Effects of flavonoid on calcium content in femoral tissue culture and parathyroid hormone-stimulated osteoclastogenesis in bone marrow culture in vitro

Yamaguchi, Masayoshi; Hamamoto, Reiko; Uchiyama, Satoshi; Ishiyama, Kaori

doi:10.1007/s11010-007-9458-x

Cited by 53 publications

(41 citation statements)

References 19 publications

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“…Recently, many researchers have reported that flavonoids showed good activity towards osteoporosis and inflammatory bone diseases. 13,14 In our work, the effects of isolated flavonoids compounds were carried out using a different cell line MC3T3-E1. Furthermore, the cell viability, ALP activity, collagen synthesis, and mineralization of MC3T3-E1 cell were studied systemically to check the effect of each sample.…”

Section: Resultsmentioning

confidence: 99%

Chemical Constituents from Acer mandshuricum and Their Effects on the Function of Osteoblastic MC3T3-E1 Cells

Ding¹,

Liang²,

Nguyen³

et al. 2010

Bulletin of the Korean Chemical Society

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A new compound, 4-methoxyl 5-hydroxymethyl benzoic 3-O-β-D-glucopyranoside (1), has been isolated from the leaves and stems of Acer mandshuricum, along with nine known compounds (2-10). Their structures were determined by a variety of spectroscopic analyses. The effect of compounds 1-10 on the function of osteoblastic MC3T3-E1 cells was examined by determining alkaline phosphatase (ALP) activity, collagen synthesis, and mineralization. Compound 1 significantly increased the function of osteoblastic MC3T3-E1 cells; 5.0 µM of 1 increased ALP activity, collagen synthesis, and mineralization of MC3T3-E1 cells to 114.7, 119.5, and 108.2% (P < 0.05) of the basal value, respectively. In addition, compounds 2-10 also potently increased the function of osteoblastic MC3T3-E1 cells.

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Section: Resultsmentioning

confidence: 99%

Chemical Constituents from Acer mandshuricum and Their Effects on the Function of Osteoblastic MC3T3-E1 Cells

Ding¹,

Liang²,

Nguyen³

et al. 2010

Bulletin of the Korean Chemical Society

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“…As recalled in the Introduction, flavonoids may interact with the biosynthesis of eumelanin, the most abundant pigment in blackbird feathers (McGraw, 2006). A key role in the link between flavonoids and moult may be played by a flavonoidmediated increase of calcium availability (Kanter et al, 2007;Yamaguchi et al, 2007). Melanin biosynthesis by melanocytes critically depends on limiting mineral elements, including calcium.…”

Section: Effects Of Dietary Flavonoids On Redox Status and Moultmentioning

confidence: 99%

Dietary flavonoids advance timing of moult but do not affect redox status of juvenile blackbirds (Turdus merula)

Cecere

Caprioli

Carnevali

et al. 2016

Journal of Experimental Biology

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Flavonoids are the most abundant plant polyphenols, widely occurring in fruits and berries, and show a strong antioxidant activity in vitro. Studies of avian species feeding on berries suggest that dietary flavonoids have health-promoting effects and may enhance the expression of melanin-based plumage traits. These effects are probably mediated by the antioxidant activity of flavonoids. However, the effect of dietary flavonoids on oxidative status has never been investigated in any bird species. We analysed the effects of dietary flavonoids on blood non-enzymatic antioxidants and protein oxidative damage of juvenile European blackbirds (Turdus merula). In addition, we analysed the effects of flavonoid-enriched diet on body condition and on the timing of moult from juvenile to adult plumage. Dietary flavonoids did not significantly affect redox status but significantly advanced the onset of moult, hastening plumage development. Moulting birds showed higher protein oxidative damage compared with those that had not yet started moulting. The probability of initiating moult after 40 days of dietary treatment was higher for birds with low circulating levels of oxidizing agents and high glutathione concentration. The metabolization of flavonoids could have altered their redox potential, resulting in no net effects on redox status. However, flavonoid consumption before and during moult may contribute to enhance plumage development. Moreover, our findings suggest that moulting feathers may result in redox imbalance. Given their effect on moult and growth of melanin-rich feathers, fruit flavonoids may have contributed to shape plant fruiting time in relation to fruit consumption preferences by birds.

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“…Studies have reported that quercetin antagonizes bone resorption in femoral organ cultures (13) and in osteoclast differentiation and/or activation assays in vitro (14,15) by a mechanism that involves suppression of NF-κB and AP-1 (16). Interestingly, a quercetin-like varient, quercetin-6-C-β-D-glucopyranoside isolated from Ulmus wallichiana planchon has been demonstrated to possess enhanced anti-osteoclastogenic activity and to mitigate ovariectomy-induced bone loss in rats (17).…”

Section: Introductionmentioning

confidence: 99%

“…Quercetin has been reported to inhibit the proliferation, differentiation, and mineralization of osteoblasts in vitro (24) and promote TNFα-induced inhibition (25) and apoptosis (25,26). Other studies however, report increased alkaline phosphatase (an early osteoblast differentiation marker) levels in human MG-63 osteoblast cells (27) and stimulatory effects on bone formation in rat organ cultures ex vivo (13).…”

Section: Introductionmentioning

confidence: 99%

Quercetin, a potent suppressor of NF-κB and Smad activation in osteoblasts

Weitzmann¹

2011

Int J Mol Med

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Abstract. Osteoclasts, the bone resorbing cells of the body, form when osteoclast precursors are exposed to the key osteoclastogenic cytokine receptor activator of NF-κB ligand (RANKL), a process requiring induction of NF-κB signaling. Quercetin is a ubiquitous plant-derived flavonoid with well documented antiinflammatory properties, in part, a consequence of its capacity to downmodulate the NF-κB signal transduction pathway. Consistent with this mechanism of action quercetin is reported to suppress osteoclastogenesis in vitro and prevent bone loss in ovariectomized mice in vivo. By contrast, the effect of quercetin on osteoblasts, the cells responsible for bone formation, is contradictory with conflicting reports of inhibition as well as stimulation. Given our previous reports that NF-κB antagonists promote osteoblast differentiation and activity, we compared the effects of quercetin on osteoclast and osteoblast differentiation and on NF-κB signal transduction in vitro. As expected, quercetin potently suppressed osteoclastogenesis and NF-κB activation induced by RANKL in osteoclast precursors. However, the same doses of quercetin had no effect on osteoblast mineralization, and failed to significantly alleviate the inhibitory effect of NF-κB-induced by TNFα, even though quercetin potently suppressed NF-κB activation in these cells. This apparent contradiction was explained by the fact that addition to its anti-NF-κB activity, quercetin also potently antagonized both TGFβ and BMP-2-induced Smad activation in osteoblast precursors. Taken together our data suggest that multiple competing actions of quercetin mediate both stimulatory and inhibitory actions on osteoblasts with the final physiological effect likely a function of the net balance between these stimulatory and inhibitory effects.

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Effects of flavonoid on calcium content in femoral tissue culture and parathyroid hormone-stimulated osteoclastogenesis in bone marrow culture in vitro

Cited by 53 publications

References 19 publications

Chemical Constituents from Acer mandshuricum and Their Effects on the Function of Osteoblastic MC3T3-E1 Cells

Chemical Constituents from Acer mandshuricum and Their Effects on the Function of Osteoblastic MC3T3-E1 Cells

Dietary flavonoids advance timing of moult but do not affect redox status of juvenile blackbirds (Turdus merula)

Quercetin, a potent suppressor of NF-κB and Smad activation in osteoblasts

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