Effects of GB virus-C/hepatitis G virus on hepatitis B and C viremia in multiple hepatitis virus infections

“…Serum HCV RNA was assayed by reverse transcription (RT)-PCR with primers from the most conserved 5 0 untranslated region of the viral genome [Kao et al, 1998]. To avoid false-positive results, the methods described by Kwok and Higuchi [1989] to prevent cross contamination were applied.…”

Section: Detection Of Hcv Rnamentioning

confidence: 99%

Sequence analysis of pre‐S/surface and pre‐core/core promoter genes of hepatitis B virus in chronic hepatitis C patients with occult HBV infection

Kao

Chen

Lai

et al. 2002

Journal of Medical Virology

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Although occult hepatitis B virus (HBV) infection in individuals without detectable hepatitis B surface antigen (HBsAg) may occur and has been reported to be common in patients with chronic hepatitis C, the related molecular mechanisms remain unknown. With the polymerase chain reaction, serum HBV DNA was sought in 100 HBsAg-negative patients with chronic hepatitis C virus (HCV)-infection. In those with occult HBV infection, possible genomic variability of HBV was evaluated by amplification and direct sequencing of pre-S, surface, and pre-core/core promoter genes. In total, 10 of the 100 patients (10%) had detectable serum HBV DNA, documenting an occult HBV infection. A deletion mutant in the pre-S gene was found in one patient and mutations of the a determinant of HBsAg were observed in 2. In addition, a novel core promoter mutant (a dinucleotide substitution: T-to-C at nucleotide 1,802 and T-to-G at nucleotide 1,803, T1802C/T1803G) was found frequently in patients with occult HBV infection as compared to sex- and age-matched HBsAg-positive patients (80 vs. 10%, P < 0.001). In conclusion, the data suggest occult HBV infection is not uncommon in chronic hepatitis C patients in Taiwan, and a novel core promoter mutant may be associated with the absence of circulating HBsAg in these patients.

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“…Serum HBV DNA level was quantified by a branched DNA (bDNA) signal amplification assay (Hepatitis B Viral DNA, Chiron, Emeryville, CA) as described previously [Kao et al, 1998]. The HBV DNA quantification range of the bDNA assay is 2.5 ∼ 17,700 pg/mL.…”

Section: Quantitation Of Hbv Dna and Hcv Rnamentioning

confidence: 99%

TT virus infection in patients with chronic hepatitis B or C: Influence on clinical, histological and virological features

Kao

Chen

et al. 2000

J. Med. Virol.

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Concomitant infection with TT virus and hepatitis B virus (HBV) or hepatitis C virus (HCV) is common. However, the effect of TTV infection on chronic hepatitis B or C is unknown. The prevalence of TTV infection, the effect of TTV infection on the clinical, histological and virological features of patients with chronic hepatitis B or C, and the influence of TTV infection on the HCV response to interferon alfa therapy were studied. A total of 100 asymptomatic hepatitis B surface antigen carriers, 220 patients with HBV-related chronic liver diseases, and 110 patients with chronic hepatitis C treated with interferon alfa (3 million units subcutaneously three times a week for 24 weeks) were enrolled. Serum HCV RNA and serum TTV DNA were detected by the polymerase chain reaction (PCR). Serum HBV DNA and serum HCV RNA level were quantified by branched DNA assays. Infection with TTV was detected in 21.5% of HBV carriers and 37% of HCV carriers. TTV infection had little effect on the clinicopathological course of chronic HBV infection. In chronic hepatitis C, clinical features, histological severity, serum HCV RNA levels, and the response to interferon alfa therapy did not differ between those with and without TTV infection. The loss of serum TTV DNA did not correlate with the biochemical response as did in the loss of serum HCV RNA. In conclusion, TTV infection is found frequently in patients with chronic hepatitis B or C in Taiwan; however, coinfection with TTV does not affect the clinicopathological course of chronic hepatitis B or C and the response to interferon alfa therapy.

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Effects of GB virus-C/hepatitis G virus on hepatitis B and C viremia in multiple hepatitis virus infections

Cited by 12 publications

References 14 publications

GB virus C: Insights into co-infection

GB virus C: Insights into co-infection

Sequence analysis of pre‐S/surface and pre‐core/core promoter genes of hepatitis B virus in chronic hepatitis C patients with occult HBV infection

TT virus infection in patients with chronic hepatitis B or C: Influence on clinical, histological and virological features

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