Effects of fundamental nutrient stresses on the lipid accumulation profiles in two diatom species Thalassiosira weissflogii and Chaetoceros muelleri

Lin, Qun; Zhuo, Wen-Hao; Wang, Xinwei; Chen, Changping; Gao, Yahui; Liang, Junrong

doi:10.1007/s00449-018-1950-z

Cited by 33 publications

(25 citation statements)

References 40 publications

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“…The highest total lipid content was observed in culture with the lowest concentration of P (7 µM), and it was observed that the total lipid content decreased as the P concentration increased. It has been widely documented in several species of microalgae that under phosphate deficiency, accumulation of neutral lipids is a common response and it has been observed that phospholipid levels in cells are reduced and being repla- Lin et al (2018) are higher than those reported in this study, it is worth to mention that the laboratory's conditions vary among them, as well as the experimental design, the culture media, and the strains; these factors could influence in the final results. On the other hand, although the protein content was higher in culture with the lowest concentration of P (7 µM), no significant differences were found among treatments, but we did find differences between the treatments of P starvation (7 and 18 µM).…”

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confidence: 59%

Effect of different phosphorus concentrations on growth and biochemical composition of Chaetoceros muelleri

Lovio-Fragoso¹,

Hayano-Kanashiro²,

López‐Elías³

2019

LAJAR

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Chaetoceros muelleri is one of the most widely used microalgae species in aquaculture in northwestern Mexico as food for fish and crustaceans. Its importance is due to its fast growth, nutritional quality and lipid accumulation under nutrient-limiting conditions. However, the biochemical and molecular mechanisms of uptake of phosphorus (P) limiting conditions for this species is still unknown. This study aimed to analyze the growth and biochemical composition of C muelleri in response to different phosphorus concentrations. Four treatments under different phosphorus conditions were used: control (72 µM P), excess (144 µM P) and two treatments with phosphorus limitation, limited A (18 µM P) and limited B (7 µM P). The highest cell concentration was observed in control and excess of P (>3.4×10 6 cells mL -1 ). The highest dry mass concentration and chlorophyll-a content were found in control medium (72 µM P) whereas the highest total lipid (4.42%) was found in the lowest P-concentration medium (7 µM P). No significant differences were found in the total protein content, but we did find differences between the treatments with phosphorus limitation (7 and 18 µM P).

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confidence: 59%

Effect of different phosphorus concentrations on growth and biochemical composition of Chaetoceros muelleri

Lovio-Fragoso¹,

Hayano-Kanashiro²,

López‐Elías³

2019

LAJAR

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“…Lipid bodies (LBs) are produced under different stress conditions like nutrient depletion, high light, metal contamination etc. (d'Ippolito et al, 2015;Lin et al, 2018). In the present study, we made an effort to study the number of LBs and their size in most commonly occurring diatoms species during initial and final growth phases using microscopic analysis (Table 2).…”

Section: Lipid Contentmentioning

confidence: 99%

Biodiesel production through algal cultivation in urban wastewater using algal floway

Marella

Datta

Patil

et al. 2019

Bioresource Technology

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A B S T R A C TThe aim of this work was to study algal floway (AFW) to treat urban wastewater and to evaluate biomass productivity, lipid contents and biodiesel production. The results indicated the seasonal average algae productivity of 34.83 g dry weight m 2 d −1 with a nutrient removal rate of 2.52 g m 2 d −1 N and 1.25 g m 2 d −1 P while the lipid content ranged between 14 and 22% of dry cell weight with the highest lipid productivity of 9.29 g m −2 d −1 during summer. Biodiesel quality was superior during summer with high centane number and cold filter plugging point values. High eicosapentaenoic acid content was found during winter growth cycles. AFW algae community was dominated by pennate diatoms during all growing seasons. This study is one of its kinds in Indian wastewaters and it provides fundamental information for further optimization and use of AFW to treat domestic wastewater and to produce algae biofuel feedstock.quantities of untreated domestic wastewater in urban and semi-urban areas, using this water as a source for nutrients for algae feedstock production is an attractive option to offset the biodiesel production costs (Craggs et al., 2011).Algae culture technologies like raceway ponds and photobioreactors are in use for decades but they are expensive and energy intensive due to which net energy recovered from algae oil is high (Chisti, 2013). Method of cultivation plays a key role in how the biomass can be https://doi.

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“…Increase in lipid accumulation after nitrogen starvation has been observed in some diatom species, such as Phaeodactylum tricornutum, Chaetoceros muelleri, and Thalassiosira weissflogii [16,17]. Other nutrient limitation stresses involved in an increased lipid accumulation are phosphorous and silicon deprivation [17,18]. In Chaetoceros gracilis, a combined mild Biology 2020, 9, 5 2 of 17 deprivation of both silicon and sodium chloride produced a lipid accumulation reaching up to 73% of the total cell dry weight [10].…”

Section: Introductionmentioning

confidence: 99%

“…Several studies show that lipids can accumulate in diatoms after mild or acute nutrient deprivation, generally at the expense of a decrease in biomass [14,15]. Increase in lipid accumulation after nitrogen starvation has been observed in some diatom species, such as Phaeodactylum tricornutum, Chaetoceros muelleri, and Thalassiosira weissflogii [16,17]. Other nutrient limitation stresses involved in an increased lipid accumulation are phosphorous and silicon deprivation [17,18].…”

Section: Introductionmentioning

confidence: 99%

Storage Compound Accumulation in Diatoms as Response to Elevated CO2 Concentration

Jensen

Yangüez

Carrière

et al. 2019

Biology

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Accumulation of reserve compounds (i.e., lipids and chrysolaminarin) in diatoms depends on the environmental conditions, and is often triggered by stress conditions, such as nutrient limitation. Manipulation of CO2 supply can also be used to improve both lipids and carbohydrates accumulation. Given the high diversity among diatoms, we studied the two marine model diatoms—Thalassiosira pseudonana and Phaeodactylum tricornutum, a freshwater diatom, Asterionella formosa, and Navicula pelliculosa—found in fresh- and sea-water environments. We measured the accumulation of reserve compounds and the activity of enzymes involved in carbon metabolism in these diatoms grown at high and atmospheric CO2. We observed that biomass and lipid accumulation in cells grown at high CO2 differ among the diatoms. Lipid accumulation increased only in P. tricornutum and N. pelliculosa grown in seawater in response to elevated CO2. Moreover, accumulation of lipids was also accompanied by an increased activity of the enzymes tested. However, lipid accumulation and enzyme activity decreased in N. pelliculosa cultured in fresh water. Chrysolaminarin accumulation was also affected by CO2 concentration; however, there was no clear relation with lipids accumulation. Our results are relevant to understand better the ecological role of the environment in the diatom adaptation to CO2 and the mechanisms underpinning the production of storage compounds considering diatom diversity.

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Effects of fundamental nutrient stresses on the lipid accumulation profiles in two diatom species Thalassiosira weissflogii and Chaetoceros muelleri

Cited by 33 publications

References 40 publications

Effect of different phosphorus concentrations on growth and biochemical composition of Chaetoceros muelleri

Effect of different phosphorus concentrations on growth and biochemical composition of Chaetoceros muelleri

Biodiesel production through algal cultivation in urban wastewater using algal floway

Storage Compound Accumulation in Diatoms as Response to Elevated CO2 Concentration

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