Effects of four cryoprotectants in combination with two vehicle solutions on cultured vascular endothelial cells

Pollock, Graeme A.; Hamlyn, L.; Maguire, S.H.; Stewart-Richardson, P.A.; Hardie, Ian R.

doi:10.1016/0011-2240(91)90049-t

Cited by 15 publications

(3 citation statements)

References 20 publications

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“…For example, the larval survival is 95% when, after 1·h exposure, the tissue concentration of methanol reaches 1.13·mol·l -1 , and the survival is about 93% after exposure to 1.5·mol·l -1 EG for 3.5·h, at which time the EG concentration in the larvae rises to the same concentration of 1.1·mol·l -1 . EG and methanol have been reported to be less toxic to a variety of cells and tissues than other commonly used CPAs such as DMSO and glycerol (Ali and Shelton, 1993;Pollock et al, 1991;Zhang and Rawson, 1996a). Our study shows that high percentages (>90%) of Anopheles larvae survive the exposure in EG and methanol for a sufficient time period that allows them to permeate up to reasonably high concentrations without excessive injury.…”

Section: Discussionmentioning

confidence: 55%

Permeation and toxicity of ethylene glycol and methanol in larvae of Anopheles gambiae

Liu

Pan

Mazur

2003

Journal of Experimental Biology

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Mosquitoes are, by far, the deadliest creatures on the planet. They transmit malaria, yellow fever, dengue fever, encephalitis and other diseases to more than 700 million people each year. Among them, malaria, transmitted by Anopheles mosquitoes, is the worst and it infects nearly half a billion people, resulting in several million deaths worldwide each year (WHO, 1996). Malaria control strategies have been proposed in which genes for resistance to malaria will be introduced into vector populations in order to convert normal vector populations to mosquitoes that are unable to transmit malaria (Collins et al., 1986;Ito et al., 2002;Zheng et al., 1997). The recently complete genome sequence of both Anopheles gambiae (Holt et al., 2002) and the malaria parasite Plasmodium falciparum (Gardner et al., 2002) ought to accelerate the development of engineered mosquitoes refractory to Plasmodium and of methods for driving this genotype into the wild population. Such genetic studies will require the creation, maintenance and characterization of many genetic lines; however, such maintenance with present breeding techniques, in addition to being costly and time consuming, has problems of crosscontamination among strains, loss of stains through handling errors, and genetic changes caused by laboratory domestication or genetic drift. The ability to preserve Anopheles eggs or larvae cryobiologically would eliminate or substantially ameliorate these problems.Cryopreservation of any biological material requires that cryoprotective agents (CPAs) are present in the cells either to prevent so-called 'solution-effect' injury (Mazur, 1970) during classical slow-freezing procedures or to prevent intracellular ice formation during vitrification procedures that involve cooling at high rates. The former approach is used in the cryopreservation of most mammalian cells; the latter, vitrification, was used by Steponkus et al. (1990) and Mazur et al. (1992a) in the successful cryopreservation of Drosophila embryos. Both approaches demand that the cells in question be permeable to both water and CPAs. Unfortunately, native Anopheles eggs show poor permeability to water and are essentially impermeable to ethylene glycol (EG; Valencia et al., 1996a), the CPA used in the cryopreservation of Drosophila (Mazur et al., 1992a;Steponkus et al., 1990) and house flies (Wang et al., 2000). Native eggs of Drosophila and house flies are also impermeable to both water and CPA; however, procedures were developed to successfully permeabilize them and thereby permit cryopreservation. The permeability barrier in the eggs of these two species appears to be a wax layer lying on the surface of the endochorion In this study, we applied proton NMR to measure the permeation of two cryoprotective agents (CPAs), ethylene glycol (EG) and methanol, into 1st instar Anopheles larvae. Calibration with standard solutions of EG or methanol (0-10·mol·l -1 ) confirmed the reliability of the NMR measurements for determining the concentration of these solutes. To assess permeati...

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Section: Discussionmentioning

confidence: 55%

Permeation and toxicity of ethylene glycol and methanol in larvae of Anopheles gambiae

Liu

Pan

Mazur

2003

Journal of Experimental Biology

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“…High Me 2 SO concentration (3M) is cytotoxic to porcine endothelial cells [30], but there is conflicting evidence regarding the toxicity of lower Me 2 SO concentrations on fibroblast and endothelial cells that could be used to repopulate decellularized vascular tissue. Although the literature describes conflicting effects of Me 2 SO on the function of various cell types, it is clear that a very low concentration of Me 2 SO must be achieved to avoid potential confounding effects when using cryopreserved heart valves for novel tissueengineered constructs.…”

Section: Discussionmentioning

confidence: 99%

NMR Assessment of Me2SO in Decellularized Cryopreserved Aortic Valve Conduits

Lehr

Hermary

McKay

et al. 2007

Journal of Surgical Research

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“…Increasing glycerol in the alginate solution up to 30% resulted in significantly higher survival rates. However, when a higher concentration of glycerol was used (up to 70%), the survival percentage decreased, probably due to osmotic disruption of the cells at high glycerol concentration (26,27) within the dried preparation.…”

Section: Survival Of P Agglomerans Strain Ic1270 Duringmentioning

confidence: 99%

Preservation of Chitinolytic Pantoae agglomerans in a Viable Form by Cellular Dried Alginate‐Based Carriers

Zohar‐Perez¹,

Ritte²,

Chernin³

et al. 2002

Biotechnology Progress

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Improved viability of Gram-negative bacteria during freeze-dehydration, storage, and soil inoculation is of crucial importance to their efficient application. The chitinolytic Pantoae (Enterobacter) agglomerans strain IC1270, a potential biocontrol agent of soil-borne plant-pathogenic fungi, was used as a model organism to study the efficacy of freeze-dried alginate-based beads (macrocapsules) as possible carriers for immobilized Gram-negative bacterial cells. These macrocapsules were produced by freeze-dehydration of alginate gel spherical beads, in which different amounts of bacteria, glycerol, and colloidal chitin were entrapped. Subsequent drying produced different unexpected structures, pore-size distributions, and changes in the outer and inner appearance of the resultant dried cellular solid. With increasing glycerol content, the proportion of larger pores increased. These structures can be related to changes in the slow-release properties of the dried beads. The amount of glycerol in the beads differed from that in the alginate solution as a result of leakage during the beads' preparation and dehydration. Entrapping 10(9) cells per bead produced from alginate solution containing 30% glycerol and 1% chitin resulted in improved (in comparison to other studies) survival prospects (95%) during freeze-drying. Moreover, immobilization of the bacterium sharply improved its survival in nonsterile irrigated and dry soils compared to bacteria in a water suspension. The results suggest that optimized conservation of Gram-negative bacteria in dry glycerol-containing alginate-based cellular solids is not only possible but applicable for a variety of uses.

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Effects of four cryoprotectants in combination with two vehicle solutions on cultured vascular endothelial cells

Cited by 15 publications

References 20 publications

Permeation and toxicity of ethylene glycol and methanol in larvae of Anopheles gambiae

Permeation and toxicity of ethylene glycol and methanol in larvae of Anopheles gambiae

NMR Assessment of Me2SO in Decellularized Cryopreserved Aortic Valve Conduits

Preservation of Chitinolytic Pantoae agglomerans in a Viable Form by Cellular Dried Alginate‐Based Carriers

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