Effects of Green Liquor Pretreatment on the Chemical Composition and Enzymatic Hydrolysis of Several Lignocellulosic Biomasses

Wang, Wang‐Xia; Meng, Xin; Min, Douyong; Song, Junlong; Jin, Yongcan

doi:10.15376/biores.10.1.709-720

Cited by 14 publications

(11 citation statements)

References 22 publications

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“…Lignocellulosic biorefineries can produce monosaccharides by different technologies [17,18]. During a biorefinery process, pretreatment is an essential step required to disrupt the indurative structure of biomass for subsequent enzymatic hydrolysis [19,20]. One pretreatment technology, autohydrolysis, is remarkably green and effective from both a technological and an economic perspective.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Carbon Quantum Dot Nanoparticles Derived from Byproducts in Bio-Refinery Process for Cell Imaging and In Vivo Bioimaging

et al. 2019

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The carbon quantum dot (CQD), a fluorescent carbon nanoparticle, has attracted considerable interest due to its photoluminescent property and promising applications in cell imaging and bioimaging. In this work, biocompatible, photostable, and sustainably sourced CQDs were synthesized from byproducts derived from a biorefinery process using one-pot hydrothermal treatment. The main components of byproducts were the degradation products (autohydrolyzate) of biomass pretreated by autohydrolysis. The as-synthesized CQDs had a size distribution from 2.0–6.0 nm and had high percentage of sp2 and sp3 carbon groups. The CQDs showed blue-green fluorescence with a quantum yield of ~13%, and the fluorescence behaviors were found to be stable with strong resistance to photobleaching and temperature change. In addition, it is found that the as-synthesized CQDs could be used for imaging of cells and tumors, which show potential applications in bioimaging and related fields such as phototherapy and imaging.

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Section: Introductionmentioning

confidence: 99%

Synthesis of Carbon Quantum Dot Nanoparticles Derived from Byproducts in Bio-Refinery Process for Cell Imaging and In Vivo Bioimaging

et al. 2019

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“…An example is pre‐treatment with green liquor, based on the adaptation of the kraft pulping process used in pulp and paper mills . The delignification process promoted by the liquor is adapted for the production of lignocellulosic ethanol from the wood, instead of cellulose pulp production . This approach could increase the flexibility of industrial plants, since they could switch to manufacturing the most attractive product, according to the current economic scenario .…”

Section: Introductionmentioning

confidence: 99%

“…This approach could increase the flexibility of industrial plants, since they could switch to manufacturing the most attractive product, according to the current economic scenario . It is possible that mills could be transformed into biorefineries, reducing initial investments and operating costs …”

Section: Introductionmentioning

confidence: 99%

Effect of phenolic compounds on bioconversion of glucose to ethanol by yeast Saccharomyces cerevisiae PE‐2

2018

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The bioconversion of lignocellulosic biomasses to ethanol consists of three main steps: pre‐treatment, enzymatic hydrolysis, and fermentation by microorganisms. Most pre‐treatments induce the formation of substances with a potential inhibitory effect on the microbial metabolism, which may hinder the process. The objective of this study was to investigate the behaviour of Saccharomyces cerevisiae PE‐2 yeast in the presence of four phenolic compounds (vanillin, syringaldehyde, syringic acid, and acetosyringone) resulting from the alkaline pre‐treatment of eucalyptus with green liquor. Eucalyptus is a fast‐growing tree, widely exploited by the paper and pulp industry in Brazil. It was observed that yeast growth and glucose assimilation were affected to an extent directly proportional to the initial amount of vanillin in the medium. Syringaldehyde in a concentration of 1.0 g/L had an adverse effect on the fermentation parameters related to ethanol production, which exhibited a decrease of approximately 50 %. When syringic acid and acetosyringone were used, although the cell growth and glucose consumption profiles were similar to those of the control, they inhibited the conversion at concentrations of 0.1 and 1.0 g/L.

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“…Lignocellulosic biomass material was a poplar pulp, which was pretreated with green liquor as described elsewhere [40]. Poplar chips were collected from the north of Jiangsu Province, China.…”

Section: Methodsmentioning

confidence: 99%

Comparison of the Interaction Modes Between Cellulase and Bovine Serum Albumin /Lignosulfonate /Polyethylene Glycol in Promoting Lignocellulose Saccharification

Wang

Lan

Guo

et al. 2021

Preprint

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Background: Bovine serum albumin (BSA), polyethylene glycol (PEG) and lignosulfonate (LS) have been extensively employed as synergistic agents in lignocellulose saccharification, albeit it has not been fully understood how they interact with enzymes from the perspectives of molecular interactions. Herein, we attempted to unveil the promotion mechanisms of BSA, PEG and LS for lignocellulose saccharification from the perspective of their respective interaction with cellulase using Quartz Crystal Microbalance with Dissipation monitoring (QCM-D), Surface Plasmon Resonance (SPR), and Small Angle X-ray Scattering (SAXS) to investigate their respective interaction and the complex formation. In the meanwhile, we compared the effects of adding these additives into the enzymatic hydrolysis of pure cellulose (Avicel) and green liquor-pretreated lignocellulose (GL).Results: The results showed that BSA and LS could bind to cellulase to form complexes, whereas PEG did not. However, PEG had a high affinity to lignin or lignin derivatives. In term for Avicel and GL substrates, the results showed that BSA and PEG promoted the enzymatic hydrolysis of both substrates, while LS had a promoting effect for GL only and inhibited some extent for Avicel. Conclusions: This study showed that synergistic agents of LS, BSA, and PEG have different interaction modes with cellulase. BSA and LS form complexes with cellulase and the formed complexes prevent from nonproductive binding by residue lignin; whereas PEG prevents from nonproductive binding by forming a thin layer on residue lignin which actually serve as steric hindrance. This investigation will help us to understand the sophisticated interactions among the components in the complicated enzymatic system, especially the interactions between enzymes and synergistic agents. It will be helpful in the design and utilization of synergistic additives in the lignocellulose biorefinery process as well.

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Effects of Green Liquor Pretreatment on the Chemical Composition and Enzymatic Hydrolysis of Several Lignocellulosic Biomasses

Cited by 14 publications

References 22 publications

Synthesis of Carbon Quantum Dot Nanoparticles Derived from Byproducts in Bio-Refinery Process for Cell Imaging and In Vivo Bioimaging

Synthesis of Carbon Quantum Dot Nanoparticles Derived from Byproducts in Bio-Refinery Process for Cell Imaging and In Vivo Bioimaging

Effect of phenolic compounds on bioconversion of glucose to ethanol by yeast Saccharomyces cerevisiae PE‐2

Comparison of the Interaction Modes Between Cellulase and Bovine Serum Albumin /Lignosulfonate /Polyethylene Glycol in Promoting Lignocellulose Saccharification

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