Effects of genetic strain and holding facility on the characteristics of alkaline phosphatase and brush border enzymes in silver perch (Bidyanus bidyanus)

Abstract

The activity of the enzymes located in the digestive tract (pyloric caeca and intestine) in two strains and F1 crosses of the Australian freshwater fish silver perch [Murray River (M) and Cataract Dam (C)] was evaluated. The effect of the fish holding system (cages or ponds) on the activity of alkaline phosphatase (AP) and the brush border enzymes: maltase, leucine amino peptidase (LAP) and γ‐glutamyl transferase (γ‐GT) was examined. The enzymatic activity was tested in three intestinal sections: the pyloric caeca, upper and lower intestine. All the tested enzyme activities were influenced by an interaction between the intestinal section and the strain or cross of silver perch. For fish maintained in ponds, there were two distinct enzymatic activity patterns, one for the pure strains (C × C and M × M) and one for the crosses (C × M and M × C). Significantly higher (P<0.05) enzymatic activities of the crosses compared with the pure strains were particularly noticeable in the pyloric caeca. In fish held in cages, there was a significantly higher (P<0.05) enzymatic activity in the pyloric caeca of the C × C strain. The total enzymatic activities in the fish reared in the ponds were significantly higher (P<0.05) than those in the fish held in cages. The results of the present investigation confirm that the different genetic background of the silver perch in the Murray River and in the Cataract Dam influences the activity of the brush border digestive enzymes. This activity also varies between maintenance systems.

Development of digestive enzyme activity in larvae of spotted sand bass Paralabrax maculatofasciatus. 1. Biochemical analysis

“…Similar results have been reported for other species such as S. maximus (Cousin et al 1987) and D. labrax (Cahu and Zambonino-Infante 1997). The pattern of activity coincided with that of other brushborder enzymes which should indicate a maturation of enterocytes (Zambonino-Infante and Cahu 2001; Hakim et al 2007), while the clear drop in all activity from 12 dah onwards may be related to the onset of stomach activity.…”

Dietary preference and digestive physiology of plankti-benthivorous fishes inhabiting mudflats of Indian Sundarban estuaries

“…Likewise in the present study also, four gobiid species (G. giuris, T. buchanani, P. novemradiatus and T. vagina) were found in proximity in the dendrogram when overall digestive enzyme patterns were considered. Influence of the genetic traits on the activities of brush border enzymes was demonstrated in the crosses of Oreochromis mossambicus (Peters, 1852) and O. aureus (Steindacher, 1864) (Hakim et al 2006) and in the silver perch Bidyanus bidyanus (Mitchell, 1838) (Hakim et al 2007). Furthermore, the activities of digestive enzymes were also influenced by many other factors such as the ages of the fishes (Kuźmina 1996), temperature and season and the composition of their diets (Zambonino-Infante and Cahu 2001).…”

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“…Their results showed that the activities of digestive enzymes correlated more strongly with phylogeny rather than with the fish's natural diets. Influence of the genetic strains on the activities of brush border enzymes was demonstrated in the crosses of Oreochromis mossambicus and O. aureus (Hakim et al, 2006) and in the silver perch Bidyanus bidyanus (Hakim et al, 2007). Furthermore, the activities of digestive enzymes were also influenced by many other factors such as the ages of the fishes (Kuźmina, 1996), temperature and season (Kuźmina et al, 1996b) and the composition of their diets (Zambonino Infante and Cahu, 2001).…”