Effects of high hydrostatic pressure on the growth and β‐carotene production of <i>Rhodotorula glutinis</i>

Wang, Suilou; Chen, Dejing; Deng, Bingyao; Xiao-zong, WU

doi:10.1002/yea.1583

Cited by 10 publications

(4 citation statements)

References 22 publications

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“…The present study found that HHP treatment can have beneficial mutagenic effects on A. tumefaciens for CoQ10 production, which agrees with the finding of Wu et al [21] who found that the bacterial cellulose mutant M 438 was obtained after HHP treatment at 250 MPa for 15 min. Wang et al [19, 20] obtained the barotolerant mutant PR68 after five repeated cycles of HHP treatment at 300 MPa for 12 min. They found that the DNA segments of mutant PR68 were different from the original strain.…”

Section: Resultsmentioning

confidence: 99%

“…There were also reports that HHP treatment in laboratory could cause beneficial mutagenesis to E. coli [17, 18], R. glutinis [19, 20], and G. xylinus [21]. Compared with traditional physical and chemical mutagenesis methods, HHP treatment as a mutagen could offer some advantages such as easier handling, savings in time and money, and negligible effects to the environment [19, 20]. However, little is known related to the effectiveness of HHP treatment on improving the production of CoQ10 in microorganisms.…”

Section: Introductionmentioning

confidence: 99%

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Improvement of Coenzyme Q10 Production: Mutagenesis Induced by High Hydrostatic Pressure Treatment and Optimization of Fermentation Conditions

Yuan

Tian

Yue

2012

Journal of Biomedicine and Biotechnology

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Coenzyme Q10 (CoQ10, ubiquinone), a potent antioxidative dietary supplement, was produced by submerged fermentation using Agrobacterium tumefaciens instead of chemical synthesis or solvent extraction. Agrobacterium tumefaciens 1.2554 was subjected to mutagenesis using a series of treatments including high hydrostatic pressure (HHP) treatment, UV irradiation, and diethyl sulfate (DES) treatment to obtain mutant strains showing higher CoQ10 production than wild-type strains. A mutant strain PK38 with four genetic markers was isolated: the specific CoQ10 content of the mutant strain increased by 52.83% compared with the original strain. Effects of carbon and nitrogen sources on CoQ10 production with PK38 were studied. Sucrose at concentration of 30 g/l was tested as the best carbon source, and yeast extract at concentration of 30 g/l supplemented with 10 g/l of ammonium sulfate was identified to be the most favorable for CoQ10 production using PK38. Fed-batch culture strategy was then used for increasing production of CoQ10 in 5-l fermentor. Using the exponential feeding fed-batch culture of sucrose, cell growth and CoQ10 formation were significantly improved. With this strategy, the final cell biomass, CoQ10 production, and specific CoQ10 production increased by 126.11, 173.12, and 22.76%, respectively, compared to those of batch culture.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Improvement of Coenzyme Q10 Production: Mutagenesis Induced by High Hydrostatic Pressure Treatment and Optimization of Fermentation Conditions

Yuan

Tian

Yue

2012

Journal of Biomedicine and Biotechnology

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“…In the present study, β-carotene was contained in the cell extracts of all four yeasts that enhanced laccase production in co-culture, and no other carotenoids were found. The four yeasts were R. mucilaginosa (Moline et al, 2012), Phaffia rhodozyma (Shi et al, 2014; Chi et al, 2015), S. pararoseus (Han et al, 2012, 2016) and R. glutinis (Wang et al, 2008; Roadjanakamolson and Suntornsuk, 2010), and have been previously reported as β-carotene-producing strains. Therefore, microbial strains that accumulate β-carotene could be the primary agent that enhances laccase production by fungi in a co-culture.…”

Section: Discussionmentioning

confidence: 96%

β-Carotene from Yeasts Enhances Laccase Production of Pleurotus eryngii var. ferulae in Co-culture

et al. 2017

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Laccase is widely used in several industrial applications and co-culture is a common method for enhancing laccase production in submerged fermentation. In this study, the co-culture of four yeasts with Pleurotus eryngii var. ferulae was found to enhance laccase production. An analysis of sterilization temperatures and extraction conditions revealed that the stimulatory compound in yeasts was temperature-sensitive, and that it was fat-soluble. An LC-MS analysis revealed that the possible stimulatory compound for laccase production in the four yeast extracts was β-carotene. Moreover, the addition of 4 mg β-carotene to 150 mL of P. eryngii var. ferulae culture broth improved laccase production by 2.2-fold compared with the control (i.e., a monoculture), and was similar to laccase production in co-culture. In addition, the enhanced laccase production was accompanied by an increase of lac gene transcription, which was 6.2-time higher than the control on the fifth day. Therefore, it was concluded that β-carotene from the co-cultured yeasts enhanced laccase production in P. eryngii var. ferulae, and strains that produce β-carotene could be selected to enhance fungal laccase production in a co-culture. Alternatively, β-carotene or crude extracts of β-carotene could be used to induce high laccase production in large scale.

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“…Factors such as low temperature (20°C), high air flow rate, and high hydrostatic pressure influenced positively β-carotene production by Rhodotorula sp. (Wang et al 2008;Frengova and Beshkova 2009).…”

Section: Yeastsmentioning

confidence: 97%

Technological Aspects of β-Carotene Production

Ribeiro

Barreto

Coelho

2011

Food Bioprocess Technol

140

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β-carotene is the main source of pro-vitamin A and is widely used as a food colorant, with a global market estimated to surpass USD 280 million in 2015. The majority of the β-carotene commercialized in the world is obtained by chemical synthesis from β-ionone. Alternatively, the production of β-carotene can be reached on a biotechnological basis, using filamentous fungi, bacteria, microalgae, and yeasts as producers, or even by extraction from vegetable sources, such as oil palm (Elaeis guineensis) and buriti (Mauritia vinifera). The present review is focused on the current technologies for β-carotene production and presents an overview of new tendencies regarding the carotenoids extraction from microbial and vegetal feedstocks, as well as processes for their concentration.

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Effects of high hydrostatic pressure on the growth and β‐carotene production of Rhodotorula glutinis

Cited by 10 publications

References 22 publications

Improvement of Coenzyme Q10 Production: Mutagenesis Induced by High Hydrostatic Pressure Treatment and Optimization of Fermentation Conditions

Improvement of Coenzyme Q10 Production: Mutagenesis Induced by High Hydrostatic Pressure Treatment and Optimization of Fermentation Conditions

β-Carotene from Yeasts Enhances Laccase Production of Pleurotus eryngii var. ferulae in Co-culture

Technological Aspects of β-Carotene Production

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