Effects of Granulocyte-Macrophage Colony-Stimulating Factor in Burn Patients

Cioffi, William G.; Burleson, David G.; Jordan, Bryan S.; Becker, William K.; McManus, William F.; Mason, Arthur D.; Pruitt, Basil A.

doi:10.1001/archsurg.1991.01410250080013

Cited by 40 publications

(15 citation statements)

References 35 publications

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“…This approach has been proposed previously. Specifically, treatment with granulocyte colony-stimulating factor or granulocyte-macrophage colony-stimulating factor (GM-CSF) after burn injury has been shown to have positive effects on survival and immune cell function (10,17), including improved T-cell proliferation and IL-2 production in a rat burn model (29).…”

Section: Discussionmentioning

confidence: 99%

Stimulation of Hematopoiesis by the Fms-Like Tyrosine Kinase 3 Ligand Restores Bacterial Induction of Th1 Cytokines in Thermally Injured Mice

Toliver‐Kinsky

Chen²,

Herndon

et al. 2003

Infect Immun

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Section: Discussionmentioning

confidence: 99%

Stimulation of Hematopoiesis by the Fms-Like Tyrosine Kinase 3 Ligand Restores Bacterial Induction of Th1 Cytokines in Thermally Injured Mice

Toliver‐Kinsky

Chen²,

Herndon

et al. 2003

Infect Immun

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“…Both G-CSF and GM-CSF improved immune function in animal models of burn-associated sepsis (17,18). Treatment of burn patients with GM-CSF improved neutrophil activities in vitro, but effects on the incidence of infections or mortality were not reported (19). It is likely that the most widely effective therapies will be those that impact numerous effector cells, which may require combinatorial therapies.…”

mentioning

confidence: 99%

Enhancement of Dendritic Cell Production by Fms-Like Tyrosine Kinase-3 Ligand Increases the Resistance of Mice to a Burn Wound Infection

Toliver‐Kinsky

Cui

Murphey

et al. 2005

The Journal of Immunology

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Fms-like tyrosine kinase-3 ligand (Flt3L) is a hemopoietic cytokine that stimulates the production of dendritic cells. This study evaluated the ability of Flt3L-enhanced dendritic cell production to increase the resistance of mice to a burn wound infection with Pseudomonas aeruginosa, a common source of infections in burn patients that have impaired immunity and are susceptible to opportunistic microorganisms. Treatment of mice with Flt3L for 5 days caused a significant increase in dendritic cell numbers in the spleen and significantly increased survival upon a subsequent burn wound infection. Improved survival in Flt3L-treated mice was associated with limited bacterial growth and spread within the burn wounds and a decrease in systemic dissemination of P. aeruginosa. Resistance to burn wound infection could also be conferred to recipient mice by the adoptive transfer of dendritic cells that had been isolated from spleens of Flt3L-treated mice. Adoptive transfer of the same number of splenic dendritic cells from nontreated mice did not confer resistance to burn wound infection. These data indicate that Flt3L can increase the resistance of mice to a P. aeruginosa burn wound infection through both stimulation of dendritic cell production and enhancement of dendritic cell function.

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“…They found external used rhGM-CSF can inhibit the wound healling delay caused by bacteria infection. Cioffi [6] et al use rhGM-CSF intravenously for burn patients in an randomly-controlled observation. They found that after 4 day's administration white blood count in treatment group was significant higher than that in control group, and blood bacterial culture presented negative in treatment group.…”

Section: Discussionmentioning

confidence: 99%

A novel formulation of recombinent human grannulocyte/macrophage colony-stimulating factor hydro-gel, its characterizations and its therapeutic effect on burns in animal model

Zhou

Han

Ding

et al. 2011

2011 International Conference on Remote Sensing, Environment and Transportation Engineering

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Objective In order to observe the effect of recombinant human granulocyte/macrophage colony-stimulating factor (rhGM-CSF) gel for external use on animal model of burn. Methods rhGM-CSF gel for external use was prepared, and its quality control and stability at different temperatures were investigated extensively based on methods describe in China Pharmacopeia (Edition 2005), including appearance, activity, pH, homogeneity and microbial limit. The rat model of burn was established with electric flatiron and treated with blank gel matrix, basic fibroblast growth factor (bFGF) spraying and rhGM-CSF gel for external use at high (15μ μg/cm) and low (7.5μg/cm 2 ) doses, respectively. Evaluate the curative effect of rhGM-CSF gel for external use by observing the healing time, infection of burn surface and death rate of model rats. Results Formulations and preparation procedures for rhGM-CSF gel for external use was simple and easy to scale up. The stability results of rhGM-CSF gel for external use were 3 months at 37 , 6 months at 25 and 2 years at 4 , respectively. The time for healing of burn surface of model rats treated with rhGM-CSF gel for external use at both high and low doses was significantly shorter than that treated with blank gel matrix. There was no infection and death in rats with burn. The time for hair growth after healing of burn surface of mice treated with rhGM-CSF gel for external use was significantly shorter than that with blank gel matrix. Conclusion The rhGM-CSF gel for external use showed a good quality control, a long-term stability and a curative effect on animal model of burn.

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Effects of Granulocyte-Macrophage Colony-Stimulating Factor in Burn Patients

Cited by 40 publications

References 35 publications

Stimulation of Hematopoiesis by the Fms-Like Tyrosine Kinase 3 Ligand Restores Bacterial Induction of Th1 Cytokines in Thermally Injured Mice

Stimulation of Hematopoiesis by the Fms-Like Tyrosine Kinase 3 Ligand Restores Bacterial Induction of Th1 Cytokines in Thermally Injured Mice

Enhancement of Dendritic Cell Production by Fms-Like Tyrosine Kinase-3 Ligand Increases the Resistance of Mice to a Burn Wound Infection

A novel formulation of recombinent human grannulocyte/macrophage colony-stimulating factor hydro-gel, its characterizations and its therapeutic effect on burns in animal model

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