Summary• This study examined the effects of warming, elevated atmospheric CO 2 and cutting regimen on the growth of Phalaris aquatica cv. Holdfast swards.• Six temperature gradient tunnels ( TGT ) were used to manipulate both air temperature and atmospheric CO 2 concentrations (ambient and 750 ppm). Within each tunnel, there were three temperature treatments: no warming, constant warming of +3.0 ° C and a daytime warming of 2.2 ° C combined with a night-time warming of 4.0 ° C and two defoliation frequencies.• Averaged across 20 months of growth, there was a positive effect of elevated atmospheric CO 2 (+11%), no effect of either warming treatment and a negative effect of frequent cutting ( − 19%) on total above ground biomass production. The responses to all treatments, however, were strongly seasonal.• Positive responses to CO 2 were statistically significant only in the spring, when plant growth was strongest. No evidence was found that high night-time warming had different effects on plant growth and plant responses to elevated atmospheric CO 2 , than constant warming.