Effects of glycinin and β-conglycinin on enterocyte apoptosis, proliferation and migration of piglets

Abstract

Glycinin and b-conglycinin have been identified as major food/feed allergens. But effects of glycinin and b-conglycinin on enterocyte migration in piglets are scare. Fifteen weanling (7.0690.18 kg) General No. 1 barrows, weaned at 28 days, were used. The piglets were randomly allotted to three (A, B and C) treatments with five replicates. The piglets in the A group (control group) were fed diets without ingredients originating from leguminous products, while the piglets in the B or C groups were fed the diets containing purified glycinin or b-conglycinin, which replaced protein in Group A by 4%. All the experimental periods were followed for 7 days. Five-micrometre thick sections of small intestinal tissue were stained with the TUNEL method to assess apoptotic activity, and with Ki-67 immunohistochemistry to assess cellular proliferation. The results indicated that glycinin or b-conglycinin increased proliferative index and apoptotic index in duodenum for piglets (P B0.05).

Effects of β‐conglycinin on growth performance, antioxidant capacity and intestinal health in juvenile golden crucian carp,Carassius auratus

“…In addition, FE and PE were significantly decreased by 40–80 g/kg dietary β‐conglycinin. These findings were supported by the results in other studies on terrestrial animals and fish (Hao et al, ; Ma, ; Zhang et al, ; Zhao, Qin, Sun, Zhang, & Wang, ). In turbot and crab studies, feeding dietary β‐conglycinin showed growth performance was significantly decreased.…”

Soybean β-Conglycinin Induces Inflammation and Oxidation and Causes Dysfunction of Intestinal Digestion and Absorption in Fish

“…Thus, to define mechanisms whereby excessive soybean induces growth depression in animals; this study investigated the role of purified soybean β-conglycinin, separating its effects from the effects of other anti-nutritional factors. Firstly, the present study observed that fish fed diet with β-conglycinin exhibited poor weight gain, which was also observed in piglets [7], [8] and rats [60]. However, survival was not influenced by the dietary treatments.…”

“…Among these anti-nutrients, β-conglycinin was suggested as being one of the key anti-nutritional factors [6]. A recent study has reported that β-conglycinin reduced weight gain in pigs [7]. However, little attention has been given to the potential negative effects of β-conglycinin on the growth, digestive and absorptive ability of fish.…”

β-Conglycinin Reduces the Tight Junction Occludin and ZO-1 Expression in IPEC-J2

“…Transient hypersensitivity to soybean antigens could lead to villus atrophy, crypt hypertrophy [14–16,39]. In addition, β-conglycinin may accelerate the enterocyte apoptosis and the relative enterocyte migration rate for piglets in our previous study [18]. The above literature provides evidence that β-conglycinin could induce the low enterocyte activity.…”

“…Soybean allergies usually cause inflammatory disorders in the small intestine, characterized by villous atrophy and hyperplasia in the crypt, as well as accelerated enterocyte proliferation, apoptosis and migration [14–18]. However, so far, there have been few studies into the effects of β-conglycinin on the intestinal barrier permeability of piglets.…”