Effects of halothane reexposure in female mice and their offspring

Puig, N; Amerio, N; Piaggio, Eliane; Barragan, J.; Comba, J. O.; Elena, G

doi:10.1016/s0890-6238(99)00031-3

Cited by 9 publications

(4 citation statements)

References 37 publications

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“…Both lymphoproliferation and delayed type hypersensitivity reactions were conserved in halothane-treated animals. As regards toxicity, halothane-treated animals showed microscopic evidence of hepatotoxicity with diffuse metamorphosis, and diminished lipid peroxidation (Elena et al 1997, Puig et al 1999. Based on present results and our experience on single sevo urane anaesthesia (Puig et al 2002) we conclude that sevo urane is more innocuous to the immune system than halothane together.…”

Section: Discussionmentioning

confidence: 49%

Effects of repetitive sevoflurane anaesthesia on immune response, select biochemical parameters and organ histology in mice

et al. 2003

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SummaryAnimal and technical models often require repeated anaesthetic administrations for surgical procedures. As there is evidence for immunomodulatory effects of anaesthesia, the effects of repeated exposure to sevo urane anaesthesia on the immune response in mice were studied. Sevo urane was administered in vivo under conditions that simulate those in clinical procedures. Adult male mice were anaesthetized with 3% sevo urane in oxygen for 40 min weekly for 3 weeks. Untreated animals served as controls. After sevo urane anaesthesia, peripheral blood leukocyte counts, the composition and in vitro function of spleen cells (lymphocytes and macrophages) and the in vivo immune response to a conventional T-dependent antigen were assessed. In addition, liver, spleen and kidney histopathology and also hepatic and renal function were studied. Three days after the latest anaesthetic procedure, the absolute number of both leukocyte and lymphocyte counts were reduced in peripheral blood. Splenic cell composition (LB, LTCD3‡ , LTCD4 ‡ and LTCD8 ‡ ), macrophage function and the mitogen-induced lymphoprolipherative response were preserved. Yet, the in vivo humoral response to a conventional antigen was augmented following the antigenic challenge. Assessment at day 9 after the last anaesthetic procedure revealed the persistence of the humoral response alteration. Nevertheless, sevo urane-treated animals showed no evidence of histological changes or alteration in hepatic or renal function.

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Section: Discussionmentioning

confidence: 49%

Effects of repetitive sevoflurane anaesthesia on immune response, select biochemical parameters and organ histology in mice

et al. 2003

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“…Food intake and body weight were recorded before the study. Information gathered from previous experimental series showed that the administration of 100% oxygen or halothane 1.5% in oxygen did not alter mice body weight or food intake following treatment [18] .…”

Section: Animalsmentioning

confidence: 95%

Halothane Anesthesia in Mice: Effect on the Phagocytic Activity and Respiratory Burst of Peritoneal Macrophages

Colucci¹,

Harvey²,

Gayol³

et al. 2010

Neuroimmunomodulation

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Objective: To study the effects of halothane anesthesia in mice not undergoing surgery on elements of the inflammatory and stress response; this involved assessment of the phagocytic activity and respiratory burst of peritoneal macrophages as well as plasma corticosterone levels and peripheral leukocyte counts. Methods: There were 2 experimental groups, i.e. mice anesthetized with halothane 1.5% in oxygen for 40 min and a control group of mice subjected to the same manipulations but no anesthesia. At the end of the anesthetic or sham procedure, peritoneal macrophages were evaluated for phagocytic and lytic activity after an immune challenge and spontaneous respiratory burst (chemoluminiscence). Plasma corticosterone and leukocyte counts in peripheral blood were evaluated as indicators of the stress response. Results: In halothane-anesthetized mice, increased numbers and activity of phagocytic cells were found, with regard to the number of ingested and digested particles, compared to the nonanesthetized group. The ex vivo peritoneal macrophage respiratory burst without antigenic stimulation also showed a higher response in anesthetized mice compared with the nonanesthetized controls. Halothane administration did not alter corticosterone levels. Treated and control mice displayed similar leukocyte profiles in peripheral blood, except for lower lymphocyte counts in the controls compared to the halothane group. Typical correlation between corticosterone and leukocyte subsets, together with a high positive correlation between plasma corticosterone and phagocytic cell counts, were found only in the control group. Conclusion: Halothane anesthesia might have beneficial effects on the inflammatory response mediated by phagocytes, namely the activity and efficiency of peritoneal macrophages, in a setting where plasma corticosterone and peripheral leukocyte counts were not affected.

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“…Although no clear anaesthesia-induced modulation of the sensitization of lab rodents to inhaled antigen is reported in the literature, the capacity of inhaled anaesthetics to exert immune-altering function has been demonstrated [51][52][53][54][55][56][57][58][59][60][61][62]. The anaesthetics halothane, isoflurane and methoxyflurane are routinely used for i.n rodent challenges [51,52].…”

Section: Anaesthesiamentioning

confidence: 99%

“…, also reported an enhancement of the anti‐SRBC response in halothane exposed CBi mice, which could originate from a halothane‐driven Th 2 shift. Other, occasionally conflicting, halothane‐induced and immune‐related effects have been reported in rodents, including increased or decreased numbers and activity of phagocytic cells, suppressed T cell function , alteration of cytokine balance as well as reduction of major splenic lymphocyte subsets and splenic IgM and IgG‐secreting spleen cells .…”

Section: Protocol Designmentioning

confidence: 99%

Exposure of immunologically naive laboratory rodents to antigen via the airways. Where does tolerance stop and sensitization begin?

Guibas

Makris

Spandou

et al. 2012

Clin Experimental Allergy

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Conventional rodent models of respiratory allergy that employ intraperitoneal sensitization to aeroallergen plus adjuvant, have offered greatly to our current knowledge of the pathophysiology of allergic airway diseases. Notwithstanding this significant contribution, non-adjuvant aided sensitization via respiratory presentation of the allergen, is more naturally relevant and more closely mimics the human exposure. Nevertheless, in the experimental setting, primary respiratory exposure to inert antigen is likely to lead to inhalation tolerance. Inasmuch as divergent and discrepant results are often reported in experimental models employing this method of sensitization, we set out to review the relative literature and identify and discuss factors that are liable to interfere in such protocols and modify the immune response, hence leading to variable outcomes. Protocol design features (including the use of anaesthesia, the nature and dosage of the antigen and the strain/age/sex and handling of the animals) as well as environmental factors (including airborne substances, viruses and lipopolysaccharide) have been identified as key modulators of the immune response that evolves, following primary airway exposure of laboratory rodents to aeroallergen. Delineation of the effect of those factors to induction or abrogation of inhalation tolerance can have important implications in the design of both improved experimental protocols of respiratory allergy and methods to intercept sensitization to inert aeroallergens in the clinical field.

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Effects of halothane reexposure in female mice and their offspring

Cited by 9 publications

References 37 publications

Effects of repetitive sevoflurane anaesthesia on immune response, select biochemical parameters and organ histology in mice

Effects of repetitive sevoflurane anaesthesia on immune response, select biochemical parameters and organ histology in mice

Halothane Anesthesia in Mice: Effect on the Phagocytic Activity and Respiratory Burst of Peritoneal Macrophages

Exposure of immunologically naive laboratory rodents to antigen via the airways. Where does tolerance stop and sensitization begin?

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