Effects of histamine on human periodontal ligament fibroblasts under simulated orthodontic pressure

Abstract

As type-I-allergies show an increasing prevalence in the general populace, orthodontic patients may also be affected by histamine release during treatment. Human periodontal ligament fibroblasts (PDLF) are regulators of orthodontic tooth movement. However, the impact of histamine on PDLF in this regard is unknown. Therefore PDLF were incubated without or with an orthodontic compressive force of 2g/cm 2 with and without additional histamine. To assess the role of histamine-1-receptor (H1R) H1R-antagonist cetirizine was used. Expression of histamine receptors and important mediators of orthodontic tooth movement were investigated. PDLF expressed histamine receptors H1R, H2R and H4R, but not H3R. Histamine increased the expression of H1R, H2R and H4R as well as of interleukin-6, cyclooxygenase-2, and prostaglandin-E2 secretion even without pressure application and induced receptor activator of NF-kB ligand (RANKL) protein expression with unchanged osteoprotegerin secretion. These effects were not observed in presence of H1R antagonist cetirizine. By expressing histamine receptors, PDLF seem to be able to respond to fluctuating histamine levels in the periodontal tissue. Increased histamine concentration was associated with enhanced expression of proinflammatory mediators and RANKL, suggesting an inductive effect of histamine on PDLF-mediated osteoclastogenesis and orthodontic tooth movement. Since cetirizine inhibited these effects, they seem to be mainly mediated via histamine receptor H1R.

Impact of Leptin on Periodontal Ligament Fibroblasts during Mechanical Strain

“…In our study, increased PTGS2 expression was accompanied by elevated RANKL expression due to pressure. OPG secretion was downregulated, which could also been shown in experiments with a similar experimental setup in the past [45][46][47][48]. A significantly increased RANKL expression was detected after compression with leptin treatment.…”

Chiral separation and molecular simulation study of six antihistamine agents on a coated cellulose tri‐(3,5‐dimethylphenycarbamate) column (Chiralcel OD‐RH) and its recognition mechanisms

“…Antihistamine agents can competitively inhibit histamine H 1 ‐receptors so that to antagonize the histamine effects, which have been widely used for the treatment of allergic and ulcer symptoms [7,8]. In this study, six antihistamine drugs (such as orphenadrine, meclizine, terfenadine, mequitazine, dioxopromethazine, and carbinoxamine) containing a chiral carbon atom were selected as the target analytes (see Fig.…”

“…[19] investigated the chiral separation of five antihistamine drugs on Chiralpak IA and Chiralpak ID columns to obtain the complete resolution of meclizine, mequitazine, and carbinoxamine enantiomers. Besides, the enantiomeric separation of meclizine, orphenadrine, terfenadine, and carbinoxamine on three polysaccharide‐based CSPs (Lux Cellulose‐1, Lux Cellulose‐2, and Lux Amylose‐2) has been reported [7,20]. Among these commercially available CSPs materials used for chiral separation in HPLC, polysaccharide‐based CSPs are the most commonly used materials contributed to the high enantioselectivity and excellent loading capacity over a broad range of enantiomers [21–23].…”

Effects of Histamine Receptor Antagonist Cetirizine on Orthodontic Tooth Movement

“…An in vitro study showed that histamine induced the release of prostaglandin E 2 via H1 receptor-mediated Ca 2+ signaling in PDL cells [14]. More recently published research demonstrated that human PDL fibroblasts express H1-, H2-, and H4-receptors [15]. In previous studies a wide variety of drugs, which can be diffusely consumed even without the medical prescription at the same time of an orthodontic treatment, have already been investigated regarding a potential influence on OTM [16]; however, to date little is known about the effects of cetirizine on OTM.…”

“…Due to the prevalence of allergies mediated by the H1-receptor, the use of cetirizine generally increased in the population. Due to its systemic anti-inflammatory action, an impact on OTM is to be considered, as OTM is also a (sterile) inflammatory process at the molecular level mediated by periodontal ligament fibroblasts [31], which have been shown before to change their expression pattern under the influence of histamine [15]. This animal study, therefore, aims to further investigate the influence of the H1-receptor antagonist cetirizine on the rate of OTM, cranial growth, root resorption, and periodontal bone loss in an in vivo rat model of orthodontic tooth movement.…”