Effects of HA released calcium ion on osteoblast differentiation

Jung, Gil-Yong; Park, Yoon-Jeong; Han, Jung‐Suk

doi:10.1007/s10856-010-4011-y

Cited by 164 publications

(124 citation statements)

References 23 publications

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“…An et al demonstrated that increased concentrations of Ca 2+ ions had no effect on proliferation of human dental pulp cells, but led to changes in osteogenic differentiation [21]. Similarly, Jung et al demonstrated that osteoblast differentiation was enhanced by Ca 2+ released from hydroxyapatite [22]. These findings are contradictory to our observations, which indicate that a large increase in Ca 2+ concentration (the HCM and KMC-C extracts) has a cytotoxic effect on osteoblast cells in vitro.…”

Section: -contrasting

confidence: 99%

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Do novel cement-type biomaterials reveal ion reactivity that affects cell viability in vitro?

et al. 2013

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Calcium phosphate bioceramics have been studied as bone filler materials for years and have become a component of many commercial products. It is widely known that surface-reactive biomaterials may cause changes in the concentration of crucial ions in the surrounding environment, thereby affecting cell metabolism and viability. The aim of this study was to produce five cement-type biomaterials and characterize their phase composition using X-ray diffraction method, and porosity and pore size distribution using mercury intrusion porosimeter. We then evaluated ion interactions of the novel biomaterials with the surrounding environment (culture medium). A commercially available bone substitute, HydroSet™ (Stryker®), was used as a reference. MTT and NRU cytotoxicity tests were performed to assess the effect of changes in the concentration of crucial ions (calcium, magnesium, phosphate) on osteoblast metabolism and viability in vitro. Our study clearly indicated that various biomaterials demonstrated different ion reactivity and consequently may cause changes in ion concentration in the local environment. Critically low or high values of calcium, magnesium, and phosphate concentrations in the medium exerted cytotoxic effects on the cultured cells. Moreover, we discovered that the chemical composition of the culture medium had a substantial influence on ion interactions with biomaterials.

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Section: -contrasting

confidence: 99%

“…Interestingly, according to the available literature, increased levels of calcium ions are considered to promote osteogenic differentiation and mineralization [21,22]. An et al demonstrated that increased concentrations of Ca 2+ ions had no effect on proliferation of human dental pulp cells, but led to changes in osteogenic differentiation [21].…”

Section: -mentioning

confidence: 99%

Do novel cement-type biomaterials reveal ion reactivity that affects cell viability in vitro?

et al. 2013

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“…The ability to form a carbonated HA on the surface is considered to be the main aspect of CaP grafts` bioactivity [13]. The release of Ca has also positive effects on the differentiation of osteoblasts because it activates Ca-dependent protein kinases via Ca channels, leading to a higher expression of ALP, osteopontin and OC [57].…”

Section: Characterization and Preparation Of Synthetic Hamentioning

confidence: 99%

“…[54].The activation of runx2 as the most important pro-osteogenic differentiation factor via ERK1/2 pathways plays a central role in these processes [57]. In the case that other ions are a part of the HA lattice, it may have additional effects.…”

Section: Characterization and Preparation Of Synthetic Hamentioning

confidence: 99%

Molecular, Cellular and Pharmaceutical Aspects of Synthetic Hydroxyapatite Bone Substitutes for Oral and Maxillofacial Grafting

Götz¹,

Papageorgiou²

2017

CPB

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Bone grafts are widely used for augmentation procedures in oral and maxillofacial surgery, with autogenous bone being the gold standard. Recently, the focus of research has shifted towards synthetic bone substitutes, as no second surgery is needed and large quantities of graft can easily be provided. Within the broad range of bone substitutes, synthetic hydroxyapatite has drawn much attention, as they are considered to be biocompatible, non-immunogenic, osteoconductive and osteoinductive. Scope of this review is to summarize existing knowledge concerning the molecular, cellular and pharmaceutical aspects of synthetic bone substitutes for oral and maxillofacial grafting.

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“…In particular, enzyme-mediated hydroxyapatite-based biomaterials have been used in animal tests and have achieved successful outcomes. One possible reason for this is that enzyme-mediated hydroxyapatite is readily soluble to body fluids due to its low crystallinity, and the release of calcium ions may cause desirable effects, such as promoting the differentiation of osteoblastic cells as reported by Jung et al 98) Enzyme-mediated hydroxyapatite-based biomaterials may become the first successful example of practical application of this technique if the biological safety of the enzymes and reagents used is guaranteed.…”

Section: Concluding Remarks and Future Outlookmentioning

confidence: 99%

Enzyme-mediated synthesis of ceramic materials

Unuma

Matsushima

Kawai

2011

J. Ceram. Soc. Japan

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Some enzymes catalyze the formation of precipitants of metal ions, and some others directly interact with metal-containing precursors. Such enzymes allow the synthesis of inorganic solids under mild conditions. Due to the nature of enzymatic reactions, enzyme-mediated ceramic synthesis techniques can facilitate (1) site-selective ceramic deposition, (2) low temperature ceramic synthesis, (3) intact combination of ceramics with heat-sensitive materials, and sometimes (4) synthesis of metastable phases. The morphology of the resultant ceramics can be tailored in many ways by immobilizing enzymes onto various templates. So far, successful cases have been reported in preparing thin film coatings, hollow micro-or nanospheres, multi-layer stacking, nanotubes, 2D patterns, honeycombs, 3D replicas of biominerals, and so on. Enzyme-mediated ceramic synthesis provoked the emergence of a new type of processing technique which employs proteins or peptides as the mediators. Practical applications of enzyme-or peptide-mediated ceramics are on the horizon in many fields, such as biomaterials, enzyme immobilization supports, and enzyme sensors. This article presents a comprehensive survey of the recent advances of enzyme-mediated ceramic synthesis and related techniques as well as future outlook.

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Effects of HA released calcium ion on osteoblast differentiation

Cited by 164 publications

References 23 publications

Do novel cement-type biomaterials reveal ion reactivity that affects cell viability in vitro?

Do novel cement-type biomaterials reveal ion reactivity that affects cell viability in vitro?

Molecular, Cellular and Pharmaceutical Aspects of Synthetic Hydroxyapatite Bone Substitutes for Oral and Maxillofacial Grafting

Enzyme-mediated synthesis of ceramic materials

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