Effects of Flaxseed Oil and Vitamin E Supplementation on Digestibility and Milk Fatty Composition and Antioxidant Capacity in Water Buffaloes

Agustinho, Bruna Calvo; Zeoula, Lúcia Maria; Santos, Nadine Woruby; Machado, Erica; Yoshimura, Emerson Henri; Ribas, Jessyca Caroline Rocha; Bragatto, Juliano; Stemposki, Mariana R; Santos, Vanessa Jorge dos; Faciola, A.P.

doi:10.3390/ani10081294

Cited by 6 publications

(3 citation statements)

References 41 publications

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“…VE supplement in diet could protect rats against deterioration of renal function by enhancing the GSH-Px activity in serum and decreasing the MDA content in kidney tissues (Haidara et al, 2009). VE supplementation also increased the antioxidant capacity and decreased oxidation products in milk (Agustinho et al, 2020). Both of the studies were consistent with our findings.…”

Section: Discussionsupporting

confidence: 91%

Vitamin E regulates bovine granulosa cell apoptosis via NRF2‐mediated defence mechanism by activating PI3K/AKT and ERK1/2 signalling pathways

Wang

Gao

et al. 2021

Reprod Domestic Animals

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High-yield dairy cows are usually subject to high-intensive cell metabolism and produce excessive reactive oxygen species (ROS). Once ROS is beyond the threshold of scavenging ability, it can induce oxidative stress, imperilling the reproductive performance of cows. The study was to investigate the effects of vitamin E (VE) on H 2 O 2 -induced proliferation and apoptosis of bovine granulosa cells and the underlying molecular mechanism. Granulosa cells were pretreated with VE for 24 hr and then treated with H 2 O 2 for 6 hr. The results showed that VE treatment decreased the intracellular ROS levels, increased the MDA content, and improved the antioxidant enzyme activity in a dose-dependent manner. Furthermore, VE treatment promoted the proliferation and inhibited apoptosis in granulosa cells by up-regulation of CCND1 and BCL2 levels and down-regulation of P21, BAX, and CASP3 levels. The cytoprotective effects of VE were attributed to the activation of the NRF2 signalling pathway. Knockdown of the NRF2 impaired the cytoprotective effects of VE on granulosa cells. Besides, the PI3K/AKT and ERK1/2, but not the p38 signalling pathway is involved in the regulation of VE-mediated cell proliferation and apoptosis. The PI3K/AKT inhibitor LY294002 and ERK1/2 inhibitor SCH772984 inhibited the VE-induced granulosa cell proliferation and promoted apoptosis, whereas the p38 inhibitor SB203580 had the opposite effects. These results were confirmed by proliferation and apoptosis-related gene expression at mRNA and protein levels.The results also showed that the PI3K/AKT inhibitor LY294002 and ERK1/2 inhibitor SCH772984 inhibited VE-induced NRF2, GCLC, GCLM, and HO-1 expression, whereas the p38 inhibitor SB203580 not. Overall, the results demonstrated that VE-regulated granulosa cell proliferation and apoptosis via NRF2-mediated defence system by activating the PI3K/AKT and ERK1/2 signalling pathway.

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Section: Discussionsupporting

confidence: 91%

Vitamin E regulates bovine granulosa cell apoptosis via NRF2‐mediated defence mechanism by activating PI3K/AKT and ERK1/2 signalling pathways

Wang

Gao

et al. 2021

Reprod Domestic Animals

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“…The intake of vitamins and antioxidants within the diet is transferred to milk. Supplementing vitamin E in the diet can increase the antioxidant capacity of milk and decrease the products of oxidation [ 10 ]. In buffalo milk, there are not carotenoids but a high content of vitamin A.…”

Section: Introductionmentioning

confidence: 99%

Chemical Characteristics and Oxidative Stability of Buffalo Mozzarella Cheese Produced with Fresh and Frozen Curd

Rinaldi¹,

Palocci²,

Giovanni³

et al. 2021

Molecules

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Milk and dairy products can have variable contents of antioxidant compounds that contribute to counteract the oxidation of lipids and proteins during processing and storage. The content of active antioxidant compounds is closely linked to their protection by oxidation. Freezing is one of the factors that can reduce antioxidant activity. Freezing of milk or curd is frequently used in case of the seasonality of milk production and/or seasonal increased demand for some products. In this paper, the effect of using frozen curd on the oxidative stability of buffalo Mozzarella cheese was evaluated. Samples of buffalo Mozzarella with different frozen curd content (0%, 5%, 20%, and 50%) were produced and analyzed at one and nine days. Mozzarella cheese with higher frozen curd content had a significant increase in redox potential parallel to the decrease in antioxidant activity, showing less protection from oxidation. Lipid and protein oxidation, expressed respectively by malondialdehyde and carbonyl content, increased significantly with increasing frozen curd. At nine days, carbonyls significantly increased while malondialdehyde content did not vary, showing that during storage, fat was more protected from oxidation than protein. The average carbonyl levels were comparable to those of some cooked cheeses, and the malondialdehyde levels were even lower. The results of this study stimulate the investigation of new strategies to decrease the oxidative damage in cheeses produced in the presence of factors decreasing oxidative stability.

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“…To achieve higher levels of milk production, the diet that the buffaloes receive is an important factor. In this sense, it is important to understand the efficient use of protein and energy and their utilisation for ruminal microorganism growth and microbial protein production (Paul et al ., 2003; Santillo et al ., 2016; Reddy et al ., 2019) Hence it is necessary to evaluate the pattern of diets offered to the buffaloes (Paul et al ., 2003; Santillo et al ., 2016; Agustinho et al ., 2020). Regarding nitrogen (N) compound metabolism and the interaction of protein and non-protein nitrogen (NPN) for the N-balance for lactating ruminants, there are two essential metabolite parameters.…”

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confidence: 99%

Effect of replacing soybean meal by a blend of ground corn and urea-ammonium sulphate on milk production and composition, digestibility and N balance of dairy Murrah buffaloes

Melo

Bezerra

Santos

et al. 2022

Journal of Dairy Research

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This study evaluated the effect of replacing soybean meal by a blend of ground corn and urea-ammonium sulphate (GCU-S) in the diet of lactating buffaloes on milk production and composition, digestibility, N balance and blood metabolites. Twelve multiparous dairy Murrah buffaloes (Bubalus bubalis), at 100 ± 4 d in milk and yielding 10 ± 2.5 kg/d, were randomly distributed in a triple 4 × 4 Latin square, with four different inclusions of GCU-S at U-S levels: 0 (control), 8.0, 16.4 and 24.1 g/kg dry matter (DM) total. Replacing soybean meal with GC-US had a significant depressing effect on absolute DM intake, which was still numerically evident but no longer significant when expressed on a body weight-related basis. Intakes of crude protein (CP) and N as well as N-urinary excretion were also significantly depressed. Digestibility of dry matter was improved and, as a proportion of DMI, intake of total digestible nutrients increased significantly. Perhaps as a result, feed efficiency (kg DMI required per kg 6% fat-corrected milk) was significantly improved. Efficiency of protein use for milk production was significantly improved, but not when expressed as milk protein output. N transfer into milk, as a proportion of total N intake, also increased significantly. However, GC-US inclusion had no significant effect on milk production or milk composition, nor did it affect serum metabolites, digestibilities (apart from dry matter) or N measures of balance apart from those mentioned above. Inclusion of this blend of ground corn with urea and ammonium suplate can be recommended for dairy buffalo because, although it decreases DMI and N-excretion, it improves DM digestibility and feeding efficiency whilst maintaining milk production and composition.

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Effects of Flaxseed Oil and Vitamin E Supplementation on Digestibility and Milk Fatty Composition and Antioxidant Capacity in Water Buffaloes

Cited by 6 publications

References 41 publications

Vitamin E regulates bovine granulosa cell apoptosis via NRF2‐mediated defence mechanism by activating PI3K/AKT and ERK1/2 signalling pathways

Vitamin E regulates bovine granulosa cell apoptosis via NRF2‐mediated defence mechanism by activating PI3K/AKT and ERK1/2 signalling pathways

Chemical Characteristics and Oxidative Stability of Buffalo Mozzarella Cheese Produced with Fresh and Frozen Curd

Effect of replacing soybean meal by a blend of ground corn and urea-ammonium sulphate on milk production and composition, digestibility and N balance of dairy Murrah buffaloes

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