Betaine, a donor of labile methyl groups, can spare choline and methionine but cannot replace these compounds in poultry diets. Betaine is synthesized from choline by choline oxidase and it can donate methyl groups to homocysteine to form methionine. Physiologically, betaine is one of several compounds used by cells to regulate osmotic pressure. Among the potential benefits of its inclusion in poultry feeds are sparing choline, carcass fat reduction and aiding cell osmoregulation. Some feed ingredients are natural sources of betaine per se. This review considers the metabolism, functions and applications of betaine in poultry. Betaine, metabolic by-product or vital methylating agent? Life BELL, A. (1995) What's the word on betaine? Pork95, February, pp. 26-27 BURG, M.B. (1994) Molecular basis for osmoregulation of organic osmolytes in renal medullary cells. The osmoprotective properties of urine for bacteria: The protective effect of betaine and human urine against low pH and high concentrations of electrolytes, sugars, and urea. Journal of Infectious Diseases 152: 1308-1316 CHAMBERS, S.T. and KUNIN, C.M. (1987) Osmoprotective activity for Escherichia coli in mammalian renal inner medulla and urine. The efflux of betaine from rat-liver mitochondria, a possible regulating step in choline oxidation. Biochimica et Biophysica Acta 291: 557-563 DE RIDDER, J.J.M. and VAN DAN, K. (1975) Control of choline oxidation by rat-liver mitochondria. Biochimica et Biophysica Acta 408: 112-122 261: 5872-5877 123 Sciences 32: 771-774 Journal of Experimental Zoology 268: 171-175 DU VIGNEAUD, V., CHANDLER, J.P., MOYER, A.W. and KEPPEL, D.M. (1939) The effect of choline on the ability of homocystine to replace methionine in the diet. loicrnal of Biological Chemistry 131: 57-76