Effects of geometrical confinement in membrane pores on enzyme-based layer-by-layer assemblies

Wong, Diana Guadalupe Ramirez; Coelho-Diogo, Cristina; Aimé, Carole; Bonhomme, Christian; Jonas, Alain M.; Demoustier‐Champagne, Sophie

doi:10.1016/j.apsusc.2015.02.123

Cited by 13 publications

(8 citation statements)

References 59 publications

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“…Membrane substrates, from the family of polymeric materials, could facilitate analyte-surface interaction purely by their porous nature and desirable surface chemical compositions [15]. The modified ELISA methods have previously been developed on porous filter-membranes as an alternative to the conventional assay [4].…”

Section: Introductionmentioning

confidence: 99%

A novel approach for application of nylon membranes in the biosensing domain

Farahmand

Ibrahim

Hosseini

et al. 2015

Applied Surface Science

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Section: Introductionmentioning

confidence: 99%

A novel approach for application of nylon membranes in the biosensing domain

Farahmand

Ibrahim

Hosseini

et al. 2015

Applied Surface Science

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“…28 Furthermore, Ramirez-Wong et al used 1 H signals to determine the ratio of lysozyme to chitosan in a deposited layered conjugate. 12 Very recently, MAS NMR has been successfully employed to characterize a covalently immobilized enzymatic system consisting of a model enzyme α-chymotrypsin, mesoporous silica as the matrix, and (3-glycidyloxypropyl)trimethoxysilane (GLYMO) as the covalent linker. 30 However, this natural abundance MAS NMR study of covalently immobilized chymotrypsin was hampered by poor NMR sensitivity, making it impossible to obtain detailed structural and dynamic information from the immobilized enzyme.…”

Section: ■ Introductionmentioning

confidence: 99%

“…Natural abundance silica-entrapped lysozyme 13 C signals were also detectable using DNP enhancement . Furthermore, Ramirez-Wong et al used 1 H signals to determine the ratio of lysozyme to chitosan in a deposited layered conjugate …”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, solid-state NMR has been successfully employed for studying complex biological systems that are not accessible by X-ray crystallography or solution-state NMR . MAS NMR has been used previously to study a variety of immobilized enzymes and supports. − However, most of these research studies were focused mainly on studying the support systems and/or the biochemical characterization of the biocatalytic system. There have also been quite extensive high-resolution MAS NMR studies of proteins and peptides involved in biomineralisation, adsorbed on the surfaces of the inorganic materials whose growth they control. − However, there have so far only been a few studies of signals from enzyme molecules immobilized on supports as biocatalysts.…”

Section: Introductionmentioning

confidence: 99%

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High-Resolution Structural Characterization of a Heterogeneous Biocatalyst Using Solid-State NMR

et al. 2016

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Solid-state magic-angle spinning (MAS) NMR spectroscopy was employed to investigate structural detail in an enzyme, human carbonic anhydrase II (hCA II) in uniformly 15 N and selectively (15 N leucine) enriched states, covalently immobilized on epoxyfunctionalized silica. The immobilized hCA II retained 71% of its specific enzymatic activity when compared to the free enzyme in solution. Based on the one-and two-dimensional 1 H, 13 C, 15 N and 29 Si MAS NMR spectra, chemical shift assignments could be obtained from the silica support, covalent linker and the immobilized enzyme. The successful covalent immobilization of the enzyme on epoxy-silica was confirmed by the appearance of signals from the aromatic and carbonyl groups in the immobilized enzyme in addition to signals from the modified support. Most notably, our MAS NMR results suggest that the covalent immobilization of the hCA II on epoxy-silica does not significantly affect the structural integrity of the protein.

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“…It is based on alternative deposition of oppositely charged species, encompassing polyelectrolytes, nanoparticles, nanotubes, quantum dots, dyes, or proteins . These coatings can be applied to any shape and size of materials, and films with well-defined architectures, composition, and chemical functionalities can be constructed. , Additionally, these films possess tunable properties since their morphology, internal molecular structure, and thickness can be controlled by varying the deposition conditions, such as ionic strength pH and concentration of the polyelectrolyte solution …”

Section: Introductionmentioning

confidence: 99%

Evaluation of Adhesion Forces for the Manipulation of Micro-Objects in Submerged Environment through Deposition of pH Responsive Polyelectrolyte Layers

et al. 2015

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Optimization of surface treatment for reversible adhesion of micro-objects in liquid environment for the need in microassembly processes is presented. A spherical borosilicate probe and planar oxidized silicon wafer substrates were modified by deposition of pH sensitive polyelectrolyte films through layer-by-layer technique. Branched polyethylenimine (b-PEI) and poly(sodium styrenesulfonate) (PSS) were deposited in alternating manner on surfaces, and the influence of polyelectrolyte concentration, pH of deposition, and number of layers on the adhesion were successively examined. The multilayer buildup was followed by optical reflectometry (OR) and dissipative quartz crystal microbalance (QCM-D). The adhesion forces were monitored in aqueous environment at variable pH values by colloidal probe AFM microscopy. The thermodynamic work of adhesion was derived from the pull-off forces by using the Johnson-Kendall-Roberts (JKR) model and compared to the work of adhesion determined from contact angle measurements. It was found out that they correlate well, however, the values accessed from JKR model were underestimated, which was attributed mainly to the effect of surface roughness. Obtained results have demonstrated that it is possible to achieve repeatable reversible adhesion with the change of pH of submerged environment by appropriately tailoring the surface properties and therefore the prevailing surface forces.

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Effects of geometrical confinement in membrane pores on enzyme-based layer-by-layer assemblies

Cited by 13 publications

References 59 publications

A novel approach for application of nylon membranes in the biosensing domain

A novel approach for application of nylon membranes in the biosensing domain

High-Resolution Structural Characterization of a Heterogeneous Biocatalyst Using Solid-State NMR

Evaluation of Adhesion Forces for the Manipulation of Micro-Objects in Submerged Environment through Deposition of pH Responsive Polyelectrolyte Layers

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