Effects of fish CYP inducers on difloxacin N-demethylation in kidney cell of Chinese idle (Ctenopharyngodon idellus)

Yu, Lu; Yang, Xian Le; Wang, Xiang Ling; Wen, Yu; Hu, Kun

doi:10.1007/s10695-009-9342-6

Cited by 6 publications

(3 citation statements)

References 51 publications

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“…, 2008). Currently, research involving the effects of drugs on CYPs, and CYPs‐mediated drug–drug interaction received growing attraction in various economical animals, including fishes (Yu et al. , 2009; Yu & Yang, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Thus, when the inducer or inhibitor of CYPs was co-administrated with the substrate drugs (the substrates of CYPs), the adverse effects might come out (Hasselberg et al, 2005(Hasselberg et al, , 2008Yamauchi et al, 2008). Currently, research involving the effects of drugs on CYPs, and CYPs-mediated drug-drug interaction received growing attraction in various economical animals, including fishes (Yu et al, 2009;Yu & Yang, 2010).…”

Section: Introductionmentioning

confidence: 99%

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Effects of enrofloxacin on cytochromes P4501A and P4503A in Carassius auratus gibelio (crucian carp)

Li²,

Sun³

et al. 2011

Vet Pharm & Therapeutics

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Currently, although enrofloxacin (EF) as a widely used veterinary medicine has begun to apply to treating fish bacterial infections, the researches on the effects of EF on their main drug metabolic enzymes are limited. To investigate the effects of EF on fish cytochromes P450 (CYPs) 1A and 3A, the enzymatic activities and expressions (mRNA and protein) of crucian carp CYP1A and CYP3A after EF administration were examined. For CYP1A, in the in vivo experiments, EF exhibited potent inhibition on the CYP1A-related ethoxyresorufin-O-deethylase (EROD) activity, as well as CYP1A expressions at both protein and mRNA levels, at 24 h after administration with different EF dosages (3, 10, 30, and 60 mg/kg); Furthermore, CYP1A enzymatic activity and expressions at both protein and mRNA levels decreased more with increasing EF dosages. Additionally, the in vitro experimental results showed that, after incubated with microsomes, EF did not change the EROD activity through interacting directly with CYP1A. For CYP3A, the in vitro and in vivo experimental results demonstrated that EF could inhibit the CYP3A-related erythromycin N-demethylase activity in a time- and dose-dependent manner, while it did not suppress CYP3A expressions at both protein and mRNA levels after administration with EF for a short period (no more than 24 h); however, after injection with EF at a high dose (10 mg/kg) for a long period, the CYP3A protein and mRNA reached their lowest levels at 96 and 48 h, respectively. These results indicate that EF can suppress CYP1A expressions in a dose-dependent manner, thereby inhibiting further its catalytic activity; meanwhile, both the interactions of EF with CYP3A and the expressions decrease (protein and mRNA) caused by EF contribute to the CYP3A inhibition.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of enrofloxacin on cytochromes P4501A and P4503A in Carassius auratus gibelio (crucian carp)

Li²,

Sun³

et al. 2011

Vet Pharm & Therapeutics

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show abstract

“…The first indications that CYP1A were involved in the metabolism of oxolinic acid were described by Ishida (1992), who treated carp (Cyprinus carpio) with polychlorinated biphenyl, a CYP1A inducer, before the treatment with oxolinic acid. CYP1A was also responsible for difloxacin (DIF) metabolism in crucian carp liver (Fu, Yang, Zhang, Yu & Hu 2011) and its N-demethylation in Chinese idle (Ctenopharyngodon idellus) kidney (Yu & Yang 2009;Yu, Yang, Wang, Yu & Hu 2010). After DIF treatment, CYP1A expression gradually decreased and was significantly lower than the control.…”

Section: Interactions With Fluoroquinolonesmentioning

confidence: 99%