Effects of heat-killed Lactococcus lactis subsp. lactis JCM 5805 on mucosal and systemic immune parameters, and antiviral reactions to influenza virus in healthy adults; a randomized controlled double-blind study

Fujii, Toshio; Jounai, Kenta; Horie, Akira; Takahashi, Hitoshi; Suzuki, Hiroaki; Ohshio, Konomi; Fujiwara, Daisuke; Yamamoto, Norio

doi:10.1016/j.jff.2017.06.011

Cited by 26 publications

(25 citation statements)

References 46 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Evaluation of influenza and URTI incidence was performed from the severity of symptoms according to a previously reported diagnostic criterion list [ 25 ]. Measurements of severity of each symptoms were performed according to a previous report [ 28 ].…”

Section: Methodsmentioning

confidence: 99%

Efficacy of heat-killed Lactococcus lactis JCM 5805 on immunity and fatigue during consecutive high intensity exercise in male athletes: a randomized, placebo-controlled, double-blinded trial

Komano

Shimada

Naito

et al. 2018

Journal of the International Society of Sports Nutrition

Self Cite

View full text Add to dashboard Cite

BackgroundLactococcus lactis JCM 5805 (LC-Plasma) is a unique lactic acid bacteria (LAB) which activates plasmacytoid dendritic cells (pDC). We aimed to evaluate the effect of LC-Plasma on dendritic cell (DC) activity and subjective indices of upper respiratory tract infections (URTI) and fatigue in athletes under high intensity exercise.MethodsWe conducted a randomized, placebo-controlled, double-blinded trial. Fifty-one male subjects belonging to a university sports club were randomized into placebo (n = 25) and LC-Plasma (n = 26) groups. Individuals ingested placebo capsules containing cornstarch or LC-Plasma capsules containing 100 billion cells of heat-killed LC-Plasma per day for 13 days. During the intervention period, subjects performed high intensity exercise according to their sports club training regime. Blood and saliva sampling were obtained at days 1 and 14, and physical conditions were recorded in a diary. We investigated expression of maturation markers on DCs, muscle damage and stress markers and used student’s t test adjusted by Bonferoni’s method for multiple comparison between groups. These data were presented as mean ± SD. We also investigated cumulative days of symptoms regarding infections and fatigue and used Chi-square test for comparison between groups. These data were presented as cumulative number.ResultsCD86 as maturation marker on pDC was significantly increased in the LC-Plasma group at day 14 (Placebo: 296 ± 70 vs. LC-Plasma: 365 ± 115; Mean Fluorescent Intensity; p = 0.013). Cumulative days of URTI were significantly lower in the LC-Plasma group (Placebo: URTI positive 56, URTI negative 256 vs. LC-Plasma: URTI positive 39, URTI negative 299; days; p = 0.028) and symptoms like sneeze or running nose were significantly lower in the LC-Plasma group (Placebo: Symptom positive 52, Symptom negative 258, vs. LC-Plasma: Symptom positive 36, Symptom negative 301; days; p = 0.032). Moreover, the cumulative days of fatigue were significantly fewer in the LC-Plasma group (Placebo: Symptom positive 128, Symptom negative 182, vs. LC-Plasma: Symptom positive 110, Symptom negative 225; days; p = 0.032). Markers of muscle damage and stress markers were not significantly different between groups.ConclusionWe consider that heat-killed LC-Plasma supplementation relieves morbidity and symptoms of URTI via activation of pDC and decreases fatigue accumulation during consecutive high intensity exercise in athletes. However, LC-Plasma ingestion did not affect markers of muscle damage and stress.Trial registrationUMIN-CTR, UMIN000020372. Registered 28 December 2015.

show abstract

Section: Methodsmentioning

confidence: 99%

Efficacy of heat-killed Lactococcus lactis JCM 5805 on immunity and fatigue during consecutive high intensity exercise in male athletes: a randomized, placebo-controlled, double-blinded trial

Komano

Shimada

Naito

et al. 2018

Journal of the International Society of Sports Nutrition

Self Cite

View full text Add to dashboard Cite

show abstract

“…pDC are immune cells, responsible for early reaction to viral infection and play a key role in preventing the proliferation and spread of viruses by production of type I interferons, a key factor in various immune reactions against viruses [4] [5]. We previously reported that LC-Plasma induced the production of interferon-α (IFN-α) in an in vitro study [3] and prevented viral infection in mouse and human studies [6]- [11]. In addition, oral administration of LC-Plasma significantly suppressed lung inflammation and death caused by parainfluenza virus infection in mice via enhancement of lung anti-viral immunity [6].…”

Section: Introductionmentioning

confidence: 99%

“…In a clinical trial, the expression levels of anti-viral genes in human peripheral blood mononuclear cells (PBMC) stimulated by inactivated human influenza virus A/H1N1 (A/PR/8/34) were higher in the subjects who took LC-Plasma compared with subjects who did not take LC-Plasma [8]. In other clinical studies, the intake of yogurt or supplement containing 50 mg (1.0 × 10 11 cells or more) of LC-Plasma activated human pDC and alleviated the symptoms of flu and common cold [9] [10] [11]. Although it was demonstrated that the intake of 50 mg (1.0 × 10 11 cells or more) of LC-Plasma did not produce any safety concerns, it is assumed that LC-Plasma is taken in excess when it is provided as a supplement.…”

Section: Introductionmentioning

confidence: 99%

Safety Evaluation of Excessive Intake of <i>Lactococcus lactis</i> Subsp. <i>lactis</i> JCM 5805: A Randomized, Double-Blind, Placebo-Controlled, Parallel-Group Trial

Kato¹,

Kanayama²,

S³

et al. 2018

FNS

View full text Add to dashboard Cite

Background/Aims: Administration of a lactic acid bacterial strain, Lactococcus lactis subsp. lactis JCM 5805 (LC-Plasma), is reported to prevent viral infection via activation of plasmacytoid dendritic cells in mouse and human studies. As it is assumed that LC-Plasma is taken in excess when it is commercially provided as a supplement, we conducted a trial using capsules to give 250 mg LC-Plasma (5 times the effective anti-viral dose) every day for four weeks to healthy volunteers to investigate the safety of excessive intake of LC-Plasma. Trial Design: A randomized, double-blind, placebo-controlled, parallel-group trial was conducted. Methods: Forty healthy subjects were randomly assigned to the LC-Plasma group (daily intake of five capsules containing 50 mg heat-killed LC-Plasma cells per capsule) or the placebo group (daily intake of five placebo capsules with no LC-Plasma). Physical, hematological, biochemical and urinary examinations and medical interviews were used to evaluate safety. Results: No abnormal differences were observed after excessive intake of LC-Plasma capsules when compared to the intake of placebo capsules. Conclusions: There are no safety concerns associated with the excessive intake of heat-killed LC-Plasma capsules.

show abstract

“…After 4 continuous weeks administration, L. lactis JCM 5805 significantly reduced cumulative days of symptom of sore throat, compared with control. In L. lactis JCM 5805 group, change in secretory IgA levels in saliva and phagocytic activity of neutrophil were significantly lower than those of initial level, but not in the placebo group, although they could not reach statistical differences between groups at endpoint [ 60 ]. The proposed mechanism of action for L. lactis JCM 5805 is discussed in detail in the next section.…”

Section: Prophylaxis and Medical Treatment Of Infectious Diseases By mentioning

confidence: 99%

Probiotics and Paraprobiotics in Viral Infection: Clinical Application and Effects on the Innate and Acquired Immune Systems

Kanauchi

Andoh

AbuBakar

et al. 2018

CPD

154

124

View full text Add to dashboard Cite

Recently, the risk of viral infection has dramatically increased owing to changes in human ecology such as global warming and an increased geographical movement of people and goods. Howev-er, the efficacy of vaccines and remedies for infectious diseases is limited by the high mutation rates of viruses, especially, RNA viruses. Here, we comprehensively review the effectiveness of several probiot-ics and paraprobiotics (sterilized probiotics) for the prevention or treatment of virally-induced infectious diseases. We discuss the unique roles of these agents in modulating the cross-talk between commensal bacteria and the mucosal immune system. In addition, we provide an overview of the unique mechanism by which viruses are eliminated through the stimulation of type 1 interferon production by probiotics and paraprobiotics via the activation of dendritic cells. Although further detailed research is necessary in the future, probiotics and/or paraprobiotics are expected to be among the rational adjunctive options for the treatment of various viral diseases.

show abstract

Effects of heat-killed Lactococcus lactis subsp. lactis JCM 5805 on mucosal and systemic immune parameters, and antiviral reactions to influenza virus in healthy adults; a randomized controlled double-blind study

Cited by 26 publications

References 46 publications

Efficacy of heat-killed Lactococcus lactis JCM 5805 on immunity and fatigue during consecutive high intensity exercise in male athletes: a randomized, placebo-controlled, double-blinded trial

Efficacy of heat-killed Lactococcus lactis JCM 5805 on immunity and fatigue during consecutive high intensity exercise in male athletes: a randomized, placebo-controlled, double-blinded trial

Safety Evaluation of Excessive Intake of <i>Lactococcus lactis</i> Subsp. <i>lactis</i> JCM 5805: A Randomized, Double-Blind, Placebo-Controlled, Parallel-Group Trial

Probiotics and Paraprobiotics in Viral Infection: Clinical Application and Effects on the Innate and Acquired Immune Systems

Contact Info

Product

Resources

About

Effects of heat-killed Lactococcus lactis subsp. lactis JCM 5805 on mucosal and systemic immune parameters, and antiviral reactions to influenza virus in healthy adults; a randomized controlled double-blind study

Cited by 26 publications

References 46 publications

Efficacy of heat-killed Lactococcus lactis JCM 5805 on immunity and fatigue during consecutive high intensity exercise in male athletes: a randomized, placebo-controlled, double-blinded trial

Efficacy of heat-killed Lactococcus lactis JCM 5805 on immunity and fatigue during consecutive high intensity exercise in male athletes: a randomized, placebo-controlled, double-blinded trial

Safety Evaluation of Excessive Intake of &lt;i&gt;Lactococcus lactis&lt;/i&gt; Subsp. &lt;i&gt;lactis&lt;/i&gt; JCM 5805: A Randomized, Double-Blind, Placebo-Controlled, Parallel-Group Trial

Probiotics and Paraprobiotics in Viral Infection: Clinical Application and Effects on the Innate and Acquired Immune Systems

Contact Info

Product

Resources

About

Safety Evaluation of Excessive Intake of <i>Lactococcus lactis</i> Subsp. <i>lactis</i> JCM 5805: A Randomized, Double-Blind, Placebo-Controlled, Parallel-Group Trial