Effects of glyphosate on egg incubation, larvae hatching, and ovarian rematuration in the estuarine crab <i>Neohelice granulata</i>

Avigliano, Luciana; Alvarez, Natalia; Loughlin, Camila Mac; Rodríguez, Enrique M.

doi:10.1002/etc.2635

Cited by 27 publications

(14 citation statements)

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“…Despite its low toxicity to arthropods, glyphosate residues may have sublethal effects and affect their fitness [14–18]. In the honeybee, glyphosate or Roundup formulation can induce an increase in lipid peroxidation, a decrease in β-carotene and derived antioxidants, and a reduction in acetylcholine esterase activity [13,19–21].…”

Section: Introductionmentioning

confidence: 99%

Glyphosate, but not its metabolite AMPA, alters the honeybee gut microbiota

et al. 2019

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The honeybee ( Apis mellifera ) has to cope with multiple environmental stressors, especially pesticides. Among those, the herbicide glyphosate and its main metabolite, the aminomethylphosphonic acid (AMPA), are among the most abundant and ubiquitous contaminant in the environment. Through the foraging and storing of contaminated resources, honeybees are exposed to these xenobiotics. As ingested glyphosate and AMPA are directly in contact with the honeybee gut microbiota, we used quantitative PCR to test whether they could induce significant changes in the relative abundance of the major gut bacterial taxa. Glyphosate induced a strong decrease in Snodgrassella alvi , a partial decrease of a Gilliamella apicola and an increase in Lactobacillus spp. abundances. In vitro , glyphosate reduced the growth of S . alvi and G . apicola but not Lactobacillus kunkeei . Although being no bee killer, we confirmed that glyphosate can have sublethal effects on the honeybee microbiota. To test whether such imbalanced microbiota could favor pathogen development, honeybees were exposed to glyphosate and to spores of the intestinal parasite Nosema ceranae . Glyphosate did not significantly enhance the effect of the parasite infection. Concerning AMPA, while it could reduce the growth of G . apicola in vitro , it did not induce any significant change in the honeybee microbiota, suggesting that glyphosate is the active component modifying the gut communities.

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Section: Introductionmentioning

confidence: 99%

Glyphosate, but not its metabolite AMPA, alters the honeybee gut microbiota

et al. 2019

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“…N. granulata and other crustacean species (24,25,(29)(30)(31)(32). The vitellogenin synthesis can certainly rapidly detected; as reported by Reddy Buchi et al (33), the expression of vitellogenin ARNm occurred as early as 3 hours after beginning the in vitro incubation of crab hepatopancreas.…”

Section: In Vitro Effects On Female Crabsmentioning

confidence: 85%

“…Moreover, by means of evaluating the incorporation of a radiolabeled amino acid to the ovarian proteins, it could be established that partial inhibition of the protein synthesis was caused by Roundup Ultramax ® [Figure 6 in (23), Figure 2A here], accounting for the decreased vitellogenin content observed in vitro. Short-term changes in vitellogenic protein content (either increase or decrease) have been also observed by effect of other pollutants, and even hormones, in several in vitro assays made on (24,25). In all cases, glyphosate concentrations are expressed as acid equivalents (a.e.…”

Section: In Vitro Effects On Female Crabsmentioning

confidence: 99%

The Effect of Glyphosate on the Reproduction of Estuarine Crabs: Neohelice granulata as a Study Model

et al. 2021

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This review summarizes the bulk of evidence about the effect of glyphosate, both technical and formulated, on the ovarian maturation of Neohelice granulata female crabs, as well as the effects of glyphosate on sperm production in males of the same species. After long-term in vivo assays, made during the 3-month pre-reproductive period of this species, both formulated and technical glyphosate were able to produce a significant incidence of oocyte reabsorption in the ovary, together with a concomitant decreased of vitellogenin content, at concentrations ranging from 0.2 to 1 mg/L. Despite this, after 32-day in vivo assays, glyphosate stimulated oocyte growth, in terms of a higher percentage of vitellogenic oocytes, suggesting that glyphosate could be acting as an endocrine disruptor. In vitro assays made with isolated ovarian pieces showed a decrease of vitellogenin content, in correlation with lower protein synthesis, although some advance in maturation was observed in the histological analysis. In male crabs exposed in vivo to both technical and formulated glyphosate at 1 mg/L, several reproductive imbalances were noted, such as a significant decrease of the sperm count, abnormal spermatophores, and possible disrupting effects of glyphosate on the androgenic gland.

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“…Além do comprometimento reprodutivo, os herbicidas a base de glifosato podem alterar a atividade do ácido retinóico, hormônio importante durante o desenvolvimento embrionário (Paganelli et al, 2010). De fato, vários trabalhos mostram que após exposição a estes herbicidas, diversas espécies animais incluindo peixes (Paganelli et al, 2010;Zebral et al, 2017), ave (Paganelli et al, 2010), anfíbio (Paganelli et al, 2010) e crustáceo (Avigliano et al, 2014) apresentaram alterações embrionárias semelhantes ao fenótipo de desregulação do ácido retinóico, como alterações craniofaciais (Lohnes et al, 1994) e redução/ausência de olhos (Lopez e Carrasco, 1992). Além disto, é sabido que áreas rurais onde os herbicidas a base de glifosato são extensivamente utilizados vem sofrendo aumento no número de bebes nascidos com alterações embrionárias.…”

Section: Desregulação Endócrinaunclassified