Effects of fishmeal replacement by Clostridium autoethanogenum protein on the growth, digestibility, serum free amino acid and gene expression related to protein metabolism of obscure pufferfish (Takifugu obscurus)

Cui, Xishuai; Ma, Qiang; Duan, Mei; Xu, Houguo; Liang, Mengqing; Wei, Yuliang

doi:10.1016/j.anifeedsci.2022.115445

Cited by 13 publications

(6 citation statements)

References 44 publications

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“…In large yellow croaker, FM replacement with CAP by up to 100% induced almost linear reductions in muscle hardness, collagen content, and myofiber density, while a reverse pattern was observed in myofiber diameter 87 . Similar observations of reduction in flesh firmness by dietary FM substitution with CAP were reported in largemouth bass ( Micropterus salmoides ) and obscure pufferfish ( Takifugu obscurus ) 88,89 . In a recent study, dietary CAP replacing 30% and 100% of soybean meal significantly increased flesh cohesiveness, springiness, gumminess, and chewiness in grass carp, while shear force decreased 90 .…”

Section: Strategies To Regulate Texture Characteristics In Aquaculturesupporting

confidence: 58%

The flesh texture of teleost fish: Characteristics and interventional strategies

Wang,

Qiao,

Zhang

et al. 2023

Reviews in Aquaculture

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With the growing demand of consumers for high‐quality aquatic products, controlling the harvest quality of farmed fish is becoming increasingly important in aquaculture industry. The texture is a sensory property that covers a group of attributes, such as firmness, tenderness, chewiness, adhesiveness, resilience, etc., which greatly determine consumers' perception and satisfaction for flesh products. Regarding texture quality, extensive research has been conducted in recent decades based on its related theories, influencing factors, and intervention strategies. However, the information available is largely scattered and disjointed. Essentially, flesh texture is mainly determined by the collective contribution of three major muscle components: muscle fibres, intramuscular connective tissue, and intramuscular fat. Therefore, changing these components in living animals through nutritional and management factors has the potential to modulate texture quality. Here, we briefly introduced the features and development of the three muscle components mentioned above, particularly their potential contributions to texture quality of fish flesh. Exciting promises to manipulate flesh texture through dietary intervention, feed restriction, and exercise training were also discussed. To ensure their applications in large‐scale aquaculture facilities, specific considerations of these regimens should be incorporated in experimental design parameters, including the species and age of fish, research system, experiment duration, and exercise intensity. Finally, the existing challenges and limitations of fish texture studies were presented, along with some viewpoints about current work and future directions of this field.

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Section: Strategies To Regulate Texture Characteristics In Aquaculturesupporting

confidence: 58%

The flesh texture of teleost fish: Characteristics and interventional strategies

Wang,

Qiao,

Zhang

et al. 2023

Reviews in Aquaculture

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“…Gene expression analysis were conducted as our previous studies [ 17 ]. In briefl, total RNA were isolated from intestine and brain by using RNAiso Plus kit (Takara, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…In 2021, according to China Fishery Statistical Yearbook (2022), the production of pufferfish was up to 29,940 tons. In particular, obscure pufferfish ( Takifugu obscurus ) and tiger puffer ( Takifugu rubripes ) had become the most important cultured species, because they were the only two pufferfish that the Chinese government conditionally allows to be processed and operated [ 17 , 18 ]. However, obscure pufferfish and tiger puffer had sharp teeth and often cannibalize each other, which were considered as a typical cannibalistic species [ 19 ].…”

Section: Introductionmentioning

confidence: 99%

Growth, Cannibalism, and 5-TH Metabolism in Pufferfish (Takifugu obscurus ♀ × Takifugu rubripes): The Role of Graded Levels of Dietary Tryptophan

Wei,

Cui,

Zhou

et al. 2023

Aquaculture Nutrition

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The objective of this study was to investigate the potential effect of graded levels of tryptophan on the growth, cannibalism, and 5-hydroxytryptpamine (5-TH) metabolism in pufferfish (Takifugu obscurus ♀ × Takifugu rubripes ♂). A 63-day feeding trial was performed wherein pufferfish were fed four diets. Three experimental diets were formulated with various levels of tryptophan based on the control diet. Four diets were named as T1, T2, T3, and T4, corresponding to 4.30, 7.80, 14.90, and 23.70 g kg−1 tryptophan of dry diet. Final body weight, weight gain, and specific growth rate were similar between the T1 and T4 groups, but exhibited a significantly increased trend compared to the T2 group. Although survival rate was not affected by various levels of dietary tryptophan, intraspecific cannibalism was significantly reduced in the group fed with highest level of tryptophan (T4). For free amino acid in brain, the concentration of tryptophan was the highest in the T3 group and the lowest in the T2 group, while phenylalanine, tyrosine, and methionine showed an opposite trend between those two groups. The levels of dietary tryptophan not only affected the expression of aromatic amino acid transporter TAT1, but also affected the expression of B0AT1, B0AT2, and 4F2hc in intestine, as well as B0AT1, y+LAT1, and LAT2 in brain. The activity of tryptophan hydroxylase (TPH) in serum increased with the increase of dietary tryptophan, and the expression of TPH1 in brain upregulated in the excessive tryptophan groups (T2, T3, and T4). MAO activity in serum as well as its gene expression in brain and intestine showed a decreased trend in the T4 group. In conclusion, excessive tryptophan (23.70 g kg−1 of dry diet, corresponding to 50.3 g kg−1 of dietary protein) in feed could mitigate cannibalistic behavior of pufferfish and promote the growth, and the reason for this effect might affect the metabolism of 5-TH in vivo.

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“…Moreover, no toxic genes found in the complete genome sequence of C. autoethanogenum indicated that CAP has low risk potential (Norman et al 2018). Recently, CAP was proved to successfully replace certain amount of shmeal in the feed of black sea bream Acanthopagrus schlegelii (Chen et al 2020), largemouth bass Micropterus salmoides (Lu et (Huang et al 2023), puffer sh Takifugu obscurus (Cui et al 2022) or soybean meal in the feed of grass carp Ctenopharygodon Idella (Fan et al 2022;Xue et al 2023), Jian carp Cyprinus carpio var. Jian (Li et al 2021) and tilapia Oreochromis niloticus (Maulu et al 2021a, b).…”

Section: Introductionmentioning

confidence: 99%

Effects of Clostridium autoethanogenum protein on growth performance, intestine and liver health of turbot Scophthalmus maximus during grow-out phase

Bai

Pan

et al. 2023

Preprint

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Clostridium autoethanogenum protein (CAP) is an emerging protein source with several key advantages and it has investigated on juvenile turbot (9.13 g). However, it was indicated that fish growth stage is a decisive factor for CAP performance and the influence of CAP on turbot intestine health has not been reported. Thus, it is worth investigating the effects of CAP on turbot extensively. In the present study, a 67-day feeding experiment was conducted to evaluate the effects of CAP on turbot in the grow-out phase from growth performance, intestine and liver health. Six hundred and thirty turbot with an initial body weight of about 150 g were randomly divided into 6 groups, which were fed with fishmeal-based control diet or diet with CAP to replace 15%, 30%, 45%, 60% and 75% of fishmeal protein in control diet, respectively. From growth and feed utilization, the substitution level of CAP should no more than 30%, which was less than previous reported on juvenile turbot. The palatability of the feed was linearly reduced. Although CAP did not induce enteritis, it decreased the intestine villi height in middle and intestine villi density in distal intestine. Except for decreasing the size of hepatocytes by CAP at 45% or higher substitution levels, no other abnormality observed in liver histology. However, dietary CAP reduced the triglyceride and cholesterol levels in the plasma at 30% or higher levels and linearly down-regulated genes related to lipogenesis in liver. The genes related lipid transportation and oxidation were up-regulated by CAP at the substitution levels of 15–60%. The present work confirmed the safety of CAP in fish feed and suggested more work should be conducted before it widely used in aquafeed.

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Effects of fishmeal replacement by Clostridium autoethanogenum protein on the growth, digestibility, serum free amino acid and gene expression related to protein metabolism of obscure pufferfish (Takifugu obscurus)

Cited by 13 publications

References 44 publications

The flesh texture of teleost fish: Characteristics and interventional strategies

The flesh texture of teleost fish: Characteristics and interventional strategies

Growth, Cannibalism, and 5-TH Metabolism in Pufferfish (Takifugu obscurus ♀ × Takifugu rubripes): The Role of Graded Levels of Dietary Tryptophan

Effects of Clostridium autoethanogenum protein on growth performance, intestine and liver health of turbot Scophthalmus maximus during grow-out phase

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