Effects of high glucose on cellular activity of periodontal ligament cells in vitro

Kim, Hyun Sook; Park, Jin Woo; Yeo, Shin Il; Choi, Byung Ju; Suh, Jo Young

doi:10.1016/j.diabres.2006.03.034

Cited by 60 publications

(44 citation statements)

References 33 publications

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“…In addition, glucose may also affect cell proliferation. A lot of studies have demonstrated that high glucose induced cellular senescence, while reduction of glucose enhanced proliferation (Stolzing et al 2006;Kim et al 2006). In our study, the concentration of glucose in RPMI 1640 is two times as high as that in DMEM, but this had not influenced the growth of PDCs.…”

Section: Discussionmentioning

confidence: 68%

Effects of DMEM and RPMI 1640 on the biological behavior of dog periosteum-derived cells

Lin

et al. 2009

Cytotechnology

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Periosteum-derived cells (PDCs) are being extensively studied as potential tissue engineering seed cells and have demonstrated tremendous promise to date. There is convincing evidence that culture medium could modulate the biological behavior of cultured cells. In this study, we investigate the effects of DMEM (low glucose) and RPMI 1640 on cell growth and cell differentiation of PDCs in vitro. PDCs isolated from Beagle dogs were maintained in DMEM and RPMI 1640, respectively. Then, the cell migration rate of periosteum tissues was analyzed. PDCs of the third passage were harvested for the study of proliferation and osteogenic activity. Proliferation was detected by MTT assay. Alkaline phosphatase activity and mineralized nodules were measured to investigate osteogenic differentiation. Our data demonstrated that DMEM induced alkaline phosphatase activity and strongly stimulated matrix mineralization in cell culture, while similar cell migration rates and proliferation behaviors were observed in the two culture conditions. Interestingly, the osteogenic differentiation of PDCs could be enhanced in DMEM compared with that in RPMI 1640. Thus, it can be ascertained that DMEM may serve as a suitable culture condition allowing osteogenic differentiation of dog PDCs.

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Section: Discussionmentioning

confidence: 68%

Effects of DMEM and RPMI 1640 on the biological behavior of dog periosteum-derived cells

Lin

et al. 2009

Cytotechnology

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“…Indeed, glucose may also affect proliferation and differentiation potential of stem cells [52]. Studies by Larsen et al [53], Li et al [54] and Kim et al [55] have demonstrated that high glucose induced cellular senescence, while reduction of glucose enhanced proliferation.…”

Section: Discussionmentioning

confidence: 99%

In Vitro Sustained Differentiation of Rat Colon Epithelial Stem Cells

Abu‐Serie¹,

Demellawy²,

El‐Sayed³

et al. 2016

Biochem Anal Biochem

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Colonic epithelium composes of various cell types including alkaline phosphate-expressing absorptive, mucussecreting goblet and neuroendocrine cells that are derived from stem cells through asymmetric division. The continuous renewal of stem cells occurs under the highly coordinated cellular redox state. In the current study, based on a comparison with other culture media, colon epithelial cells were able to be sustained in vitro with normal status for more than two months under the chosen culture condition; α-MEM medium containing 20% fetal bovine serum. The cultured epithelial cells had normal doubling time and normal morphological characteristics as examined by transmission electron microscope. Also, these cultured cells contained functional stem cells and maintained their differentiation potency of colon stem cells, compared with freshly isolated mucosal epithelial cells, as indicated by the maintaining of aldehyde dehydrogenase 1B1 expression (11.31 ± 0.45 to 11.15 ± 0.48), ability to reduce silver nitrate, alkaline phosphate activity (0.513 ± 0.007 mU/μg to 0.438 ± 0.005 mU/μg), mucin secretion (34.71 ± 0.714 μg/ml to 32.93 ± 0.357 μg/ml) in appropriate cellular redox state level (-258.4 ± 1.3 mV to -237.4 ± 3.7 mV). The present study showed sustaining replication potential and functional differentiation of colonic epithelial stem cell population in this culture. The above culture system may be useful as an in vitro model for stemness, toxicological, and carcinogenesis studies.

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“…19 Rapid bone resorption and marked PDL destruction are features of orthodontic movement in animal models of diabetes in a study done by Li et al, 2 with significantly more osteoclasts in the PDL of diabetic induced rats compared with normoglycemic rats after orthodontic treatment. Graber et al 20 stated that TNF-α plays a prominent role in the mechanism controlling the appearance of osteoclasts at compression sites.…”

Section: Discussionmentioning

confidence: 99%

Effects of infliximab local injection on orthodontic tooth movement in diabetic rabbits: A histological study

Mudhir

Al-Maroof

Jasim

2016

ZJMS

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Effects of high glucose on cellular activity of periodontal ligament cells in vitro

Cited by 60 publications

References 33 publications

Effects of DMEM and RPMI 1640 on the biological behavior of dog periosteum-derived cells

Effects of DMEM and RPMI 1640 on the biological behavior of dog periosteum-derived cells

In Vitro Sustained Differentiation of Rat Colon Epithelial Stem Cells

Effects of infliximab local injection on orthodontic tooth movement in diabetic rabbits: A histological study

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