Effects of growth hormone treatment on the expression of somatotropic axis genes in the skeletal muscle of lactating Holstein cows

Castigliego, Lorenzo; Armani, Andrea; Grifoni, Goffredo; Rosati, R.; Mazzi, M.; Gianfaldoni, Daniela; Гуиди, Алессандра

doi:10.1016/j.domaniend.2010.02.001

Cited by 12 publications

(22 citation statements)

References 47 publications

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“…Carcass conformation, stature, chest width, body depth, rump width, carcass and cull cow weight were all associated with SNPs in two genes while carcass fat was associated with SNPs in all three genes. This emphasizes the key role of GH mediated via IGF-1 and GHR in postnatal growth in cattle (Castigliego et al 2010). As previously reported, LD analysis within GH1, GHR and IGF-1 between SNPs exhibiting significant associations (Tables S1-S3) showed no evidence of high or even moderate LD between SNPs (Mullen et al 2010(Mullen et al , 2011aWaters et al 2011).…”

Section: Resultssupporting

confidence: 82%

Polymorphisms in genes of the somatotrophic axis are independently associated with milk production, udder health, survival and animal size in Holstein‐Friesian dairy cattle

Waters

Berry²,

Mullen³

2011

J Animal Breeding Genetics

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The somatotrophic axis consisting of pituitary-derived growth hormone and circulating insulin-like growth factor 1 has been well established as key regulators of animal health, metabolism, lactation, fertility, body composition and growth rate. The aim of this study was to simultaneously quantify the associations between SNPs in candidate genes of the somatotrophic axis (i.e., IGF-1, GH1 and GHR) with performance traits in Holstein-Friesian (HF) dairy cattle. Both novel SNPs identified previously by this group alongside other published SNPs within these genes were analysed for associations with performance in dairy cattle. Multiple regression analyses regressing genetic merit of up to 848 HF sires on novel SNPs (n = 76) and published SNPs (n = 33) were undertaken using weighted animal mixed linear models. Twenty-three SNPs were significantly associated with at least one of 18 traits analysed and involved in milk production, udder health, fertility and growth. Eight traits including milk fat composition, carcass conformation, stature, chest width, body depth, rump width, carcass and cull cow weight were independently associated with SNPs in two genes. Furthermore, for several traits including milk fat yield, somatic cell count, survival and carcass fat, SNPs in all three genes were independently associated with performance. Milk fat yield and carcass fat showed the highest number of independent associations across all three genes with five SNPs associated with both traits. The cumulative effects of the favourable alleles of all five SNPs across GH1, GHR and IGF-1 result in an increase of 5.9 kg and 28.6 units of milk fat and carcass fat, respectively. Cow survival was associated with a single SNP in each of the three genes with a cumulative allele effect of 1.5%. Independent effects of polymorphisms in GH1, GHR and IGF-1 reinforce the central role of the somatotrophic axis on animal development and performance.

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Section: Resultssupporting

confidence: 82%

Polymorphisms in genes of the somatotrophic axis are independently associated with milk production, udder health, survival and animal size in Holstein‐Friesian dairy cattle

Waters

Berry²,

Mullen³

2011

J Animal Breeding Genetics

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“…Consequently, gene expression of GHR in muscle was downregulated compared with control mice. In addition, Castigliego et al (2010) reported that expression of GHR was downregulated in bovine muscle after administration of recombinant bovine GH. The results of our correlation analysis support these findings (Dauncey et al, 1994;Katsumata et al, 2000;Spurlock et al, 2006) in that moderate negative associations were observed between expression of GHR and BW at slaughter, carcass weight, and LM area per kilogram of carcass weight.…”

Section: Discussionmentioning

confidence: 99%

Effect of sire breed and genetic merit for carcass weight on the transcriptional regulation of the somatotropic axis in longissimus dorsi of crossbred steers1

Keady

Kenny

Keane

et al. 2011

Journal of Animal Science

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The somatotropic axis plays an important role in postnatal growth, development, and differentiation of skeletal muscle. The aim of this study was to examine the effect of sire breed and sire EPD for carcass weight (EPD(cwt)) on the expression of components of the somatotropic axis in LM of beef cattle at slaughter. Crossbred Aberdeen Angus (AA; n = 17) and Belgian Blue (BB; n = 16) steers born to Holstein-Friesian dams and sired by bulls with either high (H) or low (L) EPD(cwt) were used in the study. Thus, there were 4 genetic groups [i.e., BBH (n = 8), BBL (n = 8), AAH (n = 8), and AAL (n = 9)]. Blood samples were collected via jugular venipuncture at regular intervals for analysis of plasma concentrations of IGF-1 and insulin. Total RNA was isolated from LM collected at slaughter, and the mRNA expression of IGF-1, IGF-2, their receptors (IGF-1R; IGF-2R), 6 IGFBP, acid labile subunit (ALS), and GH receptor (GHR) was measured by real-time reverse-transcription quantitative PCR. There was no effect of either sire breed or EPD(cwt) on concentrations of circulating IGF or insulin (P > 0.05). Gene expression of IGF-1R and IGFBP3 was upregulated in AA (P < 0.001) compared with BB, whereas IGF-1 was upregulated in H compared with L animals (P < 0.01). Correlation analysis indicated moderate positive associations between gene expression of IGFBP3 and IGF-1 (r = 0.54; P < 0.001) and IGF-1R (r = 0.48; P < 0.01). In addition, correlation analysis revealed that mRNA expression of IGFBP3 was moderately negatively associated with LM area per kilogram of carcass weight (r = -0.40; P < 0.05). Greater gene expression of IGF-1 and reduced transcript abundance of IGFBP3 in muscle may have a role in increased muscle growth potential in steers during the finishing period. These data will contribute to a better understanding of the molecular control of muscle growth at a tissue level in cattle.

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“…The insulin-like growth factor (IGF) system, consisting of 2 growth factors (IGF-I and IGF-II), 2 IGF receptors [IGF-IR and IGF-IIR/cation-independent mannose 6-phosphate receptor (M6PR)], and 7 IGF-binding proteins (IGFBP1-7), has been characterized as an important regulatory system for controlling tissue growth and development in vertebrates (Richards et al, 2005;Castigliego et al, 2010;Wu et al, 2011). IGF can regulate cellular proliferation and differentiation through the endocrine and auto/paracrine systems (Jones and Clemmons, 1995;Simmen et al, 1998;Liu and LeRoith, 1999;Xu et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Expression profile of insulin-like growth factor system genes in muscle tissues during the postnatal development growth stage in ducks

Song

Liu²,

Kou³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. Insulin-like growth factors (IGFs) are regulators that modulate the proliferation and differentiation of muscle tissues. We quantified the messenger RNA (mRNA) expression of IGF-I, IGF-II, and type I and II IGF receptors (IGF-IR and IGF-IIR) in muscle tissues including the breast, leg, and myocardium during an early postnatal development growth stage (post-hatching weeks 1-8) in ducks. The results showed a significant age-related change in mRNA in these muscle tissues. In breast muscle, the developmental expression of IGF-I and IGF-II was highest during week 1 but decreased quickly and maintained a relatively lower level. Leg muscle had the highest mRNA expression of IGF-I and IGF-II genes at week 3. In myocardial tissues, the expression level of IGF-IR and IGF-IIR genes exhibited a "rise-decline" developmental trend. The expression patterns of IGF-I/ IGF-IR and IGF-II/IGF-IIR were different between weeks 4 and 6. The same expression pattern was observed for IGF-I and IGF-IR; however, it was different from that observed for IGF-II and IGF-IIR. Our results showed a negative correlation between IGF-II mRNA expression and leg muscle weight at week 4 (P < 0.05). A negative correlation was also found between IGF-II mRNA expression and breast muscle weight (P < 0.01), and a positive correlation was found between IGF-IR expression and breast muscle weight. At week 6, a positive correlation was found between IGF-IR expression and breast muscle weight. However, at week 8, a negative correlation was found between IGF-IR expression and breast muscle weight. The results showed that the expression of IGF mRNA in duck tissues exhibits a specific developmental trend and an age-related pattern, suggesting that the regulation mechanism of these 4 genes in proliferation and differentiation of muscle tissues differed.

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Effects of growth hormone treatment on the expression of somatotropic axis genes in the skeletal muscle of lactating Holstein cows

Cited by 12 publications

References 47 publications

Polymorphisms in genes of the somatotrophic axis are independently associated with milk production, udder health, survival and animal size in Holstein‐Friesian dairy cattle

Polymorphisms in genes of the somatotrophic axis are independently associated with milk production, udder health, survival and animal size in Holstein‐Friesian dairy cattle

Effect of sire breed and genetic merit for carcass weight on the transcriptional regulation of the somatotropic axis in longissimus dorsi of crossbred steers1

Expression profile of insulin-like growth factor system genes in muscle tissues during the postnatal development growth stage in ducks

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