Effects of ginseng saponins isolated from Red Ginseng roots on burn wound healing in mice

Abstract

We recently demonstrated that ginsenoside Rb1 (C54H92O23, molecular weight 1108) isolated from ginseng, when intravenously infused into rats with permanent middle cerebral artery occlusion, reduced cerebral infarct volume and ameliorated place navigation disability of the animals, through an anti‐apoptotic action and possibly promotion of vascular regeneration. To investigate the ginsenoside Rb1‐mediated vascular regeneration in vivo in a more easily accessible experimental systems, we made a burn wound on the backs of mice and topically applied either Vaseline (vehicle) alone or Vaseline containing low doses of ginsenoside Rb1 to the wound. Surprisingly, we found that ginsenoside Rb1 at low concentrations (100 pg g−1, 1 pg g−1 and 10 fg g−1 ointment) exhibited the strongest burn wound‐healing action. Furthermore, ginsenoside Rb1 (100 fg–1 ng per wound) increased neovascularization in the surrounding tissue and production of vascular endothelial growth factor (VEGF) and interleukin (IL)‐1β from the burn wound, compared to those mice with burn wounds treated with vehicle alone. In human keratinocyte cultures (HaCaT cells), ginsenoside Rb1 (100 fg ml−1 to 1 ng ml−1) enhanced VEGF production induced by IL‐1β and expression of hypoxia‐inducible factor (HIF)‐1α. These findings suggest that the promotion of burn wound healing by ginsenoside Rb1 might be due to the promotion of angiogenesis during skin wound repair via the stimulation of VEGF production, through the increase of HIF‐1α expression in keratinocytes, and due to the elevation of IL‐1β resulting from the macrophage accumulation in the burn wound. British Journal of Pharmacology (2006) 148, 860–870. doi:

Cosmetic Potential of Enzymatic Treated Ginseng Leaf

“…ginseng has been shown to possess a wide range of biological and pharmacological activities and is used as a general tonic in traditional oriental medicine to increase vitality, health, and longevity, especially in older persons. Previous research has reported that ginseng has several beneficial effects on human and animal skin [3][4][5][6][7]. Kim et al [36] reported that topical applications of total ginseng saponins (10 pg or 100 ng/mouse) and ginsenoside Rb1 (100 fg, 10 pg, or 1 ng/mouse) isolated from red ginseng significantly inhibited increases in skin thickness and wrinkle formation and reduced skin elasticity induced by long-term UVB irradiation.…”

Alternative animal model for studies of total skin thickness burns

“…Several works using aluminum coupled to the soldering iron have been presented as an effective method for the reproduction of the experimental model of third degree burns 8,9,[16][17][18][19] , but there is a great discrepancy in the temperature used, which may vary from 100ºC to 200ºC, in addition to the time of application that varies between 5 to 30 seconds. Caliari-Oliveira et al…”

“…Subsequently, the process of burning the skin of the animal was started through a 3 cm diameter circular aluminum plate coupled to a soldering iron, with a total weight of 190 grams [8][9][10] (Figure 1), heated at a temperature of 150 °C, controlled with the aid of a thermostat ( Figure 2) [10][11][12] . The plate was pressed in the dorsal region of the animals in three different times, being for G5: 5 seconds, G10: 10 seconds and G15: 15 seconds.…”

Preventive and Therapeutic Roles of Ginseng - Focus on Colon Cancer

“…Hamed Jafari Vayghan 1 , Sevda Saleh Ghadimi 1 , Ali Reza Nourazarian 2,3 * (Yue et al, 2006) 4-reduce inflammatory responses, (Oh GS et al, 2004;Kimura et al, 2006) According to previous studies ginseng can be beneficial in the treatment and prevention of colorectal cancer (Yun and Choi 1995;Yun et al, 2001;Yun 2003a). The aim of this study was to review relevant literature on ginseng effects on colorectal cancer.…”