Effects of ginkgo biloba on in vitro osteoblast cells and ovariectomized rat osteoclast cells

Oh, Seung Min; Kim, Ha Ryong; Chung, Kyu Hyuck

doi:10.1007/s12272-001-1144-z

Cited by 20 publications

(17 citation statements)

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“…In vitro studies demonstrated a proliferation of positive dose-response of EGb on the culture of osteoblastic-like cells -MC3T3-E1 -and suggested that the antioxidant activity of the extract protected osteoblasts from cellular damage and death when they were exposed to the action of free radicals (Haramaki et al, 1994;Brayboy et al, 2001). Cells from the Saos-2 (human osteoblast-like cells) lineage exposed to EGb increased the production of alkaline phosphatase, thus promoting osteoblast mineralization (Oh et al, 2008). Furthermore, quercetin and kaempferol, two of the major components of EGB, exert a potent inhibitory effect on bone resorption inducing apoptosis of osteoclasts (Wattel et al, 2003) and can stimulate osteoblastic activity (Prouillet et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Radiographic evidence of mandibular osteoporosis improvement in Wistar rats treated with Ginkgo biloba

Lucinda

Oliveira

Salvador

et al. 2009

Phytotherapy Research

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Section: Introductionmentioning

confidence: 99%

Radiographic evidence of mandibular osteoporosis improvement in Wistar rats treated with Ginkgo biloba

Lucinda

Oliveira

Salvador

et al. 2009

Phytotherapy Research

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“…The occurrence of osteoporosis is associated with decreased osteoblastic activity and increased osteoclastic activity [Riggs and Melton, 1988]. SaOS-2 cells have been used to assess the effect of herbal compounds on the proliferation, differentiation and matrix mineralization of osteoblastic cells Huh et al, 2006;Oh et al, 2008]. Multiple signaling pathways that affect osteoblast function converge to and are being coordinated by Runx2.…”

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confidence: 99%

Effects of Cissus quadrangularis on the proliferation, differentiation and matrix mineralization of human osteoblast like SaOS‐2 cells

Muthusami

Krishnamoorthy

Vignesh

et al. 2011

J of Cellular Biochemistry

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Osteoporosis is a public health problem which is associated with significant morbidity and mortality. The repair of bone defect is still a big challenge for orthopedic surgeons. Traditional use of Cissus quadrangularis (C. quadrangularis) in the treatment of bone disorders has been documented. The present study was employed to delineate the effects of ethanolic extract of C. quadrangularis on the proliferation, differentiation and matrix mineralization of human osteoblast like SaOS-2 cells. Lactate dehydrogenase assayed in the conditioned medium of control and C. quadrangularis treated cells did not differ significantly indicating that ethanolic extract of C. quadrangularis is nontoxic to osteoblastic cells. [(3)H] Thymidine incorporation assay revealed that C. quadrangularis treatment has increased the DNA synthesis of human osteoblastic SaOS-2 cells indicating increased proliferation of these cells. The data on alizarin red and ALP staining revealed increased matrix mineralization of human osteoblast like SaOS-2 cells. The study also revealed that the anabolic actions of ethanolic extract of C. quadrangularis in human osteoblast like cells are mediated through increased mRNA and protein expression of Runx2, a key transcription factor involved in the regulation of bone matrix proteins. Chromatin immunoprecipitation analysis revealed increased transcriptional activity of Runx2 on the promoter of osteocalcin after C. quadrangularis treatment. These results indicate positive regulation of C. quadrangularis on the proliferation, differentiation, and matrix mineralization of human osteoblast like SaOS-2 cells.

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“…al., 1994). Além de aumentar os níveis de fosfatase alcalina óssea (Oh et al, 2008;Prouillet et al, 2004).…”

Section: Resultsunclassified

“…O EGb, in vitro, causou aumento significativo de fosfatase alcalina óssea, promovendo mineralização pelos osteoblastos (Oh et al, 2008). Estudo recente com administração oral do EGb 761 demonstrou aumento da massa óssea de ratas com osteoporose induzida por ovariectomia (Trivedi et al, 2009).…”

Section: Introductionunclassified

Efeito do extrato de Ginkgo biloba L., Ginkgoaceae, na osteoporose induzida em ratas Wistar

Lucinda¹,

Vieira²,

Salvador³

et al. 2010

Rev. bras. farmacogn.

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RESUMO:Este trabalho investigou os efeitos do tratamento por vinte dias com extrato de Ginkgo biloba (EGb) na osteoporose induzida por glicocorticóides. Foram utilizadas 36 ratas divididas em seis grupos (n=6): Controle, osteoporose, controle positivo, EGb1 (14 mg EGb/mg/kg/dia), EGb2 (28 mg EGb/kg/dia) e EGb3 (56 mg EGb/kg/dia). Os tratamentos foram realizados por vinte dias, após a indução da osteoporose. Após a eutanásia foram removidos o fêmur e a mandíbula de todos os animais. A mandíbula esquerda foi radiografada digitalmente para avaliação da cortical e do suporte ósseo periodontal (SOP). A análise histomorfométrica foi realizada no fêmur e mandíbula direitos. O grupo controle foi comparado ao grupo osteoporose (Teste t de Student) e os demais grupos foram submetidos a ANOVA, seguido do teste post-hoc de Dunnett. Houve redução significava do SOP mesial, percentual ósseo alveolar (POA) mandibular, percentual ósseo trabecular (POT) do fêmur no grupo osteoporose. Houve aumento do SOP mesial no grupo controle positivo, EGb2 e EGb3. O POA da mandíbula e o POT do fêmur aumentaram nos grupos EGb2 e EGb3. O EGb nas doses de 28 mg/kg e 56 mg/kg recuperou de forma significativa o SOP mesial, o POA da mandíbula e o POT do fêmur. Unitermos:Ginkgo biloba, osteoporose, radiografia, histomorfometria, alendronato de sódio, ratos.ABSTRACT: "Effect of the aqueous extract of Ginkgo biloba L., Ginkgoaceae, in induced osteoporosis in Wistar rats." The objective of this study was to investigate the effect of a 20 day treatment with extract of Ginkgo biloba (EGb) in glucocorticoid-induced-osteoporosis. 36 rats were divided into six groups (n=6): control, osteoporosis, positive control, EGb1 (14 mg EGb/kg/day), EGb2 (28 mg EGb/kg/day) and EGb3 (56 mg EGb/kg/day). Treatments were conducted for twenty days, after osteoporosis was induced. Following euthanasia the femur and mandible of all animals were removed. The left mandible was radiographed to evaluate the cortical and the periodontal bone support (PBS). The histomorphometric analysis was performed on the right mandible and the right femur. The control group was compared with the osteoporosis group (Student's t-test). The other groups were analyzed through ANOVA test followed by Dunnett post-hoc test. There was a significantly reduction in the mesial PBS, in the percentage of the alveolar bone (PAB) of the mandible and percentage of the trabecular bone (PTB) of the femur in the osteoporosis group. There was an increase in the mesial PBS in the positive control group, EGb2 and EGb3. The PAB of the mandible and the PTB of the femur increased in the EGb2 and EGb3 groups. The EGb in the 28 mg/kg and 56 mg/kg doses were effective to increase the mesial PBS, the PAB of the mandible and the PTB of the femur.Keywords: Ginkgo biloba, osteoporosis, radiography, histomorphometric, sodium alendronate, rats. INTRODUÇÃOA osteoporose é caracterizada por uma redução na quantidade de tecido ósseo por unidade de volume (Hallworth, 1998) e por uma deteriorização da microarquitetura deste tecido...

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Effects of ginkgo biloba on in vitro osteoblast cells and ovariectomized rat osteoclast cells

Cited by 20 publications

References 64 publications

Radiographic evidence of mandibular osteoporosis improvement in Wistar rats treated with Ginkgo biloba

Radiographic evidence of mandibular osteoporosis improvement in Wistar rats treated with Ginkgo biloba

Effects of Cissus quadrangularis on the proliferation, differentiation and matrix mineralization of human osteoblast like SaOS‐2 cells

Efeito do extrato de Ginkgo biloba L., Ginkgoaceae, na osteoporose induzida em ratas Wistar

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