The aim of our study is to find out the influence of different share of cereals and various fertilisation on the grain yield and quality of winter wheat. The long-term field trial with 40, 60 and 80% share of the cereals and two levels of fertilisation (H1 mineral fertilisation + organic manure Veget®; H2 mineral fertilisation only) were carried out in the very warm and dry area of continental weather on luvi-haplic chernozem. In the years 2010–2013, the grain yield, the wet gluten content, gluten index, the falling number and sedimentation index of winter wheat according to Zeleny were investigated. The significantly higher grain yield of winter wheat was recorded after preceding crop of common pea. The yield of cereals in crop rotation with 60% share of cereals (7.00 t/ha) was significantly higher than in crop rotation with 80% share of cereals (6.78 t/ha).The statistically higher wet gluten content after pea fore-crop was found out when the mineral fertilisation and organic fertiliser Veget® were applied (33.4%) with comparison to the treatment with mineral fertilisation only (30.08%).