Effects of gangliosides on the differentiation of human mesenchymal stem cells into osteoblasts by modulating epidermal growth factor receptors

Kim, Sunmi; Jung, Ji-Ung; Ryu, Jae‐Sung; Jin, Jung-Woo; Yang, Hyo-Jung; Ko, Kisung; You, Hyung‐Keun; Jung, Kyu-Yong; Choo, Young‐Kug

doi:10.1016/j.bbrc.2008.04.162

Cited by 44 publications

(76 citation statements)

References 17 publications

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“…The expression of these gangliosides was also found in human dental pulp-derived stem cells [75] and adipose-derived stem cells [83]. The expression patterns of these a-series gangliosides in mesenchymal stem cells were found to change during their neuronal or osteoblast differentiation [51,75,82,83].…”

Section: Glycolipids In Multipotent Stem Cellmentioning

confidence: 87%

“…In human mesenchymal stem cells prepared from periodontal bone marrow, only GM1 was identified by thin-layer chromatography and thin-layer chromatography-immunostaining [51]. In human mesenchymal stem cells from dental papilla, GM3, GM2, and GD1a, but not GM1, were biochemically detected [82]. The expression of these gangliosides was also found in human dental pulp-derived stem cells [75] and adipose-derived stem cells [83].…”

Section: Glycolipids In Multipotent Stem Cellmentioning

confidence: 96%

“…In mesenchymal stem cells isolated from human periodontal ligament, SSEA-4 and SSEA-1 have been found by flow cytometric and immunocytochemical analyses [88]. Nevertheless, GD2 and SSEA-4 have not been biochemically detected in ganglioside fractions extracted from mesenchymal stem cells in bone marrow, dental papilla, dental pulp, and adipose [51,75,82,83]. These gangliosides may be tissue type-specifically expressed or quite minor components in mesenchymal stem cells.…”

Section: Glycolipids In Multipotent Stem Cellmentioning

confidence: 99%

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Stem Cell Glycolipids

Yanagisawa

2010

Neurochem Res

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Glycolipids are compounds containing one or more monosaccharide residues bound by a glycosidic linkage to a hydrophobic moiety. Because of their expression patterns and the intracellular localization patterns, glycolipids, including stage-specific embryonic antigens (SSEA-3, SSEA-4, and possibly SSEA-1) and gangliosides (e.g., GD3, GD2, and A2B5 antigens), have been used as marker molecules of stem cells. In this review, I will introduce glycolipids expressed in pluripotent stem cells (embryonic stem cells, induced pluripotent stem cells, very small embryonic-like stem cells, amniotic stem cells, and multilineage-differentiating stress enduring cells), multipotent stem cells (neural stem cells, mesenchymal stem cells, fetal liver multipotent progenitor cells, and hematopoietic stem cells), and cancer stem cells (brain cancer stem cells and breast cancer stem cells), and discuss their availability as biomarkers for identifying and isolating stem cells.

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Section: Glycolipids In Multipotent Stem Cellmentioning

confidence: 87%

Section: Glycolipids In Multipotent Stem Cellmentioning

confidence: 96%

Section: Glycolipids In Multipotent Stem Cellmentioning

confidence: 99%

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Stem Cell Glycolipids

Yanagisawa

2010

Neurochem Res

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“…The expression and functionality of EGFR has been clearly demonstrated in bone marrow mesenchymal stem cells (BMMSCs) [6,28], and osteoblasts themselves differentiate from mesenchymal stem cells [29]. In addition, EGFR ligands, such as EGF and amphiregulin, stimulate the proliferation of BMMSCs and osteoblasts [30][31][32], suggesting that EGFR ligands are mitogens for BMMSCs and osteoblasts.…”

Section: Discussionmentioning

confidence: 99%

Erlotinib prevents experimental metastases of human small cell lung cancer cells with no epidermal growth factor receptor expression

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Hashimoto

et al. 2011

Clin Exp Metastasis

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Epidermal growth factor receptor-tyrosine kinase inhibitors (EGFR-TKIs) show dramatic antitumor activity in a subset of patients with non-small cell lung cancer who have an active mutation in the epidermal growth factor receptor (EGFR) gene. On the other hand, some lung cancer patients with wild type EGFR also respond to EGFR-TKIs, suggesting that EGFR-TKIs have an effect on host cells as well as tumor cells. However, the effect of EGFR-TKIs on host microenvironments is largely unknown. A multiple organ metastasis model was previously established in natural killer cell-depleted severe combined immunodeficient mice using human lung cancer cells. This model was used to investigate the therapeutic efficacy of erlotinib, an EGFR-TKI, on multiple organ metastases induced by human small cell lung cancer cells (SBC-5 cells) that did not express EGFR. Although erlotinib did not have any effect on the proliferation of SBC-5 cells in vitro, it significantly suppressed bone and lung metastases in vivo, but not liver metastases. An immunohistochemical analysis revealed that, erlotinib significantly suppressed the number of osteoclasts in bone metastases, whereas no difference was seen in microvessel density. Moreover, erlotinib inhibited EGF-induced receptor activator of nuclear factor kappa-B expression in an osteoblastic cell line (MC3T3-E1 cells). These results strongly suggested that erlotinib prevented bone metastases by affecting host microenvironments irrespective of its direct effect on tumor cells.

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“…Osteogenic differentiation was initiated using previously published methods (Ikeda et al, 2006;Kim et al, 2008). The cells were cultured in maintenance medium supplemented with 10 mM β-glycerophosphate (Sigma), 25 µg/mL of L-ascorbic acid (Sigma), and 100 nM dexamethasones (Sigma) for 5 days.…”

Section: Induction Of Osteogenic Differentiation Of Hadscs and Hdpscsmentioning

confidence: 99%

Comparison of ganglioside expression between human adipose- and dental pulp-derived stem cell differentiation into osteoblasts

et al. 2010

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Human adipose-derived stem cells (hADSCs) and dental pulp-derived stem cells (hDPSCs) have been considered alternative sources of adult stem cells because of their potential to trans-differentiate into multiple cell lineages. This study investigated the possible role of gangliosides in the osteoblast differentiation of hADSCs and hDPSCs. First, we investigated characterization of hADSCs and hDPSCs using FACS analysis. Mesenchymal stem cell specific markers, CD44 and CD105, were expressed but not hematopoietic markers, CD45 and CD117 in both of hADSCs and hDPSCs. High-performance thin-layer chromatography analysis showed that increased gangliosides were associated with differentiation of hADSCs and hDPSCs into osteoblasts. RT-PCR analysis confirmed that osteoblast specific genes, ALP, BMP-2, collagen were expressed in differentiated osteoblasts, however, the another osteoblast specific gene, osteocalcin, was not expressed. When hADSCs and hDPSCs were cultured under osteoblast-differentiation conditions, alkaline phosphatase (ALP) activity was increased in comparison to hADSCs and hDPSCs. Furthermore, specifically both ALP activity and ganglioside expression increased more in hDPSCs-derived osteoblasts than hADSCs-derived osteoblasts. These results suggest that gangliosides play a more important role in regulating the osteoblast-differentiation of hDPSCs compared to hADSCs.

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Effects of gangliosides on the differentiation of human mesenchymal stem cells into osteoblasts by modulating epidermal growth factor receptors

Cited by 44 publications

References 17 publications

Stem Cell Glycolipids

Stem Cell Glycolipids

Erlotinib prevents experimental metastases of human small cell lung cancer cells with no epidermal growth factor receptor expression

Comparison of ganglioside expression between human adipose- and dental pulp-derived stem cell differentiation into osteoblasts

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