Effects of heavy ions on the germination and survival of Arabidopsis thaliana

Tanaka, Atsushi; Shikazono, Naoya; Yokota, Yukihiko; Watanabe, Hiroshi; Tano, Shigemitsu

doi:10.1080/095530097143608

Cited by 78 publications

(7 citation statements)

References 7 publications

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“…Ion beams have high linear energy transfer (LET) and higher biological eVects than radiations with low LET such as gamma rays (Fujii et al 1966;Hirono et al 1970;Mei et al 1994;Tanaka et al 1997). Owing to these diVerences, ion beams are expected to have higher mutation induction eVects than gamma rays.…”

Section: Introductionmentioning

confidence: 97%

Mutation induction with ion beam irradiation of lateral buds of chrysanthemum and analysis of chimeric structure of induced mutants

et al. 2008

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We compared the eVects of ion beam and gamma ray irradiation on mutation induction in axillary buds of chrysanthemum, and analyzed the chimeric structure of the resulting mutants. Axillary buds were irradiated with carbon ions at 2 Gy (mean linear energy transfer 122 keV/ m), helium ions at 10 Gy (mean 9 keV/ m), and gamma rays at 80 Gy, all of which have the similar eVects on survival. The lower Wve nodes of the shoots elongating from the irradiated buds were cut one by one, and new shoots were allowed to grow from the axillary buds. This procedure was repeated twice, and Xower color mutation was investigated. Chimeric structure was analyzed by comparing the Xower color of mutants to that of plants regenerated from the roots. Flower color mutants emerged at a high frequency (17.4-28.8%), and there were no signiWcant diVerences in the mutation frequency between the treatments. All the Xower color mutants induced with gamma rays were periclinal chimeras. In contrast, some mutants obtained with ion beams had the same Xower color as that of the plants derived from the roots. This suggests that they were solid mutants, where both LI and LIII tissues were derived from the same mutated cell. Solid mutants were also obtained when irradiated with 5 Gy of helium ions, which had less eVect on survival and mutation than other treatments. Factors for obtaining solid mutants only with ion beams are also discussed.

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Section: Introductionmentioning

confidence: 97%

Mutation induction with ion beam irradiation of lateral buds of chrysanthemum and analysis of chimeric structure of induced mutants

et al. 2008

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“…Several studies have reported that high-LET radiations confer greater and more distinctive biological effects on plant cells than low-LET radiations. For instance, high-LET radiations produce a more lethal effect on Arabidopsis and tobacco cells [ 6 – 9 ]. As for the types of mutation, irradiation of Arabidopsis dry seeds by carbon ion beams induces point-like mutations (base substitutions and several-base deletions/insertions) and chromosomal rearrangements with similar frequencies, whereas electron beams, one of the low-LET forms of radiation, predominantly induce point-like mutations [ 10 , 11 ].…”

Section: Introductionmentioning

confidence: 99%

Mutational effects of γ-rays and carbon ion beams on Arabidopsis seedlings

Yoshihara¹,

Nozawa²,

Hase³

et al. 2013

Journal of Radiation Research

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To assess the mutational effects of radiation on vigorously proliferating plant tissue, the mutation spectrum was analyzed with Arabidopsis seedlings using the plasmid-rescue method. Transgenic plants containing the Escherichia coli rpsL gene were irradiated with γ-rays and carbon ion beams (320-MeV 12C6+), and mutations in the rpsL gene were analyzed. Mutant frequency increased significantly following irradiation by γ-rays, but not by 320-MeV 12C6+. Mutation spectra showed that both radiations increased the frequency of frameshifts and other mutations, including deletions and insertions, but only γ-rays increased the frequency of total base substitutions. These results suggest that the type of DNA lesions which cause base substitutions were less often induced by 320-MeV 12C6+ than by γ-rays in Arabidopsis seedlings. Furthermore, γ-rays never increased the frequencies of G:C to T:A or A:T to C:G transversions, which are caused by oxidized guanine; 320-MeV 12C6+, however, produced a slight increase in both transversions. Instead, γ-rays produced a significant increase in the frequency of G:C to A:T transitions. These results suggest that 8-oxoguanine has little effect on mutagenesis in Arabidopsis cells.

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“…Approximately 150 seeds were placed in a Petri dish with their embryos facing upward. Irradiation apparatus for dry seeds, which was connected to a vertical beam line of an azimuthally varying field (AVF) cyclotron (Japan Atomic Energy Agency, Takasaki, Japan) [27], was used for carbon-ion irradiation with the total energy of 320 MeV (26.7 MeV/u). The linear energy transfer was calculated as 76 keV/μm at the sample surface.…”

Section: Methodsmentioning

confidence: 99%

Isolation of a novel UVB-tolerant rice mutant obtained by exposure to carbon-ion beams

Takano

Takahashi

Yamamoto

et al. 2013

Journal of Radiation Research

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UVB radiation suppresses photosynthesis and protein biosynthesis in plants, which in turn decreases growth and productivity. Here, an ultraviolet-B (UVB)-tolerant rice mutant, utr319 (UV Tolerant Rice 319), was isolated from a mutagenized population derived from 2500 M1 seeds (of the UVB-resistant cultivar ‘Sasanishiki’) that were exposed to carbon ions. The utr319 mutant was more tolerant to UVB than the wild type. Neither the levels of UVB-induced cyclobutane pyrimidine dimers (CPDs) or (6-4) pyrimidine-pyrimidone photodimers [(6-4) photoproducts], nor the repair of CPDs or (6-4) photoproducts, was altered in the utr319 mutant. Thus, the utr319 mutant may be impaired in the production of a previously unidentified factor that confers UVB tolerance. To identify the mutated region in the utr319 mutant, microarray-based comparative genomic hybridization analysis was performed. Two adjacent genes on chromosome 7, Os07g0264900 and Os07g0265100, were predicted to represent the mutant allele. Sequence analysis of the chromosome region in utr319 revealed a deletion of 45 419 bp. RNAi analysis indicated that Os07g0265100 is most likely the mutated gene. Database analysis indicated that the Os07g0265100 gene, UTR319, encodes a putative protein with unknown characteristics or function. In addition, the homologs of UTR319 are conserved only among land plants. Therefore, utr319 is a novel UVB-tolerant rice mutant and UTR319 may be crucial for the determination of UVB sensitivity in rice, although the function of UTR319 has not yet been determined.

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Effects of heavy ions on the germination and survival of Arabidopsis thaliana

Cited by 78 publications

References 7 publications

Mutation induction with ion beam irradiation of lateral buds of chrysanthemum and analysis of chimeric structure of induced mutants

Mutation induction with ion beam irradiation of lateral buds of chrysanthemum and analysis of chimeric structure of induced mutants

Mutational effects of γ-rays and carbon ion beams on Arabidopsis seedlings

Isolation of a novel UVB-tolerant rice mutant obtained by exposure to carbon-ion beams

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