Effects of Helium-Neon Laser Irradiation and Local Anesthetics on Potassium Channels in Pond Snail Neurons

Ignatov, Yu. D.; Вислобоков, А. И.; Власов, Т. Д.; Колпакова, М. Э.; Мельников, К. Н.; Petrishchev, I. N.

doi:10.1007/s11055-005-0137-7

Cited by 18 publications

(9 citation statements)

References 7 publications

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“…In stationary growth phase, the low-intensity red and infrared lasers did not modify the surface areas of wild-type E. coli AB1157, JW1625-1 and JW2146-1 cells ( Table 7 ). Some authors have reported that low-intensity lasers alter the function of ion channels in the plasmatic membrane ( 27 , 28 ) and in the mitochondrial membrane ( 29 ). The results of our morphological analyses can be explained by the effects of the low-intensity lasers on such membrane ion channels.…”

Section: Discussionmentioning

confidence: 99%

Low-intensity red and infrared laser effects at high fluences on Escherichia coli cultures

Barboza¹,

Campos²,

Magalhães³

et al. 2015

Braz J Med Biol Res

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Semiconductor laser devices are readily available and practical radiation sources providing wavelength tenability and high monochromaticity. Low-intensity red and near-infrared lasers are considered safe for use in clinical applications. However, adverse effects can occur via free radical generation, and the biological effects of these lasers from unusually high fluences or high doses have not yet been evaluated. Here, we evaluated the survival, filamentation induction and morphology of Escherichia coli cells deficient in repair of oxidative DNA lesions when exposed to low-intensity red and infrared lasers at unusually high fluences. Cultures of wild-type (AB1157), endonuclease III-deficient (JW1625-1), and endonuclease IV-deficient (JW2146-1) E. coli, in exponential and stationary growth phases, were exposed to red and infrared lasers (0, 250, 500, and 1000 J/cm2) to evaluate their survival rates, filamentation phenotype induction and cell morphologies. The results showed that low-intensity red and infrared lasers at high fluences are lethal, induce a filamentation phenotype, and alter the morphology of the E. coli cells. Low-intensity red and infrared lasers have potential to induce adverse effects on cells, whether used at unusually high fluences, or at high doses. Hence, there is a need to reinforce the importance of accurate dosimetry in therapeutic protocols.

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Section: Discussionmentioning

confidence: 99%

Low-intensity red and infrared laser effects at high fluences on Escherichia coli cultures

Barboza¹,

Campos²,

Magalhães³

et al. 2015

Braz J Med Biol Res

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“…Furthermore, minoxidil is an ATP sensitive K + channel opener which in turn cause hyperpolarization of cell membranes . Since ATP sensitive K + channels in mitochondria and increased levels of NO may have some role to play in effects of LLLT in brain and heart , given what is known about the role of K‐ATP channels and NO in hair regrowth mediated by minoxidil, a mechanistic overlap can be identified. Weiss and co‐workers, by using RT‐PCR and microarray analysis, demonstrated that depending on the treatment parameters, LLLT modulates 5‐α reductase expression, which converts testosterone into DHT, alters vascular endothelial growth factor gene expression as wells as matrix metalloproteinase (MMP‐2) which have significant roles in hair follicle growth, and in turn the group reported stimulation of hair growth on human dermal papillae cells .…”

Section: Hair and Types Of Hair Lossmentioning

confidence: 99%

Low‐level laser (light) therapy (LLLT) for treatment of hair loss

et al. 2013

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Objective Alopecia is a common disorder affecting more than half of the population worldwide. Androgenetic alopecia, the most common type, affects 50% of males over the age of 40 and 75% of females over 65. Only two drugs have been approved so far (minoxidil and finasteride) and hair transplant is the other treatment alternative. This review surveys the evidence for low-level laser therapy (LLLT) applied to the scalp as a treatment for hair loss and discusses possible mechanisms of actions. Methods and Materials Searches of PubMed and Google Scholar were carried out using keywords alopecia, hair loss, LLLT, photobiomodulation. Results Studies have shown that LLLT stimulated hair growth in mice subjected to chemotherapy-induced alopecia and also in alopecia areata. Controlled clinical trials demonstrated that LLLT stimulated hair growth in both men and women. Among various mechanisms, the main mechanism is hypothesized to be stimulation of epidermal stem cells in the hair follicle bulge and shifting the follicles into anagen phase. Conclusion LLLT for hair growth in both men and women appears to be both safe and effective. The optimum wavelength, coherence and dosimetric parameters remain to be determined.

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“…Results from these two mutant E. coli cells confirm that the laser effects depend on the repair of damages to the DNA molecule. 12 On the other hand, a low-level red laser modifies slow potassium currents, 21 and the ion flux through K + and Ca 2+ channels is high following irradiation with a red laser. 22 Moreover, an infrared laser increases transmembrane potential in mitochondria and decreases Ca +2 levels into cells.…”

Section: Discussionmentioning

confidence: 99%

Low-Power Red and Infrared Laser Effects on Cells Deficient in DNA Repair

2019

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Introduction: Low-level lasers are successfully used to prevent and treat diseases in soft oral and bone tissues, particularly diseases in oral cavity caused by chemotherapy and radiotherapy in oncology. However, controversy exists as to whether these lasers induce molecular side effects, mainly on DNA. The aim of this work was to assess the effects of low-power lasers on mutant Escherichia coli cells in DNA repair. Methods: Escherichia coli wild type cultures as well as those lacking recombination DNA repair (recA- ) and la SOS responses (lexA- ) irradiated with lasers at different energy densities, powers, and emission modes for cell viability and morphology assessment were used in this study. Results: Laser irradiation: (i) did not affect cell viability of non-mutant and lexA- cells but decreased viability in recA- cultures; (ii) altered morphology of wild type and lexA, depending on the energy density, power, emission mode, and wavelength. Conclusion: Results show that low-level lasers have lethal effects on both recombination DNA repair and SOS response bacterial cells but do not induce morphological modifications in these cells.

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Effects of Helium-Neon Laser Irradiation and Local Anesthetics on Potassium Channels in Pond Snail Neurons

Cited by 18 publications

References 7 publications

Low-intensity red and infrared laser effects at high fluences on Escherichia coli cultures

Low-intensity red and infrared laser effects at high fluences on Escherichia coli cultures

Low‐level laser (light) therapy (LLLT) for treatment of hair loss

Low-Power Red and Infrared Laser Effects on Cells Deficient in DNA Repair

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