Effects of fibrillation on the wood fibers’ enzymatic hydrolysis enhanced by mechanical refining

Liu, Wei; Wang, Bing; Hou, Qingxi; Chen, Wei; Wu, Ming

doi:10.1016/j.biortech.2016.01.074

Cited by 28 publications

(19 citation statements)

References 22 publications

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“…2 c). Similar reduction in particle size with the increase of refining intensity, when PFI and disc refining is applied in pretreated biomass, has been previously observed [ 7 , 8 , 10 – 12 , 14 ]. Reduction in particle size is believed to improve enzymatic hydrolysis due to the associate increase in surface area.…”

Section: Resultssupporting

confidence: 80%

“…Therefore, it can be concluded that mechanical refining is improving the water uptake of fibers within the cell wall and on the fiber surface. Many authors have reported a direct relationship between refining intensity and WRV for pretreated biomasses [ 9 , 10 , 12 , 15 ]. A strong correlation between WRV and enzymatic hydrolysis is usually observed.…”

Section: Resultsmentioning

confidence: 99%

“…Water retention values, dye adsorption and differential scanning calorimetry were used to access the effect of mechanical refining on biomass surface area. Refining processes increased cell wall porosity and surface area, which contributed to increase the exposure of carbohydrates to cellulolytic enzymes [ 9 , 10 , 12 , 13 , 15 , 16 ]. The effect of mechanical refining on cellulose crystallinity was also evaluated.…”

Section: Introductionmentioning

confidence: 99%

“…A reduction of crystalline regions was observed via X-ray diffraction as a result of the compression and shear forces experienced by the fiber cell wall during mechanical refining. The reduction of crystallinity was believed to increase biomass digestibility [ 12 , 17 ].…”

Section: Introductionmentioning

confidence: 99%

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Toward an understanding of the increase in enzymatic hydrolysis by mechanical refining

Assis

Huang

Driemeier

et al. 2018

Biotechnol Biofuels

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BackgroundMechanical refining is a low-capital and well-established technology used in pulp and paper industry to improve fiber bonding for product strength. Refining can also be applied in a biorefinery context to overcome the recalcitrance of pretreated biomass by opening up the biomass structure and modifying substrate properties (e.g., morphology, particle size, porosity, crystallinity), which increases enzyme accessibility to substrate and improves carbohydrate conversion. Although several characterization methods have been used to identify the changes in substrate properties, there is no systematic approach to evaluate the extent of fiber cell wall disruption and what physical properties can explain the improvement in enzymatic digestibility when pretreated lignocellulosic biomass is mechanically refined. This is because the fiber cell wall is complex across multiple scales, including the molecular scale, nano- and meso-scale (microfibril), and microscale (tissue level). A combination of advanced characterization tools is used in this study to better understand the effect of mechanical refining on the meso-scale microfibril assembly and the relationship between those meso-scale modifications and enzymatic hydrolysis.ResultsEnzymatic conversion of autohydrolysis sugarcane bagasse was improved from 69.6 to 77.2% (11% relative increase) after applying mechanical refining and an increase in enzymatic digestibility is observed with an increase in refining intensity. Based on a combination of advanced characterizations employed in this study, it was found that the refining action caused fiber size reduction, internal delamination, and increase in pores and swellability.ConclusionsA higher level of delamination and higher increase in porosity, analyzed by TEM and DSC, were clearly demonstrated, which explain the faster digestibility rate during the first 72 h of enzymatic hydrolysis for disc-refined samples when compared to the PFI-refined samples. In addition, an increased inter-fibrillar distance between cellulose microfibrils at the nano–meso-scale was also revealed by SFG analysis, while no evidence was found for a change in crystalline structure by XRD and solid-state NMR analysis.

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Section: Resultssupporting

confidence: 80%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Toward an understanding of the increase in enzymatic hydrolysis by mechanical refining

Assis

Huang

Driemeier

et al. 2018

Biotechnol Biofuels

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“…During processing of composites in the twin‐screw extruder, shear causes fibrillation and reduction of the aspect ratio of the fibers . External fibrillation of the fibers probably occurs, which is defined as a peeling off of nanofibril bundles from the fiber surface, and this can occur as a chemical or mechanical process. In this work, fibrillation of CF was done by HIUS to avoid aspect ratio reduction; see Figure .…”

Section: Resultsmentioning

confidence: 99%

Polypropylene composite reinforced with fibrillated curaua fiber and using maleic anhydride as coupling agent

Nacas

Silva

Paoli

et al. 2017

J of Applied Polymer Sci

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Curaua fibers (CF) were fibrillated using high-intensity ultrasonication, which is fast and uses water as a solvent. The fibrillated fibers (CF f ) were used as reinforcement (20 wt %) in polypropylene composites processed by extrusion with or without 2 wt % of polypropylene grafted with maleic anhydride (PPMA). Fibrillation promotes an increase from 11.3 to 33.8 in the aspect ratio of the fiber. Sonication caused extraction of lignin and a decrease in the degree of crystallinity of the CF. Extrusion of the composites causes no additional fibrillation of the CF. A composite reinforced with CF f had tensile and flexural strength of 24.9% and 51.5%, respectively, higher than that of pristine PP. The use of PPMA and CF f in the composite promotes a further increase of 30% and 50.5% in these parameters, respectively. A thermal analysis of the composites using CF f with or without PPMA showed similar behavior.

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Fiber Modification

Bajpai¹

2018

Biotechnology for Pulp and Paper Processing

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Effects of fibrillation on the wood fibers’ enzymatic hydrolysis enhanced by mechanical refining

Cited by 28 publications

References 22 publications

Toward an understanding of the increase in enzymatic hydrolysis by mechanical refining

Toward an understanding of the increase in enzymatic hydrolysis by mechanical refining

Polypropylene composite reinforced with fibrillated curaua fiber and using maleic anhydride as coupling agent

Fiber Modification

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