. These 7,433 differentially expressed genes were grouped into 11 clusters based on their expression patterns by K-means clustering. Using Weighted Gene Coexpression Network Analysis, we identified four stage-specific modules positively related to I0, I2, I4, and I6 stages and two stage-specific modules negatively related to I0 and I2 stages, respectively. Many well-known and novel pathways associated with intramuscular preadipocyte differentiation were identified. We also identified hub genes in each stage-specific module and visualized them in Cytoscape. Our analysis revealed many highly-connected genes, including XLOC_058593, BMP3, MYOD1, and LAMP3. This study provides a valuable resource for chicken lncRNA study and improves our understanding of the biology of preadipocyte differentiation in chicken.

“…A positive correlation between FATP1 and intramuscular fat deposition has been reported in pig [9]. Moreover, overexpression of FATP1 induced intramuscular preadipocyte proliferation and fat accumulation in pig [10]. FATP1 overexpression also significantly upregulated the expression levels of PPARγ, CEBPα, LPL, PLIN1, and FASN, and downregulated the expression of β-catenin.…”

Section: Introductionmentioning

confidence: 80%

“…FATP1 overexpression also significantly upregulated the expression levels of PPARγ, CEBPα, LPL, PLIN1, and FASN, and downregulated the expression of β-catenin. It was subsequently hypothesized that FATP1 might repress the Wnt,/β-catenin signaling pathway and stimulate adipogenesis in porcine intramuscular preadipocytes [10]. This hypothesis is supported by the results of RNA interference experiments in chicken.…”

Section: Introductionmentioning

confidence: 90%

“…Preadipocyte differentiation into mature adipocytes is also known to involve additional marker genes. To further investigate preadipocyte differentiation, the expression levels of PPARG, a marker gene of adipogenic differentiation [9], and LPL, a marker gene of lipid metabolism [10,11], were analyzed by qRT-PCR. The results reveal that overexpression of FATP1 up-regulated the expression of these two genes at the 4th day of differentiation (Figure 2C).…”

Section: Overexpression Of Fatp1 Promotes Preadipocyte Differentiation In Qinchuan Cattlementioning

confidence: 99%

See 1 more Smart Citation

The Effect of FATP1 on Adipocyte Differentiation in Qinchuan Beef Cattle

Liu

et al. 2021

Animals

FATP1 plays an important role in the regulation of fatty acid metabolism and lipid accumulation. In this study, we investigated the patterns of FATP1 expression in various tissues obtained from calf and adult Qinchuan cattle, and in differentiating adipocytes. Next, we investigated the effect of FATP1 expression on preadipocyte differentiation in Qinchuan cattle using overexpression and interference assays. We also identified the differentially expressed genes (DEGs) and pathways associated with FATP1 overexpression/interference. Our results reveal that FATP1 was broadly expressed in heart, kidney, muscle, small intestine, large intestine, and perirenal fat tissues. While FATP1 overexpression promoted preadipocyte differentiation, fat deposition, and the expression of several genes involved in fat metabolism, FATP1 interference had the opposite effects on adipocyte differentiation. Following FATP1 overexpression and FATP1 interference in adipocytes, RNA-seq analysis was performed to identify DEGs related to fat metabolism. The DEGs identified include SLPI, STC1, SEMA6A, TNFRSF19, SLN, PTGS2, ADCYP1, FADS2, and SCD. Pathway analysis revealed that the DEGs were enriched in the PPAR signaling pathway, AMPK signal pathway, and Insulin signaling pathway. Our results provide an in-depth understanding of the function and regulation mechanism of FAPT1 in fat metabolism.