Effects of glucocorticoid on human dermal papilla cells in vitro

Choi, Sejong; Cho, A-Ri; Jo, Seong Jin; Hwang, Sungjoo; Kim, Kyu Han; Kwon, Oh Sang

doi:10.1016/j.jsbmb.2012.11.009

Cited by 24 publications

(28 citation statements)

References 22 publications

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“…1c, d). Downregulation of VEGF and HGF is consistent with the previous findings obtained for Dex-treated dermal papilla cells (Choi et al 2013). Upregulation of MMP7 and DKK-1 is consistent with the previous findings obtained for Dex-treated gingival fibroblasts (Cury et al 2007) and osteoblasts (Ohnaka et al 2004), respectively.…”

Section: Resultssupporting

confidence: 92%

“…Genes differentially upregulated and downregulated (twofold) by Dex are shown in Table S1 and Table S2, respectively. Candidate genes (MMP1, MMP3, MMP7, MMP12, TIMP4, VEGF, HGF, ROR2, FRZB, and DKK-1) were selected based on the relevance of regulation of their expression by Dex in other cell types (Ohnaka et al 2004;Cury et al 2007;Choi et al 2013) and were validated by real-time PCR analysis (Fig. 1c, d).…”

Section: Resultsmentioning

confidence: 99%

“…A recent in vitro study showed that treatment with dexamethasone (Dex), a synthetic GC, inhibits the proliferation of the dermal papilla cells of human hair follicle, causes translocation of the glucocorticoid receptor (GR) into the nucleus, and decreases the expression of growth factors required for hair growth; this suggests that GC-induced changes in the dermal papilla cells may contribute to stress-associated disturbance of hair growth (Choi et al 2013). These findings prompted us to investigate whether stress-associated periodontal disturbance can be attributed to GC-induced changes in the periodontal ligament stem cells (PDLSC) that are present in the connective tissue of the periodontal ligament and are believed to be the most promising candidate Mi Hee Kwack and Young Kwan Sung contributed equally to this work.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effects of dexamethasone, a synthetic glucocorticoid, on human periodontal ligament stem cells

Choi¹,

Park

Kwack

et al. 2015

Naunyn-Schmiedeberg's Arch Pharmacol

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Recent studies have showed that psychosocial stress causes elevated secretion of cortisol, the principal glucocorticoid (GC), and thus increases the extent of periodontal breakdown. In this study, we investigated whether stress-associated periodontal disturbance may be due to GC-induced changes in the periodontal ligament stem cells (PDLSCs), one of the most promising candidates for periodontal tissue regeneration. Our results in this study showed that dexamethasone (Dex) treatment causes the translocation of the glucocorticoid receptor (GR) into the nucleus and increases the expression of many genes, including dickkopf-1 (DKK-1) in PDLSCs. ELISA showed that DKK-1 is secreted from PDLSCs in response to Dex treatment. The GR antagonist RU486 attenuated the Dex-inducible DKK-1 messenger RNA (mRNA) expression. DKK-1 inhibited the growth of PDLSCs and suppressed Wnt-mediated activation of β-catenin signaling in PDLSCs. Our results strongly suggest that stress-associated periodontal disturbance may be due to GC-induced changes in the activity of PDLSCs via DKK-1 expression and might provide a possible explanation for the deteriorating effect of stress on periodontal breakdown.

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Section: Resultssupporting

confidence: 92%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of dexamethasone, a synthetic glucocorticoid, on human periodontal ligament stem cells

Choi¹,

Park

Kwack

et al. 2015

Naunyn-Schmiedeberg's Arch Pharmacol

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“…Glucocorticoids (GC) are synthesized mostly in the adrenal gland in response to stressful conditions, and mediate cellular damage such as hair loss. Treatment of DPCs with Dexamethasone resulted in decreased cell viability, reduced VEGF and HGF secretion, ER alpha levels and increased expression of aromatase mRNA . Geranium sibiricum extract alleviated the damaging effects in DPCs induced by Dexamethasone .…”

Section: Protective Effect In Hair Growth Against Stressmentioning

confidence: 99%

Review of Hair Follicle Dermal Papilla cells asin vitroscreening model for hair growth

Madaan

Verma

Singh

et al. 2018

Intern J of Cosmetic Sci

127

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Hair disorders such as hair loss (alopecia) and androgen dependent, excessive hair growth (hirsutism, hypertrichosis) may impact the social and psychological well-being of an individual. Recent advances in understanding the biology of hair have accelerated the research and development of novel therapeutic and cosmetic hair growth agents. Preclinical models aid in dermocosmetic efficacy testing and claim substantiation of hair growth modulators. The in vitro models to investigate hair growth utilize the hair follicle Dermal Papilla cells (DPCs), specialized mesenchymal cells located at the base of hair follicle that play essential roles in hair follicular morphogenesis and postnatal hair growth cycles. In this review, we have compiled and discussed the extensively reported literature citing DPCs as in vitro model to study hair growth promoting and inhibitory effects. A variety of agents such as herbal and natural extracts, growth factors and cytokines, platelet-rich plasma, placental extract, stem cells and conditioned medium, peptides, hormones, lipid-nanocarrier, light, electrical and electromagnetic field stimulation, androgens and their analogs, stress-serum and chemotherapeutic agents etc. have been examined for their hair growth modulating effects in DPCs. Effects on DPCs' activity were determined from untreated (basal) or stress induced levels. Cell proliferation, apoptosis and secretion of growth factors were included as primary end-point markers. Effects on a wide range of biomolecules and mechanistic pathways that play key role in the biology of hair growth were also investigated. This consolidated and comprehensive review summarizes the up-to-date information and understanding regarding DPCs based screening models for hair growth and may be helpful for researchers to select the appropriate assay system and biomarkers. This review highlights the pivotal role of DPCs in the forefront of hair research as screening platforms by providing insights into mechanistic action at cellular level, which may further direct the development of novel hair growth modulators.

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“…Upon interaction with GC, the glucocorticoid receptor (GR) translocates into the nucleus, where it mediates a variety of effector mechanisms . A recent study has shown that GR is expressed in human dermal papilla (DP) cells and that Dex inhibits the proliferation of DP cells and decreases the expression of growth factors required for hair growth . These results strongly suggest that stress‐associated suppression of hair growth may be due to GC‐induced changes in DP cells.…”

Section: Introductionmentioning

confidence: 99%

Dickkopf‐1 is involved in dexamethasone‐mediated hair follicle regression

Kwack

Lee

Seo

et al. 2017

Experimental Dermatology

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The stress-related neurohormones including glucocorticoids (GCs) are secreted by hair follicles (HFs), and GCs suppress murine hair growth in vivo. In this study, we found that dexamethasone (Dex), a synthetic GC, increased the expression of dickkopf-1 (Dkk1), a known catagen inducer, in dermal papilla (DP) cells, but not in follicular keratinocytes. The neutralizing Dkk1 antibody significantly attenuated the Dex-induced inhibition of human hair shaft elongation. In addition, the neutralizing Dkk1 antibody delayed Dex-induced catagen in mice.Collectively, our data strongly suggest that stress-related neurohormones cause DP cells to secrete Dkk1, thereby leading to stressassociated disturbances in hair growth. | BACKGROUNDIncreased secretion of cortisol, the principal glucocorticoid (GC) hormone, is triggered in response to psychosocial stress on the hypothalamic-pituitary-adrenal (HPA) axis. Cortisol is also secreted by human hair follicles (HFs), which act as a functional equivalent of the HPA axis.[1] Dexamethasone (Dex), a synthetic GC, induces catagen, also known as the HF regression phase, in mice. [2,3] upon interaction with GC, the glucocorticoid receptor (GR) translocates into the nucleus, where it mediates a variety of effector mechanisms. [4] A recent study has shown that GR is expressed in human dermal papilla (DP) cells and that Dex inhibits the proliferation of DP cells and decreases the expression of growth factors required for hair growth. [5] These results strongly suggest that stressassociated suppression of hair growth may be due to GC-induced changes in DP cells. Furthermore, we have previously shown that dickkopf-1 (Dkk1) promotes catagen by inducing apoptosis in follicular keratinocytes as well as by suppressing Wnt/β-catenin signalling.[6] | QUESTION ADDRESSEDFirst, we tested whether Dex induced the secretion of Dkk1 from follicular cells. Next, we investigated whether Dkk1 was involved in Dex-mediated HF regression. | EXPERIMENTAL DESIGNExperiments were performed as previously described, [7] and the detailed methods are included in Data S1. | RESULTSWe found that GR is strongly expressed in inner and outer root sheath (ORS) of hair follicles. We also found relatively weak expression of GR in matrix, DP and dermal sheath (n=5; Fig. S1). We observed that DKK1 mRNA is upregulated in DP cells (n=3) in response to Dex as examined by RT-PCR and real-time PCR ( Figure 1A,C). The time-course study showed that DKK1 expression is upregulated in DP cells as early as 6 hours after a 100 nM Dex treatment (Fig. S2). Data from ELISA (n=3) also showed that the concentration of Dkk1 in the DP conditioned medium is increased in response to Dex ( Figure 1E). These results are consistent with those obtained in previous studies that reported an increase in Dkk1 expression in osteoblasts and periodontal ligament stem cells treated with Dex. [8,9] Furthermore, Ru486, a synthetic GR antagonist, significantly attenuated Dex-induced DKK1 mRNA expression in DP cells (n=3; Figure 1F,G), demonstrating that ...

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Effects of glucocorticoid on human dermal papilla cells in vitro

Cited by 24 publications

References 22 publications

Effects of dexamethasone, a synthetic glucocorticoid, on human periodontal ligament stem cells

Effects of dexamethasone, a synthetic glucocorticoid, on human periodontal ligament stem cells

Review of Hair Follicle Dermal Papilla cells asin vitroscreening model for hair growth

Dickkopf‐1 is involved in dexamethasone‐mediated hair follicle regression

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