Effects of feruloyl esterase, non-starch polysaccharide degrading enzymes, phytase, and their combinations on in vitro degradation of rice bran and nutrient digestibility of rice bran based diets in adult cockerels

Liu, Q.; Zhou, Dingyong; Chen, L.; Zhuang, Songlin

doi:10.1016/j.livsci.2015.06.007

Cited by 14 publications

(4 citation statements)

References 33 publications

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“…To the best of our knowledge, both in vitro and in vivo digestion studies with rice grain and NSPases are limited, and the results of in vivo digestibility experiments with NSPases are variable 33–35 . Rice AX is more complex than wheat, with a high degree of double substitutions and greater amount of p ‐coumaric acid 8 .…”

Section: Discussionmentioning

confidence: 99%

Improving the digestibility of cereal fractions of wheat, maize, and rice by a carbohydrase complex rich in xylanases and arabinofuranosidases: an in vitro digestion study

et al. 2020

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BACKGROUND Cereal co‐products rich in dietary fibres are increasingly used in animal feed. The high fibre content decreases the digestibility and reduces the nutrient and energy availability, resulting in lower nutritive value. Therefore, this study investigated the ability of two carbohydrase complexes to solubilize cell‐wall polysaccharides, in particular arabinoxylan (AX), from different cereal fractions of wheat, maize, and rice using an in vitro digestion model of the pig gastric and small intestinal digestive system. The first complex (NSPase 1) was rich in cell‐wall‐degrading enzymes, whereas the second complex (NSPase 2) was additionally enriched with xylanases and arabinofuranosidases. The extent of solubilization of insoluble cell‐wall polysaccharides after in vitro digestion was evaluated with gas–liquid chromatography and an enzymatic fingerprint of the AX oligosaccharides was obtained with high‐performance anion‐exchange chromatography with pulsed amperometric detection. RESULTS The addition of carbohydrase increased the digestibility of dry matter and solubilized AX in particular, with the greatest effect in wheat fractions and less effect in maize and rice fractions. The solubilization of AX (expressed as xylose release) ranged from 6% to 41%, and there was an increased effect when enriching with xylanases and arabinofuranosidases in wheat aleurone and bran of 19% and 14% respectively. The enzymatic fingerprint of AX oligosaccharides revealed several non‐final hydrolysis products of the enzymes applied, indicating that the hydrolysis of AX was not completed during in vitro digestion. CONCLUSION These results indicate that the addition of a carbohydrase complex can introduce structural alterations under in vitro digestion conditions, and that enrichment with additional xylanases and arabinofuranosidases can boost this effect in wheat, maize, and rice. © 2020 Society of Chemical Industry

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Section: Discussionmentioning

confidence: 99%

Improving the digestibility of cereal fractions of wheat, maize, and rice by a carbohydrase complex rich in xylanases and arabinofuranosidases: an in vitro digestion study

et al. 2020

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“…However, the quantification of phenolic acid release additionally supported this. Liu et al 26 reported that NSPase released ferulic acid from the rice bran cell wall matrix during in vitro digestion and suggested that the disruption of the matrix might expose free or weakly bound ferulic acids from the inside. Although we did not encounter much effect directly on the insoluble NSP and AX in rice bran, these observations indicate that some redistributions within the matrix were indeed realized.…”

Section: ■ Discussionmentioning

confidence: 99%

Carbohydrase Complexes Rich in Xylanases and Arabinofuranosidases Affect the Autofluorescence Signal and Liberate Phenolic Acids from the Cell Wall Matrix in Wheat, Maize, and Rice Bran: An In Vitro Digestion Study

Vangsøe

Nørskov

Devaux

et al. 2020

J. Agric. Food Chem.

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The high fiber content of cereal coproducts used in animal feed reduces the digestibility and nutrient availability. Therefore, the aim of this study was to elucidate the ability of two carbohydrase complexes to degrade the cell wall of wheat, maize, and rice during in vitro digestion. One complex was rich in cell-wall-degrading enzymes (NSPase 1), and the other was similar but additionally enriched with xylanases and arabinofuranosidases (NSPase 2). Degradation of arabinoxylan, the main cereal cell wall polysaccharide, was followed directly by gas–liquid chromatography (GLC) and indirectly through phenolic acid liberation as quantified by liquid chromatography–tandem mass spectrometry (LC–MS/MS). The effect was additionally visualized using a unique multispectral autofluorescence approach. Wheat fractions, in particular aleurone, were susceptible to degradation as judged from the redistribution of arabinoxylan (25% reduction in insoluble arabinoxylan), whereas the highest relative liberation of ferulic acid was observed in rice bran (6%). All cereal fractions, except for maize, had a higher release of ferulic acid with NSPase 2 than NSPase 1 (38% in rice and wheat bran, 30% in wheat whole grain, and 28% in wheat aleurone). Thus, the carbohydrase complexes were able to degrade important cell wall components during in vitro digestion but apparently through different mechanisms in wheat, maize, and rice.

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“…(2015)Multi-glycanaseWheat and barley180 unit/gImprove growth rate and carcass traits, blood parameters and gut physicochemical properties of broiler chickens.Kalantar et al. (2015)XylanaseDe-oiled rice bran10 g/100 kg feedBreast muscle, thigh muscle and giblet weight (percentage) was significantly increased.Anuradha and Roy (2015)Xylanase, cellulase and β-galactosidaseRice bran4520 U, 4060 U, and 2700 UEnhance cell wall hydrolysis of rice bran with increased nutrient digestibility.Liu et al. (2015)Xylanase, glucanase, mannanaseWheat-soybean meal500 mg/kg dietEnzyme supplementation increase body weight, decrease digesta viscosity, lowered ileal C. perfringens and Lactobacillus colonization.Sun et al.…”

Section: Main Textmentioning

confidence: 99%

An update on carbohydrases: growth performance and intestinal health of poultry

Raza

Bashir

Tabassum

2019

Heliyon

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Poultry is an imperative domesticated livestock species that provides high quality protein and micronutrients as meat and eggs. In poultry production, feed is the single major input constituting 70–75% of total production cost. Feed mainly consists of cereal grains, those provide energy to the birds. However, these grains contain different levels of anti-nutritional factors such as non-starch polysaccharides (NSP). These NSP are indigestible by poultry birds due to the lack of vital endogenous enzymes (carbohydrases) thus increase intestinal viscosity which slower the migration and absorption of nutrients. Consequently, these NSP may also increase the chances for infection by inducing competition within gut microbiota for digestible nutrients. This affects bird's health and increases the production cost. Therefore, there is a need to find efficient and effective solutions for these problems. Carbohydrases supplementation have an important role in poultry diets with high NSP contents. Feed enzymes are being used from years to enhance growth performance and digestibility but have limited activity for selective ingredients. New generation carbohydrases with a board range of activity and stability help to degrade the complex substrates and improve growth performance of poultry. Present review summarizes the updated literature on the use of carbohydrases to improve bird's performance and intestinal health.

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Effects of feruloyl esterase, non-starch polysaccharide degrading enzymes, phytase, and their combinations on in vitro degradation of rice bran and nutrient digestibility of rice bran based diets in adult cockerels

Cited by 14 publications

References 33 publications

Improving the digestibility of cereal fractions of wheat, maize, and rice by a carbohydrase complex rich in xylanases and arabinofuranosidases: an in vitro digestion study

Improving the digestibility of cereal fractions of wheat, maize, and rice by a carbohydrase complex rich in xylanases and arabinofuranosidases: an in vitro digestion study

Carbohydrase Complexes Rich in Xylanases and Arabinofuranosidases Affect the Autofluorescence Signal and Liberate Phenolic Acids from the Cell Wall Matrix in Wheat, Maize, and Rice Bran: An In Vitro Digestion Study

An update on carbohydrases: growth performance and intestinal health of poultry

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