Effects of gamma ray dose rate and sucrose treatment on mutation induction in chrysanthemum

Kim, Ye-Sol; Sung, Sang Yeop; Jo, Jeong Deuk; Lee, Hyo-Jeong; Kim, Sang Hoon

doi:10.17660/ejhs.2016/81.4.4

Cited by 17 publications

(11 citation statements)

References 22 publications

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“…Semi-lethal state was recorded at 35 Gy. Similar results were obtained in other chrysanthemum genotypes after treatment with gamma rays (Kim et al, 2016). However, the growth of 'Donglinruixue' plants was significantly inhibited with increasing radiation dose.…”

Section: Discussionsupporting

confidence: 85%

Morphological, cytological and molecular variations induced by gamma rays in Chrysanthemum morifolium ‘Donglinruixue’

Lan

Gao

2020

Folia Horticulturae

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The current study investigated the effects of gamma radiation on the death rate, morphological traits and meiotic abnormalities in ground-grown chrysanthemum ‘Donglinruixue’, and inter-simple sequence repeat (ISSR) markers were used to identify the DNA polymorphism among mutants. The results showed that the death rate significantly increased with increase in radiation dose. Semi-lethal (LD50) dose was approximately 35 Gy. Compared with unirradiated control, plant growth was significantly inhibited. After irradiation, a series of morphological variations and cytological aberrations occurred in radiated plants. The peak in variation frequency appeared at 35 Gy. In total, ISSR analysis produced 72 scorable bands, of which 64 (88.89%) were polymorphic. The current study demonstrated that gamma irradiation generates a sufficient number of induced mutations and that ISSR analysis offered a useful molecular marker analysis for the identification of mutants.

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Section: Discussionsupporting

confidence: 85%

Morphological, cytological and molecular variations induced by gamma rays in Chrysanthemum morifolium ‘Donglinruixue’

Lan

Gao

2020

Folia Horticulturae

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“…It can induce morphological changes in plant tissues and various biochemical changes at the cellular level to form new mutants that have new productivity potential, produce higher metabolite content and develop varieties with better economic and agronomic characteristics (Wi et al 2007;Rahimi and Bahrani 2011). In Chrysanthemum flowers, gamma irradiation causes changes in the shape and color of flowers (Kim et al 2016). Changes in the morphological character of plants due to gamma irradiation also occur in Polianthes tuberosa, Delphinium malabaricum, and Rosa hybrida.…”

Section: Introductionmentioning

confidence: 99%

The diversity of morphological characteristics and chemical content of Celosia cristata plantlets due to gamma ray irradiation

Muhallilin¹,

Aisyah

Sukma³

2019

Biodiversitas

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Abstract. Muhallilin I, Aisyah SI, Dewi Sukma D. 2019. The diversity of morphological characteristics and chemical content of Celosia cristata plantlets due to gamma ray irradiation. Biodiversitas 20: 862-866. Celosia cristata L. or better known as Jengger Ayam is an ornamental plant that has a unique flower shape with attractive colors. Besides, this plant also contains antioxidants and chemical compounds that are used for traditional medicine to treat high blood pressure, and diabetes or as eye ailments. Increased diversity of C. cristata through hybridization techniques is constrained by flowers forming small grains that makes it difficult for castration and isolation of reproductive organs. This study aimed to increase C. cristata diversity of morphology and chemical content using mutation techniques induced by gamma irradiation. Plantlets were irradiated at 0, 25, 50 and 75 Gy doses. Scoring was conducted for 5 weeks until MV6 generation. Planlets were acclimatized for 3 weeks. The surviving plants were moved onto experimental land until seeded and tested for phytochemicals. Gamma irradiation with 25, 50 and 75 Gy doses produced 4 clones that have morphological changes in color and shape of the stem, leaves, and flowers. Changes in chemical content were observed with the appearance of triterpenoid compounds in 1 clone irradiated with 25 Gy.

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“…In contrast, the mutation frequency is dependent on the total dose, not the dose rate, in chrysanthemum [23]. Recently, Kim et al [24] proposed that a specific irradiation duration could induce a higher mutation frequency and broader spectrum of mutants in chrysanthemum. In the present study, we used the RD 50 based on the multiplication and relative regeneration rates as a guide to construct mutant populations from two Cymbidium cultivars (Figure 1).…”

Section: Optimal γ-Irradiation Condition For Mutation Inductionmentioning

confidence: 99%

“…Irradiation dose has been mainly used for determination of the optimal irradiation condition in diverse plant species, including orchids [5,8,[13][14][15][16][17][18]. However, the optimal doses suggested by previous researchers are diverse: e.g., LD 10 in rice seeds [18]; LD and RD in crop seeds [19]; LD [20][21][22][23][24][25][26][27][28][29][30] in in vitro tissues [20]; and RD 50 in Cymbidium protocorm-like bodies (PLBs) [9]. Furthermore, irradiation duration and dose rate, a complex concept of dose and duration, are also important factors for induction of mutations.…”

Section: Optimal γ-Irradiation Condition For Mutation Inductionmentioning

confidence: 99%

“…Kim et al [24] reported that at an identical total γ-ray dose of 30 Gy, an irradiation duration of 4 h among durations of 1, 4, 8, 16, and 24 h induced the highest frequency of flower-color mutations in chrysanthemum. Kodym et al [19] suggested that radiation-induced DNA damage is restored by a repair mechanism mainly in the S phase of the DNA replication cycle and that a long duration of irradiation reduces the effects of the radiation dose.…”

Section: Effects Of Short-term Irradiation and Re-irradiation On Mutamentioning

confidence: 99%

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Frequency, Spectrum, and Stability of Leaf Mutants Induced by Diverse γ-Ray Treatments in Two Cymbidium Hybrids

Kim

Ahn

et al. 2020

Plants

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Ionizing radiation combined with in vitro tissue culture has been used for development of new cultivars in diverse crops. The effects of ionizing radiation on mutation induction have been analyzed on several orchid species, including Cymbidium. Limited information is available on the comparison of mutation frequency and spectrum based on phenotypes in Cymbidium species. In addition, the stability of induced chimera mutants in Cymbidium is unknown. In this study, we analyzed the radiation sensitivity, mutation frequency, and spectrum of mutants induced by diverse γ-ray treatments, and analyzed the stability of induced chimera mutants in the Cymbidium hybrid cultivars RB003 and RB012. The optimal γ-irradiation conditions of each cultivar differed as follows: RB003, mutation frequency of 4.06% (under 35 Gy/4 h); RB012, 1.51% (20 Gy/1 h). Re-irradiation of γ-rays broadened the mutation spectrum observed in RB012. The stability of leaf-color chimera mutants was higher than that of leaf-shape chimeras, and stability was dependent on the chimera type and location of a mutation in the cell layers of the shoot apical meristem. These results indicated that short-term γ-irradiation was more effective to induce mutations in Cymbidium. Information on the stability of chimera mutants will be useful for mutation breeding of diverse ornamental plants.Plants 2020, 9, 546 2 of 11 breeding [6]. However, development of a new Cymbidium cultivar by means of cross-breeding is time-consuming because of the long vegetative growth stage.Mutation breeding techniques using physical mutagens (e.g., γ-rays, X-rays, and ion particles) or chemical mutagens (e.g., ethyl methanesulfonate, N-nitroso-N-methylurea, and colchicine) have been widely used to develop mutant cultivars in diverse plant species, including food and ornamental crops. In 226 plant species, 3308 mutants, including two Cymbidium mutants, that were predominantly (77.5%) developed using physical mutagens have been registered in the Mutant Variety Database of the joint Food and Agriculture Organization of the United Nations/International Atomic Energy Agency [7]. Among the diverse ornamental plants registered on the database, the most numerous mutants are those of chrysanthemum (Chrysanthemum spp., 274 mutants) followed by rose (Rosa spp., 67 mutants), dahlia (Dahlia spp., 36 mutants), alstroemeria (Alstroemeria spp., 35 mutants), streptocarpus (Streptocarpus spp., 30 mutants), and carnation (Dianthus caryophyllus, 28 mutants) [7]. The combination of treatment with a physical mutagen and in vitro tissue culture has been used to shorten the breeding period of orchids [8,9]. Thus, integration of γ-irradiation and in vitro tissue culture may be an efficient procedure for development of mutant cultivars in Cymbidium.The treatment of seeds, buds (tip/node cuttings), callus, and rhizomes with a mutagen induces chimeras in M 1 plants because mutations are induced in individual cells and regenerated shoots are recovered from pre-existing multicellular meristems [10]. In seed-propagated pl...

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Effects of gamma ray dose rate and sucrose treatment on mutation induction in chrysanthemum

Cited by 17 publications

References 22 publications

Morphological, cytological and molecular variations induced by gamma rays in Chrysanthemum morifolium ‘Donglinruixue’

Morphological, cytological and molecular variations induced by gamma rays in Chrysanthemum morifolium ‘Donglinruixue’

The diversity of morphological characteristics and chemical content of Celosia cristata plantlets due to gamma ray irradiation

Frequency, Spectrum, and Stability of Leaf Mutants Induced by Diverse γ-Ray Treatments in Two Cymbidium Hybrids

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