Effects of gonadotrophin treatment in vivo on testicular function in immature rhesus monkeys (Macaca mulatta)

Arslan, Muhammad; Zaidi, P.; Akhtar, F. Bint; Amin, S.; Rana, Tanu; Qazi, Mahmood Husain

doi:10.1111/j.1365-2605.1981.tb00731.x

Cited by 9 publications

(4 citation statements)

References 20 publications

(17 reference statements)

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“…The present finding that a significant increase in testicular volume occurred as early as Day 4 of treatment with the recombinant gonadotropins, either alone or in combination, is consistent with the previously reported rapid testicular growth in response to gonadotropin treatment in juvenile monkeys [21]. Thus, the Sertoli and Leydig cells of the juvenile primate testis must express functional FSH and LH receptors that are coupled to appropriate signal transduction pathways.…”

Section: Discussionsupporting

confidence: 91%

Pulsatile Stimulation with Recombinant Single Chain Human Luteinizing Hormone Elicits Precocious Sertoli Cell Proliferation in the Juvenile Male Rhesus Monkey (Macaca mulatta)1

Ramaswamy¹,

Plant²,

Marshall³

2000

Biology of Reproduction

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In this study, we determined the relative role of LH and FSH in initiating the pubertal proliferation of Sertoli cells in primates. Sixteen juvenile male rhesus monkeys (Macaca mulatta) bearing venous catheters received intermittent intravenous infusions of single chain human LH (schLH) or recombinant human FSH (rhFSH) or a combination of both for 11 days. The schLH infusion elicited a physiological testosterone response. On Day 11, monkeys were castrated, and one-half of a testis was fixed in Bouin's fluid. Infusion of the gonadotropins, either alone or in combination, effected a significant increase in testicular weight, seminiferous cord diameter, and the number of Sertoli cells per testis (schLH, 295 +/- 46 x 10(6); rhFSH, 342 +/- 64 x 10(6); LH+FSH, 298 +/- 26 x 10(6) versus vehicle, 204 +/- 26 x 10(6)). The latter finding indicated that LH, in addition to FSH, plays a critical role in the initiation of the pubertal proliferation of Sertoli cells in primates. Moreover, combined gonadotropin treatment led to the appearance of germ cells as mature as early primary spermatocytes, indicating that initiation of spermatogenesis had been set in motion. Because the duration of hormone stimulation was only 11 days, the latter result suggests that Leydig and Sertoli cells of the juvenile monkey testis can immediately transduce a gonadotropin signal to the germ cell.

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Section: Discussionsupporting

confidence: 91%

Pulsatile Stimulation with Recombinant Single Chain Human Luteinizing Hormone Elicits Precocious Sertoli Cell Proliferation in the Juvenile Male Rhesus Monkey (Macaca mulatta)1

Ramaswamy¹,

Plant²,

Marshall³

2000

Biology of Reproduction

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“…Reports such as that of Greep (1937) indicated enlargement of gonads of immature animals after treatment with non-human pituitary fractions containing FSH. The marked enlargement of seminiferous tubules and Sertoli cell cytoplasm noted in immature rhesus monkeys following oFSH or PMSG treatment (Arslan et al, 1981 ) clearly indicates a lack of species specificity in hormone interaction, consistent with our observations (Text- fig. 4).…”

Section: Discussionsupporting

confidence: 91%

Identification of specific FSH binding sites in the testes of adult monkeys of different species

Berman¹,

Sairam²

1984

Reproduction

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The interaction of 125I-labelled hFSH with primate testicular tissue from 4 species of adult monkeys (Macaca mulatta, M. nemestrina, M. fascicularis and Papio cynocephalus) was investigated. 125I-labelled hFSH binding to a particulate fraction (P1, 40 000 g) of frozen testes was highly specific and saturable. Displacement curves generated using the P1 fraction of testes from the 4 species and 125I-labelled hFSH and unlabelled FSH were very similar. The binding of FSH to the monkey testicular receptor was not species specific because purified FSH from heterologous species such as horse, sheep, pig and rat were very effective in competing with 125I-labelled hFSH for binding. The equine FSH was about 10 times more active than hFSH in this respect. Similarly, 125I-labelled ovine FSH bound as well as labelled hFSH to the testes fractions of all 4 monkey species. In marked contrast to the high binding of 125I-labelled hFSH, binding of 125I-labelled hCG with rhesus monkey testis homogenates and P1 fractions was very low. The FSH receptor in the adult rhesus monkey testis was present in much larger quantity than the LH receptor and was more readily detectable. Our studies show that frozen primate testis can be utilized for investigating testicular-FSH interactions.

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“…In our in vivo experiments, the increase in testosterone synthesis by the testis seems to be a consequence of the endogenous release of gonadotrophins from repeated stimulation with the LHRH analogue. A comparable stimulation of testicular androgenesis has previously been demonstrated by administering exogenous gonadotrophins chronically, to immature monkeys (Arslan & Qazi 1976;Arslan et al 1981). In the present study, the in vitro refractoriness of the testicular tissue from untreated animals and the responsiveness of the tissue from LHRH-agonist-primed monkeys, to hCG, demonstrate a functional differentiation of this tissue following LHRH analogue treatment.…”

Section: Discussionsupporting

confidence: 59%

Effect of an LHRH Analogue on Testicular Function in the Immature Monkey (Macaca mulatta)

1982

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Response of the immature monkey testis to an LHRH analogue, D-Ser-(TBu)6-EA10-LHRH, has been assessed by measuring testicular androgen production in the immature rhesus monkey. Administration of the analogue (2.5 micrograms X 2 daily) for 5 days induced a significant increase in testicular testosterone compared with untreated animals. The response of the control and the LHRH-agonist treated monkey testis to gonadotrophin (hCG) was assessed by in vitro androgen production. Whereas the testicular tissue from LHRH analogue primed monkeys responded to in vitro stimulation of hCG, no significant increase in androgen production was observed when testicular tissue from untreated animals was incubated with the gonadotrophin. The present data suggest a functional differentiation of the immature monkey testis following the LHRH analogue treatment.

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Effects of gonadotrophin treatment in vivo on testicular function in immature rhesus monkeys (Macaca mulatta)

Cited by 9 publications

References 20 publications

Pulsatile Stimulation with Recombinant Single Chain Human Luteinizing Hormone Elicits Precocious Sertoli Cell Proliferation in the Juvenile Male Rhesus Monkey (Macaca mulatta)1

Pulsatile Stimulation with Recombinant Single Chain Human Luteinizing Hormone Elicits Precocious Sertoli Cell Proliferation in the Juvenile Male Rhesus Monkey (Macaca mulatta)1

Identification of specific FSH binding sites in the testes of adult monkeys of different species

Effect of an LHRH Analogue on Testicular Function in the Immature Monkey (Macaca mulatta)

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