Effects of Hct on L-NAME-induced Potentiation of Anaphylactic Presinusoidal Constriction in Perfused Rat Livers

Cui, Sen; Shibamoto, Toshishige; Liu, Wei; Takano, Haruo; Zhao, Zhansheng; Kurata, Yasutaka

doi:10.1097/01.fjc.0000232063.87708.29

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…We previously showed that almost selective presinusoidal contraction is the characteristic for the hepatic anaphylaxis of isolated SD rat livers perfused with blood (27) or blood-free perfusate (8). We confirmed this previous finding in the present experiment on isolated SD rat livers perfused with ovalbuminKrebs solution.…”

Section: Discussionsupporting

confidence: 82%

The roles of mast cells and Kupffer cells in rat systemic anaphylaxis

Shibamoto

Shimo²,

Cui³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

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Mast cells and other cells such as macrophages have been shown to mediate systemic anaphylaxis. We determined the roles of mast cells and Kupffer cells in hepatic and systemic anaphylaxis of rats. Roles of mast cells were examined by using the mast cell-deficient white spotting (Ws/Ws) rat; the Ws/Ws and wild type (ϩ/ϩ) rats were sensitized with ovalbumin (1 mg). Roles of Kupffer cells were examined by depleting Kupffer cells using gadolinium chloride or liposome-encapsulated dichloromethylene diphosphonate in the Ws/Ws and Sprague-Dawley rats. An intravenous injection of 0.6 mg ovalbumin caused substantial anaphylactic hypotension in both the Ws/Ws and ϩ/ϩ rats; however, the occurrence was delayed in the Ws/Ws rats. After antigen, portal venous pressure increased by 13.1 cmH 2O in the ϩ/ϩ rats, while it increased only by 5.7 cmH2O in the Ws/Ws rats. In response to antigen, the isolated perfused liver of the Ws/Ws rats also showed weak venoconstriction, the magnitude of which was one tenth as large as that of the ϩ/ϩ rats, indicating that hepatic anaphylaxis was primarily due to mast cells. In contrast, Kupffer cell depletion did not attenuate anaphylactic hepatic venoconstriction in isolated perfused livers. In conclusion, mast cells are involved mainly in anaphylactic hepatic presinusoidal portal venoconstriction but only in the early stage of anaphylactic systemic hypotension in rats. Macrophages, including Kupffer cells, do not participate in rat hepatic anaphylactic venoconstriction. blood pressure; hemodynamics; macrophages; anaphylactic shock; hepatic circulation ANAPHYLAXIS IS AN IMMEDIATE, type-1 hypersensitivity reaction that occurs after exposure of sensitized organisms and tissues to sensitizing antigen. The most common life-threatening feature of acute anaphylaxis is cardiovascular collapse and shock (29). Although anaphylaxis is classically mediated by histamine released in response to antigen cross-linking of IgE bound to high-affinity Ig-E receptors, FcεRI, on mast cells, both human and rodent studies indicate that this classical pathway does not account for all anaphylactic responses (3,10,21,26). Indeed, systemic anaphylaxis can be induced in genetically mast cell-deficient mice (13, 15). Moreover, lethal active anaphylactic shock was as likely to develop in mast cell-deficient W/W V as in normal mice (1,13,15,31). In contrast to mice, the role of mast cells in anaphylactic hypotension is not clear for rats: Nishida et al. (22) reported the lack of anaphylactic hypotension in the genetically mast cell-deficient rats "white spotting"; Ws/Ws (23) sensitized with the nematode Nippostrongylus brasiliensis, whereas Guo et al. (12) found similar hypotensive responses in both mast cell-deficient Ws/Ws and their wild-type (ϩ/ϩ) control rats. Thus, the necessary participation of mast cells in anaphylactic hypotension in rats is now being seriously questioned.On the other hand, two distinctive pathways of systemic anaphylaxis have been demonstrated in mice (11, 30): one mediated by mast cells, IgE, FcεRI...

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Section: Discussionsupporting

confidence: 82%

The roles of mast cells and Kupffer cells in rat systemic anaphylaxis

Shibamoto

Shimo²,

Cui³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

Self Cite

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“…12 In the present study, exercise training attenuated anaphylaxisinduced venoconstriction of the isolated liver, although anaphylactic venoconstriction of isolated perfused livers from exercise-trained rats as well as sedentary control rats was augmented by L-NAME, which is consistent with previous results. 23,24 Exercise-induced attenuation of anaphylactic venoconstriction seems to be independent of NO, because L-NAME did not eliminate this effect of exercise. The mechanism underlying the exercise training-induced attenuation of anaphylactic presinusoidal constriction of perfused livers is not known.…”

Section: Discussionmentioning

confidence: 99%

Exercise training attenuates anaphylactic venoconstriction in rat perfused liver, but does not affect anaphylactic hypotension in conscious rats

Cui

Shibamoto

Zhang

et al. 2010

Clin Exp Pharma Physio

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1. Exercise training attenuates circulatory shock due to haemorrhage, endotoxin or heatstroke. However, it remains unknown whether exercise training attenuates anaphylactic shock. Hepatic venoconstriction is involved in rat anaphylactic hypotension. In the present study, we determined the effects of exercise training on both anaphylaxis-induced segmental venoconstriction in rat perfused livers and systemic anaphylaxis in conscious rats. The role of nitric oxide (NO) in the effect of exercise on the venoconstriction of perfused livers was also examined. 2. Rats were subjected to running training on a motorized treadmill for 4 weeks. Two weeks prior to the anaphylaxis experiment, Sprague-Dawley rats were actively sensitized with the antigen ovalbumin. In isolated livers perfused portally with blood, the portal venous pressure (P(pv)) and sinusoidal pressure were measured to determine the pre- and post-sinusoidal resistances (R(pre) and R(post), respectively). In conscious rats, systemic arterial pressure (SAP) and P(pv) were determined. 3. In the perfused livers of sedentary rats, antigen administration led to a predominant presinusoidal constriction, as evidenced by 4.6- and 1.7-fold increases in R(pre) and R(post), respectively. The anaphylaxis-induced increase in R(pre) was significantly attenuated by 24% by exercise training. Inhibition of NO synthase with N(G)-nitro-L-arginine methyl ester (100 micromol/L) 10 min prior to the injection of antigen enhanced anaphylactic venoconstriction, but did not alter the effect of exercise training on the increase in R(pre). In contrast, exercise training did not attenuate either anaphylactic hypotension or portal hypertension in conscious rats. 4. In conclusion, exercise training attenuates the anaphylaxis-induced presinusoidal constriction in rat isolated perfused livers, independent of NO production. However, this action is not evident in conscious rats and exercise training does not affect anaphylactic hypotension in conscious rats.

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Renal response to anaphylaxis in anesthetized rats and isolated perfused rat kidneys: roles of nitric oxide

et al. 2017

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We determined the renal responses to anaphylaxis and the effects of a nitric oxide synthesis inhibitor, L-NAME, in anesthetized rats and isolated perfused rat kidneys. After the ovalbumin antigen injection, the sensitized rats showed transient and substantial decreases in mean blood pressure and renal blood flow and an increase in renal vascular resistance. Creatinine clearance, a measure of renal function, decreased to 53% baseline at 2 h after antigen. L-NAME pretreatment significantly enhanced the antigen-induced renal vasoconstriction and renal dysfunction. Moreover, plasma creatinine levels significantly increased only in the L-NAME pretreated rats. Separately, in isolated perfused kidneys, we observed the antigen-induced renal vasoconstriction and its augmentation by L-NAME. In conclusion, the renal vascular response to the antigen is vasoconstriction, which is enhanced by L-NAME in both isolated perfused rat kidneys and anesthetized rats; it is accompanied by renal dysfunction, which is also augmented by L-NAME.

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Effects of Hct on L-NAME-induced Potentiation of Anaphylactic Presinusoidal Constriction in Perfused Rat Livers

Cited by 6 publications

References 18 publications

The roles of mast cells and Kupffer cells in rat systemic anaphylaxis

The roles of mast cells and Kupffer cells in rat systemic anaphylaxis

Exercise training attenuates anaphylactic venoconstriction in rat perfused liver, but does not affect anaphylactic hypotension in conscious rats

Renal response to anaphylaxis in anesthetized rats and isolated perfused rat kidneys: roles of nitric oxide

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