Effects of Gaseous Ozone Treatment on Bone Regeneration in Femoral Defect Model in Rats

Duman, İbrahim; Davul, Serkan; Gökçe, Hasan; Gönenci, Ramazan; Özden, Raif; Uruç, Vedat

doi:10.2485/jhtb.26.7

Cited by 10 publications

(10 citation statements)

References 29 publications

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“…Duman et al have stated that O3 improved bone regeneration in a femoral defect model in rats. The physiological effect attributed to Ozone Is that O3 improved blood circulation, differentiation, angiogenesis and finally fracture healing 47 . Philippou has stated that IGF-1 is important in regeneration, hypertrophy, proliferation and differentiation of skeletal muscles.…”

Section: Discussionmentioning

confidence: 99%

Intra Articular Ozone Modulates Inflammation and Has Anabolic Effect on Knee Osteoarthritis: IL-6 and IGF-1 as Pro-Inflammatory and Anabolic Biomarkers

Fernández-Cuadros¹,

Pérez-Moro²,

Albaladejo-Florín³

et al. 2021

Preprint

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Objectives: 1) to demonstrate the anti-inflammatory and anabolic effect of Ozone by determining in serum samples the biochemical levels of IL-6 and IGF-1 in knee osteoarthritis (OA) patients in a real world in Rehabilitation Setting; 2) to evaluate clinical effectiveness by Visual Analog Scale (VAS) and WOMAC scale, and biochemical effect by C-reactive protein (CRP), uric acid and erythrocyte sedimentation rate (ESR). Material and methods: 65 patients with knee OA Kellgren Lawrence (KL) grade 2 or more were analyzed in a retrospective observational study. The study run from January 2018 to September 2021. Inclusion criteria: a) patients 18 years or older; b) with knee OA KL 2º or more; c) biochemical analysis before-and-after treatment; d) pain more than 3 on VAS. Exclusion Criteria: a) previous knee surgery; b) favism; c) pregnancy; d) any other disease that originates lack of collaboration for infiltration. Primary Outcome variables: a) IL-6; b) IGF-1 in diabetes mellitus (DM)/obese and non-DM/non-obese patients; both before-and-after Ozone treatment. Secondary Outcome variables: a) CRP, b) ESR, c) uric acid, d) VAS pain, e) WOMAC pain, function and stiffness. Ozone protocol consisted of 4 sessions (once a week) of an intra-articular infiltration of 20 mL (20µg/mL concentration) of a gas mixture of Oxygen-Ozone 95-5% (produced by Ozone generator Ozonosan-α Plus ®). For biochemical evaluation, SNIBE MAGLUMI ™ IL-6 (CLIA) and SNIBE MAGLUMI ™ IGF-1 (CLIA) kits were used. CRP and uric acid were analyzed by Abbott Alinity c kit; and ESR was evaluated by DIESSE VES MATIC CUBE 30. Results: There is a linear correlation between age and OA severity. IL-6 decreased both in DM and non-DM patients and in all OA KL grades (from 2.7 to 1.59 pg/mL). IGF-1 decreased in total group (OA + DM + obesity) from 112.09 to 107.19 ng/mL. When only knee OA patients were analyzed, Ozone improved IGF-1 levels (from 100.17 to 102.03 ng/mL). Ozone decreased CRP, ESR, uric acid, and improved VAS pain, WOMAC pain, function and stiffness (p<0.05). Conclusions: Ozone is a valid option for the management of knee osteoarthritis in real world Rehabilitation Setting, because of its anti-inflammatory, metabolic and anabolic properties. Ozone downregulates pro-inflammatory IL-6 cytokine. Ozone has a metabolic/hypoglycemic effect on obese/diabetic knee osteoarthritis patients by reducing IGF-1. Ozone has an anabolic effect on non-diabetic/non-obese patients by improving IGF-1. Ozone reduces other biomarkers of inflammation (CRP, ESR and uric acid) and improves, pain, function and quality of life.

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Section: Discussionmentioning

confidence: 99%

Intra Articular Ozone Modulates Inflammation and Has Anabolic Effect on Knee Osteoarthritis: IL-6 and IGF-1 as Pro-Inflammatory and Anabolic Biomarkers

Fernández-Cuadros¹,

Pérez-Moro²,

Albaladejo-Florín³

et al. 2021

Preprint

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“…In addition, it can stimulate immune responses and blood circulation. (7,8) Ozone is popular with its anti-inflammatory and analgesic action; it helps in the synthesis of biologically active substances such as interleukins, leukotrienes and prostaglandins which is valuable in reducing inflammation and pain. Furthermore, it has been shown to be a powerful and reliable anti-microbial agent against bacteria, fungi, protozoa, and viruses.…”

Section: The Effect Of Ozone Gel On Bone Regeneration Following Enucl...mentioning

confidence: 99%

The Effect of Ozone Gel on Bone Regeneration Following Enucleation of Maxillary Cysts

Mnasour

2022

Egyptian Dental Journal

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Introduction: Reconstruction of bony defects represents a challenging problem for the surgical community. ozone therapy seems to be a promising future in different dental modalities that may be effective in treating bony defects. Objectives:The aim of this study was to assess the effect of topical ozone gel application on bone regeneration following enucleation of maxillary cysts. Materials and methods:After surgical enucleation of odontogenic maxillary cystic lesions in sixteen patients, eight bony defects were grafted with ozone gel; the second group served as controls. Computed multi-slice CT scans were obtained immediately after surgery, -6 and -9months post-surgery to assess the decrease in bone defect size and density of newly formed bone. Results:The study group showed significant increase in bone density of newly formed bone at 6& 9 months postsurgical (p= 0.039, 0.042) and significant decrease in bone defect size as it recorded a mean percentage of residual area of bone defect in axial cuts 39.46± 9.17, 22.52 ± 7.02 while control group recorded a mean of 64.08 ± 9.88, 49.74 ± 10.96 at 6& 9months respectively.Conclusions: Ozone gel can be considered a healing biomaterial, as it features all the necessary parameters permitting bone regeneration without any adverse reaction. It gives a predictable clinical and radiographic evidence of bone formation and faster healing than control group.

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“…Clinically, the importance of ossification and fracture healing is well known in rats. It has been reported that exposure to ozone application provides good healing in diabetes (15), oral mucositis (16), surgical treatment of peri-implantitis (17), bone regeneration (18,19), and osteogenesis with calvarial defects in experimental models (20). Many rodent experimental animals, including rats, have been used to design bone defect models (21)(22)(23)(24).…”

Section: Introductionmentioning

confidence: 99%

Effects of alloplastic graft material combined with a topical ozone application on calvarial bone defects in rats

et al. 2020

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Background:The present study presents the evaluation of the damage in the bone tissue resulting from a calvarial defect in rats and the efficiency of exposure to an ozone application with an alloplastic bone graft on the calvarial bone damage. Materials and methods:Wistar male rats (n = 56) were divided into four groups: a control group (n = 14), defect and ozone group (n = 14), defect and graft group (n = 14), and defect, graft, and ozone group (n = 14). Under anaesthesia, a circular full-thickness bone defect was created in all groups, and the experimental groups were further divided into two sub-groups, with seven rats in each group sacrificed at the end of the 4 th and 8 th weeks. Bone samples were dissected, fixed in 10% formalin solution, and decalcified with 5% ethylene-diaminetetraacetic acid (EDTA). After the routine follow-up on tissues, immunostaining of osteopontin and osteonectin antibodies was applied to sections and observed under a light microscope. Results:The control group exhibited osteopontin and osteonectin expression in fibroblasts and inflammatory cells at the end of the 4 th week with an acceleration at the 8 th week. Ozone administration elucidated new trabecular bone formation by increasing osteoblastic activity.Lastly, our observations underscore that a combination of allograft and ozone application increased the osteoblast, osteocyte, and bone matrix development at the 4 th and 8 th weeks. Conclusions:Exposure to an ozone application with an alloplastic bone graft on calvarial bone damage may induce osteoblastic activity, matrix development, mature bone cell formation, and new bone formation in rats.

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Effects of Gaseous Ozone Treatment on Bone Regeneration in Femoral Defect Model in Rats

Cited by 10 publications

References 29 publications

Intra Articular Ozone Modulates Inflammation and Has Anabolic Effect on Knee Osteoarthritis: IL-6 and IGF-1 as Pro-Inflammatory and Anabolic Biomarkers

Intra Articular Ozone Modulates Inflammation and Has Anabolic Effect on Knee Osteoarthritis: IL-6 and IGF-1 as Pro-Inflammatory and Anabolic Biomarkers

The Effect of Ozone Gel on Bone Regeneration Following Enucleation of Maxillary Cysts

Effects of alloplastic graft material combined with a topical ozone application on calvarial bone defects in rats

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